Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription

FUS/EWS/TAF15 (FET) fusion oncoproteins contain low complexity domain which forms biomolecular condensates that recruit RNA polymerase II. Here the authors develop a single-molecule assay to visualize this phenomenon providing in vitro evidence to support causative relationship between the formation...

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Autores principales:	Linyu Zuo, Guanwei Zhang, Matthew Massett, Jun Cheng, Zicong Guo, Liang Wang, Yifei Gao, Ru Li, Xu Huang, Pilong Li, Zhi Qi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5dcc0a21b5b445ffb93714c22a52cc70
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spelling	oai:doaj.org-article:5dcc0a21b5b445ffb93714c22a52cc702021-12-02T15:52:56ZLoci-specific phase separation of FET fusion oncoproteins promotes gene transcription10.1038/s41467-021-21690-72041-1723https://doaj.org/article/5dcc0a21b5b445ffb93714c22a52cc702021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21690-7https://doaj.org/toc/2041-1723FUS/EWS/TAF15 (FET) fusion oncoproteins contain low complexity domain which forms biomolecular condensates that recruit RNA polymerase II. Here the authors develop a single-molecule assay to visualize this phenomenon providing in vitro evidence to support causative relationship between the formation of condensates on DNA and gene transcription. Furthermore, they also determine a threshold number of fusion-binding DNA satellite elements required for the formation of FET protein condensates.Linyu ZuoGuanwei ZhangMatthew MassettJun ChengZicong GuoLiang WangYifei GaoRu LiXu HuangPilong LiZhi QiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Linyu Zuo Guanwei Zhang Matthew Massett Jun Cheng Zicong Guo Liang Wang Yifei Gao Ru Li Xu Huang Pilong Li Zhi Qi Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
description	FUS/EWS/TAF15 (FET) fusion oncoproteins contain low complexity domain which forms biomolecular condensates that recruit RNA polymerase II. Here the authors develop a single-molecule assay to visualize this phenomenon providing in vitro evidence to support causative relationship between the formation of condensates on DNA and gene transcription. Furthermore, they also determine a threshold number of fusion-binding DNA satellite elements required for the formation of FET protein condensates.
format	article
author	Linyu Zuo Guanwei Zhang Matthew Massett Jun Cheng Zicong Guo Liang Wang Yifei Gao Ru Li Xu Huang Pilong Li Zhi Qi
author_facet	Linyu Zuo Guanwei Zhang Matthew Massett Jun Cheng Zicong Guo Liang Wang Yifei Gao Ru Li Xu Huang Pilong Li Zhi Qi
author_sort	Linyu Zuo
title	Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
title_short	Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
title_full	Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
title_fullStr	Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
title_full_unstemmed	Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
title_sort	loci-specific phase separation of fet fusion oncoproteins promotes gene transcription
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5dcc0a21b5b445ffb93714c22a52cc70
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Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription

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