Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain

Abstract Background As the critical tissue of the central nervous system, the brain has been found to be involved in gonad development. Previous studies have suggested that gonadal fate may be affected by the brain. Identifying brain-specific molecular changes that occur during estrodiol-17β (E2) -i...

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Autores principales:	Xufang Shen, Hongwei Yan, Jieming Jiang, Weiyuan Li, Yuyu Xiong, Qi Liu, Ying Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Takifugu rubripes Brain Estradiol-17β Sex differentiation Transcriptome analysis Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/5dda10bead644051b9df9293aef109c0
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spelling	oai:doaj.org-article:5dda10bead644051b9df9293aef109c02021-11-28T12:23:12ZProfile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain10.1186/s12864-021-08158-01471-2164https://doaj.org/article/5dda10bead644051b9df9293aef109c02021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08158-0https://doaj.org/toc/1471-2164Abstract Background As the critical tissue of the central nervous system, the brain has been found to be involved in gonad development. Previous studies have suggested that gonadal fate may be affected by the brain. Identifying brain-specific molecular changes that occur during estrodiol-17β (E2) -induced feminization is crucial to our understanding of the molecular control of sex differentiation by the brains of fish. Results In this study, the differential transcriptomic responses of the Takifugu rubripes larvae brain were compared after E2 treatment for 55 days. Our results showed that 514 genes were differentially expressed between E2-treated-XX (E-XX) and Control-XX (C-XX) T. rubripes, while 362 genes were differentially expressed between E2-treated-XY (E-XY) and Control-XY (C-XY). For example, the expression of cyp19a1b, gnrh1 and pgr was significantly up-regulated, while st, sl, tshβ, prl and pit-1, which belong to the growth hormone/prolactin family, were significantly down-regulated after E2 treatment, in both sexes. The arntl1, bhlbe, nr1d2, per1b, per3, cry1, cipc and ciart genes, which are involved in the circadian rhythm, were also found to be altered. Differentially expressed genes (DEGs), which were identified between E-XX and C-XX, were significantly enriched in neuroactive ligand-receptor interaction, arachidonic acid metabolism, cytokine-cytokine receptor interaction and the calcium signaling pathway. The DEGs that were identified between E-XY and C-XY were significantly enriched in tyrosine metabolism, phenylalanine metabolism, arachidonic acid metabolism and linoleic acid metabolism. Conclusion A number of genes and pathways were identified in the brain of E2-treated T. rubripes larvae by RNA-seq. It provided the opportunity for further study on the possible involvement of networks in the brain-pituitary-gonadal axis in sex differentiation in T. rubripes.Xufang ShenHongwei YanJieming JiangWeiyuan LiYuyu XiongQi LiuYing LiuBMCarticleTakifugu rubripesBrainEstradiol-17βSex differentiationTranscriptome analysisBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Takifugu rubripes Brain Estradiol-17β Sex differentiation Transcriptome analysis Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Takifugu rubripes Brain Estradiol-17β Sex differentiation Transcriptome analysis Biotechnology TP248.13-248.65 Genetics QH426-470 Xufang Shen Hongwei Yan Jieming Jiang Weiyuan Li Yuyu Xiong Qi Liu Ying Liu Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain
description	Abstract Background As the critical tissue of the central nervous system, the brain has been found to be involved in gonad development. Previous studies have suggested that gonadal fate may be affected by the brain. Identifying brain-specific molecular changes that occur during estrodiol-17β (E2) -induced feminization is crucial to our understanding of the molecular control of sex differentiation by the brains of fish. Results In this study, the differential transcriptomic responses of the Takifugu rubripes larvae brain were compared after E2 treatment for 55 days. Our results showed that 514 genes were differentially expressed between E2-treated-XX (E-XX) and Control-XX (C-XX) T. rubripes, while 362 genes were differentially expressed between E2-treated-XY (E-XY) and Control-XY (C-XY). For example, the expression of cyp19a1b, gnrh1 and pgr was significantly up-regulated, while st, sl, tshβ, prl and pit-1, which belong to the growth hormone/prolactin family, were significantly down-regulated after E2 treatment, in both sexes. The arntl1, bhlbe, nr1d2, per1b, per3, cry1, cipc and ciart genes, which are involved in the circadian rhythm, were also found to be altered. Differentially expressed genes (DEGs), which were identified between E-XX and C-XX, were significantly enriched in neuroactive ligand-receptor interaction, arachidonic acid metabolism, cytokine-cytokine receptor interaction and the calcium signaling pathway. The DEGs that were identified between E-XY and C-XY were significantly enriched in tyrosine metabolism, phenylalanine metabolism, arachidonic acid metabolism and linoleic acid metabolism. Conclusion A number of genes and pathways were identified in the brain of E2-treated T. rubripes larvae by RNA-seq. It provided the opportunity for further study on the possible involvement of networks in the brain-pituitary-gonadal axis in sex differentiation in T. rubripes.
format	article
author	Xufang Shen Hongwei Yan Jieming Jiang Weiyuan Li Yuyu Xiong Qi Liu Ying Liu
author_facet	Xufang Shen Hongwei Yan Jieming Jiang Weiyuan Li Yuyu Xiong Qi Liu Ying Liu
author_sort	Xufang Shen
title	Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain
title_short	Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain
title_full	Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain
title_fullStr	Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain
title_full_unstemmed	Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain
title_sort	profile of gene expression changes during estrodiol-17β-induced feminization in the takifugu rubripes brain
publisher	BMC
publishDate	2021
url	https://doaj.org/article/5dda10bead644051b9df9293aef109c0
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Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain

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