Nischarin is differentially expressed in rat brain and regulates neuronal migration.

Nischarin is a protein known to inhibit breast cancer cell motility by regulating the signaling of the Rho GTPase family. However, little is known about its location and function in the nervous system. The aim of the present study was to investigate the regional and cellular expression and functions...

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Autores principales:	Yuemin Ding, Ruyi Zhang, Kena Zhang, Xinyou Lv, Yanan Chen, Aiqing Li, Linlin Wang, Xiong Zhang, Qiang Xia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d76e9e562ef451482ba829fd32bb8aa
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spelling	oai:doaj.org-article:5d76e9e562ef451482ba829fd32bb8aa2021-11-18T08:01:08ZNischarin is differentially expressed in rat brain and regulates neuronal migration.1932-620310.1371/journal.pone.0054563https://doaj.org/article/5d76e9e562ef451482ba829fd32bb8aa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23342172/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Nischarin is a protein known to inhibit breast cancer cell motility by regulating the signaling of the Rho GTPase family. However, little is known about its location and function in the nervous system. The aim of the present study was to investigate the regional and cellular expression and functions of Nischarin in the adult rodent brain. As assessed by real-time PCR, Western blot analysis and immunostaining, we found that Nischarin was widely distributed throughout the brain, with a higher expression in the cerebral cortex and hippocampus. Double-labeling showed that Nischarin was expressed in neurons and was mainly located in the perinuclear region and F-actin-rich protrusions. The expression pattern of Nischarin in the brain was thought to be closely associated with its function. This was verified by our findings from cell migration assays that Nischarin regulated neuronal migration. These results provide a preliminary survey of the distribution of Nischarin in different regions and cell types in the rat brain. This might help to elucidate its physiological roles, and to evaluate its potential clinical implications.Yuemin DingRuyi ZhangKena ZhangXinyou LvYanan ChenAiqing LiLinlin WangXiong ZhangQiang XiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54563 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuemin Ding Ruyi Zhang Kena Zhang Xinyou Lv Yanan Chen Aiqing Li Linlin Wang Xiong Zhang Qiang Xia Nischarin is differentially expressed in rat brain and regulates neuronal migration.
description	Nischarin is a protein known to inhibit breast cancer cell motility by regulating the signaling of the Rho GTPase family. However, little is known about its location and function in the nervous system. The aim of the present study was to investigate the regional and cellular expression and functions of Nischarin in the adult rodent brain. As assessed by real-time PCR, Western blot analysis and immunostaining, we found that Nischarin was widely distributed throughout the brain, with a higher expression in the cerebral cortex and hippocampus. Double-labeling showed that Nischarin was expressed in neurons and was mainly located in the perinuclear region and F-actin-rich protrusions. The expression pattern of Nischarin in the brain was thought to be closely associated with its function. This was verified by our findings from cell migration assays that Nischarin regulated neuronal migration. These results provide a preliminary survey of the distribution of Nischarin in different regions and cell types in the rat brain. This might help to elucidate its physiological roles, and to evaluate its potential clinical implications.
format	article
author	Yuemin Ding Ruyi Zhang Kena Zhang Xinyou Lv Yanan Chen Aiqing Li Linlin Wang Xiong Zhang Qiang Xia
author_facet	Yuemin Ding Ruyi Zhang Kena Zhang Xinyou Lv Yanan Chen Aiqing Li Linlin Wang Xiong Zhang Qiang Xia
author_sort	Yuemin Ding
title	Nischarin is differentially expressed in rat brain and regulates neuronal migration.
title_short	Nischarin is differentially expressed in rat brain and regulates neuronal migration.
title_full	Nischarin is differentially expressed in rat brain and regulates neuronal migration.
title_fullStr	Nischarin is differentially expressed in rat brain and regulates neuronal migration.
title_full_unstemmed	Nischarin is differentially expressed in rat brain and regulates neuronal migration.
title_sort	nischarin is differentially expressed in rat brain and regulates neuronal migration.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5d76e9e562ef451482ba829fd32bb8aa
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Nischarin is differentially expressed in rat brain and regulates neuronal migration.

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