Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.

Transmission is a matter of life or death for pathogen lineages and can therefore be considered as the main motor of their evolution. Gammaherpesviruses are archetypal pathogenic persistent viruses which have evolved to be transmitted in presence of specific immune response. Identifying their mode o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sylvie François, Sarah Vidick, Mickaël Sarlet, Daniel Desmecht, Pierre Drion, Philip G Stevenson, Alain Vanderplasschen, Laurent Gillet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5dccf3baf9294b77ac96dc4641f093e3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5dccf3baf9294b77ac96dc4641f093e3
record_format	dspace
spelling	oai:doaj.org-article:5dccf3baf9294b77ac96dc4641f093e32021-11-18T06:05:48ZIllumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.1553-73661553-737410.1371/journal.ppat.1003292https://doaj.org/article/5dccf3baf9294b77ac96dc4641f093e32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593002/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Transmission is a matter of life or death for pathogen lineages and can therefore be considered as the main motor of their evolution. Gammaherpesviruses are archetypal pathogenic persistent viruses which have evolved to be transmitted in presence of specific immune response. Identifying their mode of transmission and their mechanisms of immune evasion is therefore essential to develop prophylactic and therapeutic strategies against these infections. As the known human gammaherpesviruses, Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus are host-specific and lack a convenient in vivo infection model; related animal gammaherpesviruses, such as murine gammaherpesvirus-68 (MHV-68), are commonly used as general models of gammaherpesvirus infections in vivo. To date, it has however never been possible to monitor viral excretion or virus transmission of MHV-68 in laboratory mice population. In this study, we have used MHV-68 associated with global luciferase imaging to investigate potential excretion sites of this virus in laboratory mice. This allowed us to identify a genital excretion site of MHV-68 following intranasal infection and latency establishment in female mice. This excretion occurred at the external border of the vagina and was dependent on the presence of estrogens. However, MHV-68 vaginal excretion was not associated with vertical transmission to the litter or with horizontal transmission to female mice. In contrast, we observed efficient virus transmission to naïve males after sexual contact. In vivo imaging allowed us to show that MHV-68 firstly replicated in penis epithelium and corpus cavernosum before spreading to draining lymph nodes and spleen. All together, those results revealed the first experimental transmission model for MHV-68 in laboratory mice. In the future, this model could help us to better understand the biology of gammaherpesviruses and could also allow the development of strategies that could prevent the spread of these viruses in natural populations.Sylvie FrançoisSarah VidickMickaël SarletDaniel DesmechtPierre DrionPhilip G StevensonAlain VanderplasschenLaurent GilletPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 4, p e1003292 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Sylvie François Sarah Vidick Mickaël Sarlet Daniel Desmecht Pierre Drion Philip G Stevenson Alain Vanderplasschen Laurent Gillet Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
description	Transmission is a matter of life or death for pathogen lineages and can therefore be considered as the main motor of their evolution. Gammaherpesviruses are archetypal pathogenic persistent viruses which have evolved to be transmitted in presence of specific immune response. Identifying their mode of transmission and their mechanisms of immune evasion is therefore essential to develop prophylactic and therapeutic strategies against these infections. As the known human gammaherpesviruses, Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus are host-specific and lack a convenient in vivo infection model; related animal gammaherpesviruses, such as murine gammaherpesvirus-68 (MHV-68), are commonly used as general models of gammaherpesvirus infections in vivo. To date, it has however never been possible to monitor viral excretion or virus transmission of MHV-68 in laboratory mice population. In this study, we have used MHV-68 associated with global luciferase imaging to investigate potential excretion sites of this virus in laboratory mice. This allowed us to identify a genital excretion site of MHV-68 following intranasal infection and latency establishment in female mice. This excretion occurred at the external border of the vagina and was dependent on the presence of estrogens. However, MHV-68 vaginal excretion was not associated with vertical transmission to the litter or with horizontal transmission to female mice. In contrast, we observed efficient virus transmission to naïve males after sexual contact. In vivo imaging allowed us to show that MHV-68 firstly replicated in penis epithelium and corpus cavernosum before spreading to draining lymph nodes and spleen. All together, those results revealed the first experimental transmission model for MHV-68 in laboratory mice. In the future, this model could help us to better understand the biology of gammaherpesviruses and could also allow the development of strategies that could prevent the spread of these viruses in natural populations.
format	article
author	Sylvie François Sarah Vidick Mickaël Sarlet Daniel Desmecht Pierre Drion Philip G Stevenson Alain Vanderplasschen Laurent Gillet
author_facet	Sylvie François Sarah Vidick Mickaël Sarlet Daniel Desmecht Pierre Drion Philip G Stevenson Alain Vanderplasschen Laurent Gillet
author_sort	Sylvie François
title	Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
title_short	Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
title_full	Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
title_fullStr	Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
title_full_unstemmed	Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
title_sort	illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5dccf3baf9294b77ac96dc4641f093e3
work_keys_str_mv	AT sylviefrancois illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT sarahvidick illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT mickaelsarlet illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT danieldesmecht illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT pierredrion illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT philipgstevenson illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT alainvanderplasschen illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice AT laurentgillet illuminationofmurinegammaherpesvirus68cyclerevealsasexualtransmissionroutefromfemalestomalesinlaboratorymice
_version_	1718424604470411264

Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice.

Ejemplares similares