A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters

Enqi Zhang, Ailing Fu School of Pharmaceutical Sciences, Southwest University, Chongqing, People’s Republic of China Abstract: Despite the significant progress in molecular imaging technologies that has been made in recent years, the specific detection of neural cells still remains chal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang E, Fu A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5d9910247482400d9d383129dd71477b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5d9910247482400d9d383129dd71477b
record_format	dspace
spelling	oai:doaj.org-article:5d9910247482400d9d383129dd71477b2021-12-02T05:09:55ZA new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters1178-2013https://doaj.org/article/5d9910247482400d9d383129dd71477b2015-03-01T00:00:00Zhttp://www.dovepress.com/a-new-strategy-for-specific-imaging-of-neural-cells-based-on-peptide-c-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Enqi Zhang, Ailing Fu School of Pharmaceutical Sciences, Southwest University, Chongqing, People’s Republic of China Abstract: Despite the significant progress in molecular imaging technologies that has been made in recent years, the specific detection of neural cells still remains challenging. Here, we suggest the use of gold nanoclusters (AuNCs) modified with a brain-targeting peptide as a potential imaging candidate for detecting neural cells in vitro and in mice. AuNCs of less than 10 nm (dynamic light scattering analysis) were first prepared using the “green” synthetic approach, and then a targeting peptide, rabies virus glycoprotein derived peptide (RDP), was conjugated to the AuNCs for improving the efficiency and specificity of neural cell penetration. The conjugate’s mechanism of cellular attachment and entry into neural cells was suggested to be receptor-mediated endocytosis through clathrin-coated pits. Also, noninvasive imaging analysis and animal studies indicated that the RDP-modified nanoclusters could concentrate in the brain and locate in neural cells. This study suggests the feasibility of using targeting peptide-modified nanoclusters for noninvasive imaging brain cells in vivo. Keywords: RDP, targeted delivery, bioimaging, brain Zhang EFu ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2115-2124 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang E Fu A A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
description	Enqi Zhang, Ailing Fu School of Pharmaceutical Sciences, Southwest University, Chongqing, People’s Republic of China Abstract: Despite the significant progress in molecular imaging technologies that has been made in recent years, the specific detection of neural cells still remains challenging. Here, we suggest the use of gold nanoclusters (AuNCs) modified with a brain-targeting peptide as a potential imaging candidate for detecting neural cells in vitro and in mice. AuNCs of less than 10 nm (dynamic light scattering analysis) were first prepared using the “green” synthetic approach, and then a targeting peptide, rabies virus glycoprotein derived peptide (RDP), was conjugated to the AuNCs for improving the efficiency and specificity of neural cell penetration. The conjugate’s mechanism of cellular attachment and entry into neural cells was suggested to be receptor-mediated endocytosis through clathrin-coated pits. Also, noninvasive imaging analysis and animal studies indicated that the RDP-modified nanoclusters could concentrate in the brain and locate in neural cells. This study suggests the feasibility of using targeting peptide-modified nanoclusters for noninvasive imaging brain cells in vivo. Keywords: RDP, targeted delivery, bioimaging, brain
format	article
author	Zhang E Fu A
author_facet	Zhang E Fu A
author_sort	Zhang E
title	A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
title_short	A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
title_full	A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
title_fullStr	A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
title_full_unstemmed	A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
title_sort	new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/5d9910247482400d9d383129dd71477b
work_keys_str_mv	AT zhange anewstrategyforspecificimagingofneuralcellsbasedonpeptideconjugatedgoldnanoclusters AT fua anewstrategyforspecificimagingofneuralcellsbasedonpeptideconjugatedgoldnanoclusters AT zhange newstrategyforspecificimagingofneuralcellsbasedonpeptideconjugatedgoldnanoclusters AT fua newstrategyforspecificimagingofneuralcellsbasedonpeptideconjugatedgoldnanoclusters
_version_	1718400531783745536

A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters

Ejemplares similares