Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.

Interactions occurring between malignant cells and the stromal microenvironment heavily influence tumor progression. We investigated whether this cross-talk affects some molecular and functional aspects specifically correlated with the invasive phenotype of breast tumor cells (i.e. adhesion molecule...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cristiana Angelucci, Giuseppe Maulucci, Gina Lama, Gabriella Proietti, Anna Colabianchi, Massimiliano Papi, Alessandro Maiorana, Marco De Spirito, Alessandra Micera, Omar Bijorn Balzamino, Alba Di Leone, Riccardo Masetti, Gigliola Sica
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ddb606713a646948b689df132d00485
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5ddb606713a646948b689df132d00485
record_format	dspace
spelling	oai:doaj.org-article:5ddb606713a646948b689df132d004852021-11-18T08:05:51ZEpithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.1932-620310.1371/journal.pone.0050804https://doaj.org/article/5ddb606713a646948b689df132d004852012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251387/?tool=EBIhttps://doaj.org/toc/1932-6203Interactions occurring between malignant cells and the stromal microenvironment heavily influence tumor progression. We investigated whether this cross-talk affects some molecular and functional aspects specifically correlated with the invasive phenotype of breast tumor cells (i.e. adhesion molecule expression, membrane fluidity, migration) by co-culturing mammary cancer cells exhibiting different degrees of metastatic potential (MDA-MB-231>MCF-7) with fibroblasts isolated from breast healthy skin (normal fibroblasts, NFs) or from breast tumor stroma (cancer-associated fibroblasts, CAFs) in 2D or 3D (nodules) cultures. Confocal immunofluorescence analysis of the epithelial adhesion molecule E-cadherin on frozen nodule sections demonstrated that NFs and CAFs, respectively, induced or inhibited its expression in MCF-7 cells. An increase in the mesenchymal adhesion protein N-cadherin was observed in CAFs, but not in NFs, as a result of the interaction with both kinds of cancer cells. CAFs, in turn, promoted N-cadherin up-regulation in MDA-MB-231 cells and its de novo expression in MCF-7 cells. Beyond promotion of "cadherin switching", another sign of the CAF-triggered epithelial-mesenchymal transition (EMT) was the induction of vimentin expression in MCF-7 cells. Plasma membrane labeling of monolayer cultures with the fluorescent probe Laurdan showed an enhancement of the membrane fluidity in cancer cells co-cultured with NFs or CAFs. An increase in lipid packing density of fibroblast membranes was promoted by MCF-7 cells. Time-lapsed cell tracking analysis of mammary cancer cells co-cultured with NFs or CAFs revealed an enhancement of tumor cell migration velocity, even with a marked increase in the directness induced by CAFs.Our results demonstrate a reciprocal influence of mammary cancer and fibroblasts on various adhesiveness/invasiveness features. Notably, CAFs' ability to promote EMT, reduction of cell adhesion, increase in membrane fluidity, and migration velocity and directness in mammary cancer cells can be viewed as an overall progression- and invasion-promoting effect.Cristiana AngelucciGiuseppe MaulucciGina LamaGabriella ProiettiAnna ColabianchiMassimiliano PapiAlessandro MaioranaMarco De SpiritoAlessandra MiceraOmar Bijorn BalzaminoAlba Di LeoneRiccardo MasettiGigliola SicaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e50804 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristiana Angelucci Giuseppe Maulucci Gina Lama Gabriella Proietti Anna Colabianchi Massimiliano Papi Alessandro Maiorana Marco De Spirito Alessandra Micera Omar Bijorn Balzamino Alba Di Leone Riccardo Masetti Gigliola Sica Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
description	Interactions occurring between malignant cells and the stromal microenvironment heavily influence tumor progression. We investigated whether this cross-talk affects some molecular and functional aspects specifically correlated with the invasive phenotype of breast tumor cells (i.e. adhesion molecule expression, membrane fluidity, migration) by co-culturing mammary cancer cells exhibiting different degrees of metastatic potential (MDA-MB-231>MCF-7) with fibroblasts isolated from breast healthy skin (normal fibroblasts, NFs) or from breast tumor stroma (cancer-associated fibroblasts, CAFs) in 2D or 3D (nodules) cultures. Confocal immunofluorescence analysis of the epithelial adhesion molecule E-cadherin on frozen nodule sections demonstrated that NFs and CAFs, respectively, induced or inhibited its expression in MCF-7 cells. An increase in the mesenchymal adhesion protein N-cadherin was observed in CAFs, but not in NFs, as a result of the interaction with both kinds of cancer cells. CAFs, in turn, promoted N-cadherin up-regulation in MDA-MB-231 cells and its de novo expression in MCF-7 cells. Beyond promotion of "cadherin switching", another sign of the CAF-triggered epithelial-mesenchymal transition (EMT) was the induction of vimentin expression in MCF-7 cells. Plasma membrane labeling of monolayer cultures with the fluorescent probe Laurdan showed an enhancement of the membrane fluidity in cancer cells co-cultured with NFs or CAFs. An increase in lipid packing density of fibroblast membranes was promoted by MCF-7 cells. Time-lapsed cell tracking analysis of mammary cancer cells co-cultured with NFs or CAFs revealed an enhancement of tumor cell migration velocity, even with a marked increase in the directness induced by CAFs.Our results demonstrate a reciprocal influence of mammary cancer and fibroblasts on various adhesiveness/invasiveness features. Notably, CAFs' ability to promote EMT, reduction of cell adhesion, increase in membrane fluidity, and migration velocity and directness in mammary cancer cells can be viewed as an overall progression- and invasion-promoting effect.
format	article
author	Cristiana Angelucci Giuseppe Maulucci Gina Lama Gabriella Proietti Anna Colabianchi Massimiliano Papi Alessandro Maiorana Marco De Spirito Alessandra Micera Omar Bijorn Balzamino Alba Di Leone Riccardo Masetti Gigliola Sica
author_facet	Cristiana Angelucci Giuseppe Maulucci Gina Lama Gabriella Proietti Anna Colabianchi Massimiliano Papi Alessandro Maiorana Marco De Spirito Alessandra Micera Omar Bijorn Balzamino Alba Di Leone Riccardo Masetti Gigliola Sica
author_sort	Cristiana Angelucci
title	Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
title_short	Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
title_full	Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
title_fullStr	Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
title_full_unstemmed	Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
title_sort	epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5ddb606713a646948b689df132d00485
work_keys_str_mv	AT cristianaangelucci epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT giuseppemaulucci epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT ginalama epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT gabriellaproietti epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT annacolabianchi epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT massimilianopapi epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT alessandromaiorana epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT marcodespirito epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT alessandramicera epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT omarbijornbalzamino epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT albadileone epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT riccardomasetti epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness AT gigliolasica epithelialstromalinteractionsinhumanbreastcancereffectsonadhesionplasmamembranefluidityandmigrationspeedanddirectness
_version_	1718422230365372416

Epithelial-stromal interactions in human breast cancer: effects on adhesion, plasma membrane fluidity and migration speed and directness.

Ejemplares similares