Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable Staphylococcus aureus Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model

Non-typeable (NT) Staphylococcus aureus strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT S. aureus on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice i...

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Autores principales:	Jully Gogoi-Tiwari, Dorji Dorji, Harish Kumar Tiwari, Gayatri Shirolkar, Joshua W. Aleri, Trilochan Mukkur
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> non-typeable biofilm tissue damage inflammation bovine mastitis Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/5d784ff5d5f84d69a60ce78260028fef
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spelling	oai:doaj.org-article:5d784ff5d5f84d69a60ce78260028fef2021-11-25T16:14:08ZPhenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model10.3390/ani111130472076-2615https://doaj.org/article/5d784ff5d5f84d69a60ce78260028fef2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3047https://doaj.org/toc/2076-2615Non-typeable (NT) <i>Staphylococcus aureus</i> strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT <i>S. aureus</i> on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice infected with two different NT <i>S. aureus</i> strains with strong and weak biofilm forming potential demonstrated identical clinical symptoms (moderate), minimal inflammatory infiltrates, and tissue damage (level 1 histopathological changes) in the mammary glands. However, the <i>S. aureus</i> load in the mammary glands of mice and the level of pro-inflammatory cytokines (IL-1β, IL-6, IL-12, IL-17 and IFN-γ) in serum were significantly higher (<i>p</i> ≤ 0.05) in those infected with the strong biofilm forming NT <i>S. aureus</i> strain. The level of IL-6 in sera samples of these mice was extremely high (15,479.9 ± 532 Pg/mL). Furthermore, these mice died in 24h of post infection compared to 30 h in the weak biofilm forming NT <i>S. aureus</i> infected group. The study demonstrates no association between the strength of PIA (polysaccharide intercellular adhesion)-dependent biofilm production by clinical NT <i>S. aureus</i> and mammary gland pathology in a mouse mastitis model. However, the role of biofilm in the virulence of <i>S. aureus</i> advancing the time of mortality in mice warrants further investigation.Jully Gogoi-TiwariDorji DorjiHarish Kumar TiwariGayatri ShirolkarJoshua W. AleriTrilochan MukkurMDPI AGarticle<i>Staphylococcus aureus</i>non-typeablebiofilmtissue damageinflammationbovine mastitisVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3047, p 3047 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> non-typeable biofilm tissue damage inflammation bovine mastitis Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	<i>Staphylococcus aureus</i> non-typeable biofilm tissue damage inflammation bovine mastitis Veterinary medicine SF600-1100 Zoology QL1-991 Jully Gogoi-Tiwari Dorji Dorji Harish Kumar Tiwari Gayatri Shirolkar Joshua W. Aleri Trilochan Mukkur Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
description	Non-typeable (NT) <i>Staphylococcus aureus</i> strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT <i>S. aureus</i> on cytokine production and mammary tissue damage by using a mouse mastitis model. Mice infected with two different NT <i>S. aureus</i> strains with strong and weak biofilm forming potential demonstrated identical clinical symptoms (moderate), minimal inflammatory infiltrates, and tissue damage (level 1 histopathological changes) in the mammary glands. However, the <i>S. aureus</i> load in the mammary glands of mice and the level of pro-inflammatory cytokines (IL-1β, IL-6, IL-12, IL-17 and IFN-γ) in serum were significantly higher (<i>p</i> ≤ 0.05) in those infected with the strong biofilm forming NT <i>S. aureus</i> strain. The level of IL-6 in sera samples of these mice was extremely high (15,479.9 ± 532 Pg/mL). Furthermore, these mice died in 24h of post infection compared to 30 h in the weak biofilm forming NT <i>S. aureus</i> infected group. The study demonstrates no association between the strength of PIA (polysaccharide intercellular adhesion)-dependent biofilm production by clinical NT <i>S. aureus</i> and mammary gland pathology in a mouse mastitis model. However, the role of biofilm in the virulence of <i>S. aureus</i> advancing the time of mortality in mice warrants further investigation.
format	article
author	Jully Gogoi-Tiwari Dorji Dorji Harish Kumar Tiwari Gayatri Shirolkar Joshua W. Aleri Trilochan Mukkur
author_facet	Jully Gogoi-Tiwari Dorji Dorji Harish Kumar Tiwari Gayatri Shirolkar Joshua W. Aleri Trilochan Mukkur
author_sort	Jully Gogoi-Tiwari
title	Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
title_short	Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
title_full	Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
title_fullStr	Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
title_full_unstemmed	Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model
title_sort	phenotypic pia-dependent biofilm production by clinical non-typeable <i>staphylococcus aureus</i> is not associated with the intensity of inflammation in mammary gland: a pilot study using mouse mastitis model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5d784ff5d5f84d69a60ce78260028fef
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Phenotypic PIA-Dependent Biofilm Production by Clinical Non-Typeable <i>Staphylococcus aureus</i> Is Not Associated with the Intensity of Inflammation in Mammary Gland: A Pilot Study Using Mouse Mastitis Model

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