Exploring the Bioethanol Production Potential of Miscanthus Cultivars

Miscanthus is a fast-growing perennial grass that attracts significant attention for its potential application as a feedstock for bioethanol production. This report explores the difference in the lignocellulosic composition of various Miscanthus cultivars, inclu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	William Turner, Darren Greetham, Michal Mos, Michael Squance, Jason Kam, Chenyu Du
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Miscanthus</i> × <i>giganteus</i> bioethanol pre-treatment and saccharification yeast fermentation SEM Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/5d9c7b6faa624515929f6d5a2694db3f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5d9c7b6faa624515929f6d5a2694db3f
record_format	dspace
spelling	oai:doaj.org-article:5d9c7b6faa624515929f6d5a2694db3f2021-11-11T15:02:28ZExploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars10.3390/app112199492076-3417https://doaj.org/article/5d9c7b6faa624515929f6d5a2694db3f2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9949https://doaj.org/toc/2076-3417<i>Miscanthus</i> is a fast-growing perennial grass that attracts significant attention for its potential application as a feedstock for bioethanol production. This report explores the difference in the lignocellulosic composition of various <i>Miscanthus</i> cultivars, including <i>Miscanthus × giganteus</i> cultivated at the same location (mainly Lincoln, UK). It also assesses the sugar release profiles and mineral composition profiles of five <i>Miscanthus</i> cultivars harvested over a growing period from November 2018 to February 2019. The results showed that <i>Miscanthus × giganteus</i> contains approximately 45.5% cellulose, 29.2% hemicellulose and 23.8% lignin (dry weight, <i>w</i>/<i>w</i>). Other cultivars of <i>Miscanthus</i> also contain high quantities of carbohydrates (cellulose 41.1–46.0%, hemicellulose 24.3–32.6% and lignin 21.4–24.9%). Pre-treatment of <i>Miscanthus</i> using dilute acid followed by enzymatic hydrolysis released 63.7–80.2% of the theoretical glucose content. Fermentation of a hydrolysate of <i>Miscanthus</i> × <i>giganteus</i> using <i>Saccharomyces cerevisiae</i> NCYC2592 produced 13.58 ± 1.11 g/L of ethanol from 35.13 ± 0.46 g/L of glucose, corresponding to a yield of 0.148 g/g dry weight <i>Miscanthus</i> biomass. Scanning electron microscopy was used to study the morphology of raw and hydrolysed <i>Miscanthus</i> samples, which provided visual proof of <i>Miscanthus</i> lignocellulose degradation in these processes. The sugar release profile showed that a consequence of <i>Miscanthus</i> plant growth is an increase in difficulty in releasing monosaccharides from the biomass. The potassium, magnesium, sodium, sulphur and phosphorus contents in various <i>Miscanthus</i> cultivars were analysed. The results revealed that these elements were slowly lost from the plants during the latter part of the growing season, for a specific cultivar, until February 2019.William TurnerDarren GreethamMichal MosMichael SquanceJason KamChenyu DuMDPI AGarticle<i>Miscanthus</i> × <i>giganteus</i>bioethanolpre-treatment and saccharificationyeast fermentationSEMTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9949, p 9949 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Miscanthus</i> × <i>giganteus</i> bioethanol pre-treatment and saccharification yeast fermentation SEM Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	<i>Miscanthus</i> × <i>giganteus</i> bioethanol pre-treatment and saccharification yeast fermentation SEM Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 William Turner Darren Greetham Michal Mos Michael Squance Jason Kam Chenyu Du Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars
description	<i>Miscanthus</i> is a fast-growing perennial grass that attracts significant attention for its potential application as a feedstock for bioethanol production. This report explores the difference in the lignocellulosic composition of various <i>Miscanthus</i> cultivars, including <i>Miscanthus × giganteus</i> cultivated at the same location (mainly Lincoln, UK). It also assesses the sugar release profiles and mineral composition profiles of five <i>Miscanthus</i> cultivars harvested over a growing period from November 2018 to February 2019. The results showed that <i>Miscanthus × giganteus</i> contains approximately 45.5% cellulose, 29.2% hemicellulose and 23.8% lignin (dry weight, <i>w</i>/<i>w</i>). Other cultivars of <i>Miscanthus</i> also contain high quantities of carbohydrates (cellulose 41.1–46.0%, hemicellulose 24.3–32.6% and lignin 21.4–24.9%). Pre-treatment of <i>Miscanthus</i> using dilute acid followed by enzymatic hydrolysis released 63.7–80.2% of the theoretical glucose content. Fermentation of a hydrolysate of <i>Miscanthus</i> × <i>giganteus</i> using <i>Saccharomyces cerevisiae</i> NCYC2592 produced 13.58 ± 1.11 g/L of ethanol from 35.13 ± 0.46 g/L of glucose, corresponding to a yield of 0.148 g/g dry weight <i>Miscanthus</i> biomass. Scanning electron microscopy was used to study the morphology of raw and hydrolysed <i>Miscanthus</i> samples, which provided visual proof of <i>Miscanthus</i> lignocellulose degradation in these processes. The sugar release profile showed that a consequence of <i>Miscanthus</i> plant growth is an increase in difficulty in releasing monosaccharides from the biomass. The potassium, magnesium, sodium, sulphur and phosphorus contents in various <i>Miscanthus</i> cultivars were analysed. The results revealed that these elements were slowly lost from the plants during the latter part of the growing season, for a specific cultivar, until February 2019.
format	article
author	William Turner Darren Greetham Michal Mos Michael Squance Jason Kam Chenyu Du
author_facet	William Turner Darren Greetham Michal Mos Michael Squance Jason Kam Chenyu Du
author_sort	William Turner
title	Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars
title_short	Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars
title_full	Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars
title_fullStr	Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars
title_full_unstemmed	Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars
title_sort	exploring the bioethanol production potential of <i>miscanthus</i> cultivars
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5d9c7b6faa624515929f6d5a2694db3f
work_keys_str_mv	AT williamturner exploringthebioethanolproductionpotentialofimiscanthusicultivars AT darrengreetham exploringthebioethanolproductionpotentialofimiscanthusicultivars AT michalmos exploringthebioethanolproductionpotentialofimiscanthusicultivars AT michaelsquance exploringthebioethanolproductionpotentialofimiscanthusicultivars AT jasonkam exploringthebioethanolproductionpotentialofimiscanthusicultivars AT chenyudu exploringthebioethanolproductionpotentialofimiscanthusicultivars
_version_	1718437314242281472

Exploring the Bioethanol Production Potential of <i>Miscanthus</i> Cultivars

Ejemplares similares