Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures

Abstract The establishment of the infant gut microbiota is a highly dynamic process dependent on extrinsic and intrinsic factors. We characterized the faecal microbiota of 4 breastfed infants and 4 formula-fed infants at 17 consecutive time points during the first 12 weeks of life. Microbiota compos...

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Autores principales:	Harro M. Timmerman, Nicole B. M. M. Rutten, Jos Boekhorst, Delphine M. Saulnier, Guus A. M. Kortman, Nikhat Contractor, Martin Kullen, Esther Floris, Hermie J. M. Harmsen, Arine M. Vlieger, Michiel Kleerebezem, Ger T. Rijkers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d979071e1ff429e9a4fc29e9d7b70d5
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spelling	oai:doaj.org-article:5d979071e1ff429e9a4fc29e9d7b70d52021-12-02T11:52:42ZIntestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures10.1038/s41598-017-08268-42045-2322https://doaj.org/article/5d979071e1ff429e9a4fc29e9d7b70d52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08268-4https://doaj.org/toc/2045-2322Abstract The establishment of the infant gut microbiota is a highly dynamic process dependent on extrinsic and intrinsic factors. We characterized the faecal microbiota of 4 breastfed infants and 4 formula-fed infants at 17 consecutive time points during the first 12 weeks of life. Microbiota composition was analysed by a combination of 16S rRNA gene sequencing and quantitative PCR (qPCR). In this dataset, individuality was a major driver of microbiota composition (P = 0.002) and was more pronounced in breastfed infants. A developmental signature could be distinguished, characterized by sequential colonisation of i) intrauterine/vaginal birth associated taxa, ii) skin derived taxa and other typical early colonisers such as Streptococcus and Enterobacteriaceae, iii) domination of Bifidobacteriaceae, and iv) the appearance of adultlike taxa, particularly species associated with Blautia, Eggerthella, and the potential pathobiont Clostridium difficile. Low abundance of potential pathogens was detected by 16S profiling and confirmed by qPCR. Incidence and dominance of skin and breast milk associated microbes were increased in the gut microbiome of breastfed infants compared to formula-fed infants. The approaches in this study indicate that microbiota development of breastfed and formula-fed infants proceeds according to similar developmental stages with microbiota signatures that include stage-specific species.Harro M. TimmermanNicole B. M. M. RuttenJos BoekhorstDelphine M. SaulnierGuus A. M. KortmanNikhat ContractorMartin KullenEsther FlorisHermie J. M. HarmsenArine M. VliegerMichiel KleerebezemGer T. RijkersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Harro M. Timmerman Nicole B. M. M. Rutten Jos Boekhorst Delphine M. Saulnier Guus A. M. Kortman Nikhat Contractor Martin Kullen Esther Floris Hermie J. M. Harmsen Arine M. Vlieger Michiel Kleerebezem Ger T. Rijkers Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
description	Abstract The establishment of the infant gut microbiota is a highly dynamic process dependent on extrinsic and intrinsic factors. We characterized the faecal microbiota of 4 breastfed infants and 4 formula-fed infants at 17 consecutive time points during the first 12 weeks of life. Microbiota composition was analysed by a combination of 16S rRNA gene sequencing and quantitative PCR (qPCR). In this dataset, individuality was a major driver of microbiota composition (P = 0.002) and was more pronounced in breastfed infants. A developmental signature could be distinguished, characterized by sequential colonisation of i) intrauterine/vaginal birth associated taxa, ii) skin derived taxa and other typical early colonisers such as Streptococcus and Enterobacteriaceae, iii) domination of Bifidobacteriaceae, and iv) the appearance of adultlike taxa, particularly species associated with Blautia, Eggerthella, and the potential pathobiont Clostridium difficile. Low abundance of potential pathogens was detected by 16S profiling and confirmed by qPCR. Incidence and dominance of skin and breast milk associated microbes were increased in the gut microbiome of breastfed infants compared to formula-fed infants. The approaches in this study indicate that microbiota development of breastfed and formula-fed infants proceeds according to similar developmental stages with microbiota signatures that include stage-specific species.
format	article
author	Harro M. Timmerman Nicole B. M. M. Rutten Jos Boekhorst Delphine M. Saulnier Guus A. M. Kortman Nikhat Contractor Martin Kullen Esther Floris Hermie J. M. Harmsen Arine M. Vlieger Michiel Kleerebezem Ger T. Rijkers
author_facet	Harro M. Timmerman Nicole B. M. M. Rutten Jos Boekhorst Delphine M. Saulnier Guus A. M. Kortman Nikhat Contractor Martin Kullen Esther Floris Hermie J. M. Harmsen Arine M. Vlieger Michiel Kleerebezem Ger T. Rijkers
author_sort	Harro M. Timmerman
title	Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
title_short	Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
title_full	Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
title_fullStr	Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
title_full_unstemmed	Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
title_sort	intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5d979071e1ff429e9a4fc29e9d7b70d5
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Intestinal colonisation patterns in breastfed and formula-fed infants during the first 12 weeks of life reveal sequential microbiota signatures

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