Application and characterization of crude fungal lipases used to degrade fat and oil wastes

Abstract Aspergillus niger MH078571.1 and A. niger MH079049.1 were identified previously as the two highest Aspergillus niger strains producing lipase. Biochemical characterizations of lipase activity and stability for these two strains were examined and revealed that the optimal temperature is 45 °...

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Autores principales:	Amira Hassan Alabdalall, Norah A. Al-Anazi, Lena A. Aldakheel, Fatma H. I. Amer, Fatimah A. Aldakheel, Ibtisam M. Ababutain, Azzah I. Alghamdi, Eida M. Al-Khaldi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5db019599daf439eb82c3ac2c7ad0cf6
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spelling	oai:doaj.org-article:5db019599daf439eb82c3ac2c7ad0cf62021-12-02T18:01:48ZApplication and characterization of crude fungal lipases used to degrade fat and oil wastes10.1038/s41598-021-98927-42045-2322https://doaj.org/article/5db019599daf439eb82c3ac2c7ad0cf62021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98927-4https://doaj.org/toc/2045-2322Abstract Aspergillus niger MH078571.1 and A. niger MH079049.1 were identified previously as the two highest Aspergillus niger strains producing lipase. Biochemical characterizations of lipase activity and stability for these two strains were examined and revealed that the optimal temperature is 45 °C at pH 8for A. niger MH078571.1 and 55 °C for MH079049.1. The lipase production of both strains was studied on medium contains waste oil, as a cheap source to reduce the industrial cost, showed that the optimal incubation period for the enzyme production is 3 days. Moreover, an experiment on lipase activates in organic solvents demonstrated that 50% of acetone is the best solvent for the two strains. In the presence of surfactants, 0.1% of tween 80 surfactant showed the best lipase activities. Furthermore, Mg2+ and Zn2+ ions enhanced the lipase activity of A. niger MH078571.1, while Na2+ and Cu2+ enhanced the enzyme activity of A. niger MH079049.1. Lipase activity was also tested for industrial applications such as integrating it with different detergents. Maximum lipase activity was obtained with 1% of Omo as a powder detergent for both strains. In liquid detergent, 0.1% of Fairy showed maximum lipase activity in A. niger MH078571.1, while the lipase in A. niger MH079049.1 was more effective in 1% of Lux. Moreover, the degradation of natural animal fat with crude enzyme was tested using chicken and sheep fats. The results showed that more than 90% of fats degraded after 5 days of the incubation period.Amira Hassan AlabdalallNorah A. Al-AnaziLena A. AldakheelFatma H. I. AmerFatimah A. AldakheelIbtisam M. AbabutainAzzah I. AlghamdiEida M. Al-KhaldiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amira Hassan Alabdalall Norah A. Al-Anazi Lena A. Aldakheel Fatma H. I. Amer Fatimah A. Aldakheel Ibtisam M. Ababutain Azzah I. Alghamdi Eida M. Al-Khaldi Application and characterization of crude fungal lipases used to degrade fat and oil wastes
description	Abstract Aspergillus niger MH078571.1 and A. niger MH079049.1 were identified previously as the two highest Aspergillus niger strains producing lipase. Biochemical characterizations of lipase activity and stability for these two strains were examined and revealed that the optimal temperature is 45 °C at pH 8for A. niger MH078571.1 and 55 °C for MH079049.1. The lipase production of both strains was studied on medium contains waste oil, as a cheap source to reduce the industrial cost, showed that the optimal incubation period for the enzyme production is 3 days. Moreover, an experiment on lipase activates in organic solvents demonstrated that 50% of acetone is the best solvent for the two strains. In the presence of surfactants, 0.1% of tween 80 surfactant showed the best lipase activities. Furthermore, Mg2+ and Zn2+ ions enhanced the lipase activity of A. niger MH078571.1, while Na2+ and Cu2+ enhanced the enzyme activity of A. niger MH079049.1. Lipase activity was also tested for industrial applications such as integrating it with different detergents. Maximum lipase activity was obtained with 1% of Omo as a powder detergent for both strains. In liquid detergent, 0.1% of Fairy showed maximum lipase activity in A. niger MH078571.1, while the lipase in A. niger MH079049.1 was more effective in 1% of Lux. Moreover, the degradation of natural animal fat with crude enzyme was tested using chicken and sheep fats. The results showed that more than 90% of fats degraded after 5 days of the incubation period.
format	article
author	Amira Hassan Alabdalall Norah A. Al-Anazi Lena A. Aldakheel Fatma H. I. Amer Fatimah A. Aldakheel Ibtisam M. Ababutain Azzah I. Alghamdi Eida M. Al-Khaldi
author_facet	Amira Hassan Alabdalall Norah A. Al-Anazi Lena A. Aldakheel Fatma H. I. Amer Fatimah A. Aldakheel Ibtisam M. Ababutain Azzah I. Alghamdi Eida M. Al-Khaldi
author_sort	Amira Hassan Alabdalall
title	Application and characterization of crude fungal lipases used to degrade fat and oil wastes
title_short	Application and characterization of crude fungal lipases used to degrade fat and oil wastes
title_full	Application and characterization of crude fungal lipases used to degrade fat and oil wastes
title_fullStr	Application and characterization of crude fungal lipases used to degrade fat and oil wastes
title_full_unstemmed	Application and characterization of crude fungal lipases used to degrade fat and oil wastes
title_sort	application and characterization of crude fungal lipases used to degrade fat and oil wastes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5db019599daf439eb82c3ac2c7ad0cf6
work_keys_str_mv	AT amirahassanalabdalall applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT norahaalanazi applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT lenaaaldakheel applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT fatmahiamer applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT fatimahaaldakheel applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT ibtisammababutain applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT azzahialghamdi applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes AT eidamalkhaldi applicationandcharacterizationofcrudefungallipasesusedtodegradefatandoilwastes
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Application and characterization of crude fungal lipases used to degrade fat and oil wastes

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