Determination of N-Acetyl-l-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands

A new flow injection spectrophotometric method for the determination of N-acetyl-l-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chro...

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Autores principales:	Lea Kukoc-Modun, Tomislav Kraljević, Dimitrios Tsikas, Njegomir Radić, Darko Modun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl ester neocuproine bicinchoninic acid bathocuproine disulfonic acid flow injection analysis spectrophotometric determination Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/5db6d4ff657a4f0aa4b6d8062605c105
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spelling	oai:doaj.org-article:5db6d4ff657a4f0aa4b6d8062605c1052021-11-25T18:27:32ZDetermination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands10.3390/molecules262268261420-3049https://doaj.org/article/5db6d4ff657a4f0aa4b6d8062605c1052021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6826https://doaj.org/toc/1420-3049A new flow injection spectrophotometric method for the determination of <i>N</i>-acetyl-<span style="font-variant: small-caps;">l</span>-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chromophoric Cu(I)-ligand complexes with the analyte. The studied ligands were neocuproine (NCN), bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The absorbance of the Cu(I)-ligand complex was measured at 458, 562 and 483 nm for the reactions of NACET with NCN, BCA and BCS, respectively. The method was validated in terms of linear dynamic range, limit of detection and quantitation, accuracy, selectivity, and precision. Experimental conditions were optimized by a univariate method, yielding linear calibration curves in a concentration range from 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 2.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using NCN; 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 1.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCA and 6.0 × 10<sup>−7</sup> mol L<sup>−1</sup> to 1.2 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCS. The achieved analytical frequency was 90 h<sup>−1</sup> for all three ligands. The method was successfully employed for NACET determination in pharmaceutical preparations, indicating that this FIA method fulfilled all the essential demands for the determination of NACET in quality control laboratories, as it combined low instrument and reagent costs with a high sampling rate.Lea Kukoc-ModunTomislav KraljevićDimitrios TsikasNjegomir RadićDarko ModunMDPI AGarticle<i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl esterneocuproinebicinchoninic acidbathocuproine disulfonic acidflow injection analysisspectrophotometric determinationOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6826, p 6826 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl ester neocuproine bicinchoninic acid bathocuproine disulfonic acid flow injection analysis spectrophotometric determination Organic chemistry QD241-441
spellingShingle	<i>N</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl ester neocuproine bicinchoninic acid bathocuproine disulfonic acid flow injection analysis spectrophotometric determination Organic chemistry QD241-441 Lea Kukoc-Modun Tomislav Kraljević Dimitrios Tsikas Njegomir Radić Darko Modun Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
description	A new flow injection spectrophotometric method for the determination of <i>N</i>-acetyl-<span style="font-variant: small-caps;">l</span>-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chromophoric Cu(I)-ligand complexes with the analyte. The studied ligands were neocuproine (NCN), bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The absorbance of the Cu(I)-ligand complex was measured at 458, 562 and 483 nm for the reactions of NACET with NCN, BCA and BCS, respectively. The method was validated in terms of linear dynamic range, limit of detection and quantitation, accuracy, selectivity, and precision. Experimental conditions were optimized by a univariate method, yielding linear calibration curves in a concentration range from 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 2.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using NCN; 2.0 × 10<sup>−6</sup> mol L<sup>−1</sup> to 1.0 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCA and 6.0 × 10<sup>−7</sup> mol L<sup>−1</sup> to 1.2 × 10<sup>−4</sup> mol L<sup>−1</sup> using BCS. The achieved analytical frequency was 90 h<sup>−1</sup> for all three ligands. The method was successfully employed for NACET determination in pharmaceutical preparations, indicating that this FIA method fulfilled all the essential demands for the determination of NACET in quality control laboratories, as it combined low instrument and reagent costs with a high sampling rate.
format	article
author	Lea Kukoc-Modun Tomislav Kraljević Dimitrios Tsikas Njegomir Radić Darko Modun
author_facet	Lea Kukoc-Modun Tomislav Kraljević Dimitrios Tsikas Njegomir Radić Darko Modun
author_sort	Lea Kukoc-Modun
title	Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
title_short	Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
title_full	Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
title_fullStr	Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
title_full_unstemmed	Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
title_sort	determination of <i>n</i>-acetyl-<span style="font-variant: small-caps">l</span>-cysteine ethyl ester (nacet) by flow injection analysis and spectrophotometric detection using different thiol-sensitive ligands
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5db6d4ff657a4f0aa4b6d8062605c105
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Determination of <i>N</i>-Acetyl-<span style="font-variant: small-caps">l</span>-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands

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