An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall

Abstract In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting nutritional compounds. A previously chara...

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Autores principales:	Mónica Costa, Luís Pio, Pedro Bule, Vânia Cardoso, Cristina M. Alfaia, Diogo Coelho, Joana Brás, Carlos M. G. A. Fontes, José A. M. Prates
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d7b72f574594ee4886b30ddb40f464a
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spelling	oai:doaj.org-article:5d7b72f574594ee4886b30ddb40f464a2021-12-02T16:49:13ZAn individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall10.1038/s41598-021-89278-12045-2322https://doaj.org/article/5d7b72f574594ee4886b30ddb40f464a2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89278-1https://doaj.org/toc/2045-2322Abstract In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting nutritional compounds. A previously characterized individual alginate lyase, belonging to the family 7 of polysaccharide lyases (PL7) and produced by Saccharophagus degradans, was shown to be the most efficient in the in vitro degradation of L. digitata cell wall. The alginate lyase treatment, compared to the control, released up to 7.11 g/L of reducing sugars (p < 0.001) and 8.59 mmol/100 g dried alga of monosaccharides (p < 0.001), and reduced cell wall fluorescence intensity by 39.1% after staining with Calcofluor White (p = 0.001). The hydrolysis of gel-forming polymer alginate by the alginate lyase treatment could prevent the trapping of fatty acids and release beneficial monounsaturated fatty acids, particularly 18:1c9 (p < 0.001), to the extracellular medium. However, no liberation of proteins (p > 0.170) or pigments (p > 0.070) was observed. Overall, these results show the ability of an individual alginate lyase, from PL7 family, to partially degrade L. digitata cell wall under physiological conditions. Therefore, this CAZyme can potentially improve the bioavailability of L. digitata bioactive compounds for monogastric diets, with further application in feed industry.Mónica CostaLuís PioPedro BuleVânia CardosoCristina M. AlfaiaDiogo CoelhoJoana BrásCarlos M. G. A. FontesJosé A. M. PratesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mónica Costa Luís Pio Pedro Bule Vânia Cardoso Cristina M. Alfaia Diogo Coelho Joana Brás Carlos M. G. A. Fontes José A. M. Prates An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
description	Abstract In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting nutritional compounds. A previously characterized individual alginate lyase, belonging to the family 7 of polysaccharide lyases (PL7) and produced by Saccharophagus degradans, was shown to be the most efficient in the in vitro degradation of L. digitata cell wall. The alginate lyase treatment, compared to the control, released up to 7.11 g/L of reducing sugars (p < 0.001) and 8.59 mmol/100 g dried alga of monosaccharides (p < 0.001), and reduced cell wall fluorescence intensity by 39.1% after staining with Calcofluor White (p = 0.001). The hydrolysis of gel-forming polymer alginate by the alginate lyase treatment could prevent the trapping of fatty acids and release beneficial monounsaturated fatty acids, particularly 18:1c9 (p < 0.001), to the extracellular medium. However, no liberation of proteins (p > 0.170) or pigments (p > 0.070) was observed. Overall, these results show the ability of an individual alginate lyase, from PL7 family, to partially degrade L. digitata cell wall under physiological conditions. Therefore, this CAZyme can potentially improve the bioavailability of L. digitata bioactive compounds for monogastric diets, with further application in feed industry.
format	article
author	Mónica Costa Luís Pio Pedro Bule Vânia Cardoso Cristina M. Alfaia Diogo Coelho Joana Brás Carlos M. G. A. Fontes José A. M. Prates
author_facet	Mónica Costa Luís Pio Pedro Bule Vânia Cardoso Cristina M. Alfaia Diogo Coelho Joana Brás Carlos M. G. A. Fontes José A. M. Prates
author_sort	Mónica Costa
title	An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
title_short	An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
title_full	An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
title_fullStr	An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
title_full_unstemmed	An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
title_sort	individual alginate lyase is effective in the disruption of laminaria digitata recalcitrant cell wall
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5d7b72f574594ee4886b30ddb40f464a
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An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall

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