Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus

The fungus, Mucor lusitanicus, is of great interest for microbial lipids, because of its ability to accumulate intracellular lipid using various carbon sources. The biosynthesis of fatty acid requires the reducing power NADPH, and acetyl-CoA, which is produced by the cleavage of citrate in cytosol....

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Autores principales:	Xiuwen Wang, Hassan Mohamed, Yonghong Bao, Chen Wu, Wenyue Shi, Yuanda Song, Junhuan Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	oleaginous fungi malate transporter lipid accumulation Mucor lusitanicus heterologous expression Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5d9414e6940e41b9ad1dcfa8a21df8e9
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spelling	oai:doaj.org-article:5d9414e6940e41b9ad1dcfa8a21df8e92021-11-19T06:47:53ZHeterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus1664-302X10.3389/fmicb.2021.774825https://doaj.org/article/5d9414e6940e41b9ad1dcfa8a21df8e92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.774825/fullhttps://doaj.org/toc/1664-302XThe fungus, Mucor lusitanicus, is of great interest for microbial lipids, because of its ability to accumulate intracellular lipid using various carbon sources. The biosynthesis of fatty acid requires the reducing power NADPH, and acetyl-CoA, which is produced by the cleavage of citrate in cytosol. In this study, we employed different strategies to increase lipid accumulation in the low lipid-producing fungi via metabolic engineering technology. Hence, we constructed the engineered strain of M. lusitanicus CBS 277.49 by using malate transporter (mt) and 2-oxoglutarate: malate antiporter (sodit) from M. circinelloides WJ11. In comparison with the control strain, the lipid content of the overexpressed strains of mt and sodit genes were increased by 24.6 and 33.8%, respectively. These results showed that mt and sodit can affect the distribution of malate in mitochondria and cytosol, provide the substrates for the synthesis of citrate in the mitochondria, and accelerate the transfer of citrate from mitochondria to cytosol, which could play a significant regulatory role in fatty acid synthesis leading to lipids over accumulation.Xiuwen WangHassan MohamedHassan MohamedYonghong BaoChen WuWenyue ShiYuanda SongJunhuan YangFrontiers Media S.A.articleoleaginous fungimalate transporterlipid accumulationMucor lusitanicusheterologous expressionMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oleaginous fungi malate transporter lipid accumulation Mucor lusitanicus heterologous expression Microbiology QR1-502
spellingShingle	oleaginous fungi malate transporter lipid accumulation Mucor lusitanicus heterologous expression Microbiology QR1-502 Xiuwen Wang Hassan Mohamed Hassan Mohamed Yonghong Bao Chen Wu Wenyue Shi Yuanda Song Junhuan Yang Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus
description	The fungus, Mucor lusitanicus, is of great interest for microbial lipids, because of its ability to accumulate intracellular lipid using various carbon sources. The biosynthesis of fatty acid requires the reducing power NADPH, and acetyl-CoA, which is produced by the cleavage of citrate in cytosol. In this study, we employed different strategies to increase lipid accumulation in the low lipid-producing fungi via metabolic engineering technology. Hence, we constructed the engineered strain of M. lusitanicus CBS 277.49 by using malate transporter (mt) and 2-oxoglutarate: malate antiporter (sodit) from M. circinelloides WJ11. In comparison with the control strain, the lipid content of the overexpressed strains of mt and sodit genes were increased by 24.6 and 33.8%, respectively. These results showed that mt and sodit can affect the distribution of malate in mitochondria and cytosol, provide the substrates for the synthesis of citrate in the mitochondria, and accelerate the transfer of citrate from mitochondria to cytosol, which could play a significant regulatory role in fatty acid synthesis leading to lipids over accumulation.
format	article
author	Xiuwen Wang Hassan Mohamed Hassan Mohamed Yonghong Bao Chen Wu Wenyue Shi Yuanda Song Junhuan Yang
author_facet	Xiuwen Wang Hassan Mohamed Hassan Mohamed Yonghong Bao Chen Wu Wenyue Shi Yuanda Song Junhuan Yang
author_sort	Xiuwen Wang
title	Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus
title_short	Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus
title_full	Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus
title_fullStr	Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus
title_full_unstemmed	Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus
title_sort	heterologous expression of two malate transporters from an oleaginous fungus mucor circinelloides improved the lipid accumulation in mucor lusitanicus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5d9414e6940e41b9ad1dcfa8a21df8e9
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Heterologous Expression of Two Malate Transporters From an Oleaginous Fungus Mucor circinelloides Improved the Lipid Accumulation in Mucor lusitanicus

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