Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation

Background: The previous studies demonstrated that there might be complex and close relationships among leucine supplementation, gut microbiota, and muscle health, which still needs further investigation.Aims: This study aimed to explore the associations of gut microbiota with muscle health after le...

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Autores principales:	Yuxiao Liao, Dan Li, Xiaolei Zhou, Zhao Peng, Zitong Meng, Rui Liu, Wei Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	leucine muscle health gut microbiota aging mice pyruvate Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5dd587e2be8f46139cf16d99812f1ac8
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spelling	oai:doaj.org-article:5dd587e2be8f46139cf16d99812f1ac82021-11-22T05:00:19ZPyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation2296-858X10.3389/fmed.2021.755803https://doaj.org/article/5dd587e2be8f46139cf16d99812f1ac82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.755803/fullhttps://doaj.org/toc/2296-858XBackground: The previous studies demonstrated that there might be complex and close relationships among leucine supplementation, gut microbiota, and muscle health, which still needs further investigation.Aims: This study aimed to explore the associations of gut microbiota with muscle health after leucine intake.Methods: In this study, 19-month-old male C57BL/6j mice (n = 12/group) were supplemented with ultrapure water, low dose of leucine (500 mg/kg·d), and high dose of leucine (1,250 mg/kg·d) for 12 weeks by oral gavage. The mice fecal samples in each group before and after supplementation were collected for baseline and endpoint gut microbiota analysis by using 16S rDNA amplicon sequencing. Meanwhile, ultrasound measurement, H&E staining, myofiber cross-sectional area (CSA) measurement, and western blotting were performed in the quadriceps subsequently. The pyruvate levels were detected in feces.Results: Improvement in muscle of histology and ultrasonography were observed after both low and high dose of leucine supplementation. High dose of leucine supplementation could promote skeletal muscle health in aging mice via regulating AMPKα/SIRT1/PGC-1α. The richness and diversities of microbiota as well as enriched metabolic pathways were altered after leucine supplementation. Firmicutes-Bacteroidetes ratio was significantly decreased in high-leucine group. Moreover, pyruvate fermentation to propanoate I were negatively associated with differential species and the pyruvate levels were significantly increased in feces after high dose of leucine supplementation.Conclusions: Chronic high dose of leucine supplementation changed gut microbiota composition and increased pyruvate levels in the feces, which possibly provides a novel direction for promoting muscle health in aging mice.Yuxiao LiaoYuxiao LiaoDan LiDan LiXiaolei ZhouXiaolei ZhouZhao PengZhao PengZitong MengZitong MengRui LiuWei YangWei YangFrontiers Media S.A.articleleucinemuscle healthgut microbiotaaging micepyruvateMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	leucine muscle health gut microbiota aging mice pyruvate Medicine (General) R5-920
spellingShingle	leucine muscle health gut microbiota aging mice pyruvate Medicine (General) R5-920 Yuxiao Liao Yuxiao Liao Dan Li Dan Li Xiaolei Zhou Xiaolei Zhou Zhao Peng Zhao Peng Zitong Meng Zitong Meng Rui Liu Wei Yang Wei Yang Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation
description	Background: The previous studies demonstrated that there might be complex and close relationships among leucine supplementation, gut microbiota, and muscle health, which still needs further investigation.Aims: This study aimed to explore the associations of gut microbiota with muscle health after leucine intake.Methods: In this study, 19-month-old male C57BL/6j mice (n = 12/group) were supplemented with ultrapure water, low dose of leucine (500 mg/kg·d), and high dose of leucine (1,250 mg/kg·d) for 12 weeks by oral gavage. The mice fecal samples in each group before and after supplementation were collected for baseline and endpoint gut microbiota analysis by using 16S rDNA amplicon sequencing. Meanwhile, ultrasound measurement, H&E staining, myofiber cross-sectional area (CSA) measurement, and western blotting were performed in the quadriceps subsequently. The pyruvate levels were detected in feces.Results: Improvement in muscle of histology and ultrasonography were observed after both low and high dose of leucine supplementation. High dose of leucine supplementation could promote skeletal muscle health in aging mice via regulating AMPKα/SIRT1/PGC-1α. The richness and diversities of microbiota as well as enriched metabolic pathways were altered after leucine supplementation. Firmicutes-Bacteroidetes ratio was significantly decreased in high-leucine group. Moreover, pyruvate fermentation to propanoate I were negatively associated with differential species and the pyruvate levels were significantly increased in feces after high dose of leucine supplementation.Conclusions: Chronic high dose of leucine supplementation changed gut microbiota composition and increased pyruvate levels in the feces, which possibly provides a novel direction for promoting muscle health in aging mice.
format	article
author	Yuxiao Liao Yuxiao Liao Dan Li Dan Li Xiaolei Zhou Xiaolei Zhou Zhao Peng Zhao Peng Zitong Meng Zitong Meng Rui Liu Wei Yang Wei Yang
author_facet	Yuxiao Liao Yuxiao Liao Dan Li Dan Li Xiaolei Zhou Xiaolei Zhou Zhao Peng Zhao Peng Zitong Meng Zitong Meng Rui Liu Wei Yang Wei Yang
author_sort	Yuxiao Liao
title	Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation
title_short	Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation
title_full	Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation
title_fullStr	Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation
title_full_unstemmed	Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation
title_sort	pyruvate might bridge gut microbiota and muscle health in aging mice after chronic high dose of leucine supplementation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5dd587e2be8f46139cf16d99812f1ac8
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Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation

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