Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.

<h4>Background</h4>The oxidation of proteins by endogenously generated free radicals causes structural modifications in the molecules that lead to generation of neo-antigenic epitopes that have implications in various autoimmune disorders, including rheumatoid arthritis (RA). Collagen in...

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Autores principales:	Uzma Shahab, Saheem Ahmad, Moinuddin, Kiran Dixit, Safia Habib, Khursheed Alam, Asif Ali
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ddd0c5ffb3c4c1abaefd8f4d17d50a3
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spelling	oai:doaj.org-article:5ddd0c5ffb3c4c1abaefd8f4d17d50a32021-11-18T07:28:52ZHydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.1932-620310.1371/journal.pone.0031199https://doaj.org/article/5ddd0c5ffb3c4c1abaefd8f4d17d50a32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22319617/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The oxidation of proteins by endogenously generated free radicals causes structural modifications in the molecules that lead to generation of neo-antigenic epitopes that have implications in various autoimmune disorders, including rheumatoid arthritis (RA). Collagen induced arthritis (CIA) in rodents (rats and mice) is an accepted experimental model for RA.<h4>Methodology/principal findings</h4>Hydroxyl radicals were generated by the Fenton reaction. Collagen type II (CII) was modified by •OH radical (CII-OH) and analysed by ultraviolet-visible (UV-VIS), fluorescence and circular dichroism (CD) spectroscopy. The immunogenicity of native and modified CII was checked in female Lewis rats and specificity of the induced antibodies was ascertained by enzyme linked immunosorbent assay (ELISA). The extent of CIA was evaluated by visual inspection. We also estimated the oxidative and inflammatory markers in the sera of immunized rats. A slight change in the triple helical structure of CII as well as fragmentation was observed after hydroxyl radical modification. The modified CII was found to be highly arthritogenic and immunogenic as compared to the native form. The CII-OH immunized rats exhibited increased oxidative stress and inflammation as compared to the CII immunized rats in the control group.<h4>Conclusions/significance</h4>Neo-antigenic epitopes were generated on (•)OH modified CII which rendered it highly immunogenic and arthritogenic as compared to the unmodified form. Since the rodent CIA model shares many features with human RA, these results illuminate the role of free radicals in human RA.Uzma ShahabSaheem AhmadMoinuddinKiran DixitSafia HabibKhursheed AlamAsif AliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31199 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Uzma Shahab Saheem Ahmad Moinuddin Kiran Dixit Safia Habib Khursheed Alam Asif Ali Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.
description	<h4>Background</h4>The oxidation of proteins by endogenously generated free radicals causes structural modifications in the molecules that lead to generation of neo-antigenic epitopes that have implications in various autoimmune disorders, including rheumatoid arthritis (RA). Collagen induced arthritis (CIA) in rodents (rats and mice) is an accepted experimental model for RA.<h4>Methodology/principal findings</h4>Hydroxyl radicals were generated by the Fenton reaction. Collagen type II (CII) was modified by •OH radical (CII-OH) and analysed by ultraviolet-visible (UV-VIS), fluorescence and circular dichroism (CD) spectroscopy. The immunogenicity of native and modified CII was checked in female Lewis rats and specificity of the induced antibodies was ascertained by enzyme linked immunosorbent assay (ELISA). The extent of CIA was evaluated by visual inspection. We also estimated the oxidative and inflammatory markers in the sera of immunized rats. A slight change in the triple helical structure of CII as well as fragmentation was observed after hydroxyl radical modification. The modified CII was found to be highly arthritogenic and immunogenic as compared to the native form. The CII-OH immunized rats exhibited increased oxidative stress and inflammation as compared to the CII immunized rats in the control group.<h4>Conclusions/significance</h4>Neo-antigenic epitopes were generated on (•)OH modified CII which rendered it highly immunogenic and arthritogenic as compared to the unmodified form. Since the rodent CIA model shares many features with human RA, these results illuminate the role of free radicals in human RA.
format	article
author	Uzma Shahab Saheem Ahmad Moinuddin Kiran Dixit Safia Habib Khursheed Alam Asif Ali
author_facet	Uzma Shahab Saheem Ahmad Moinuddin Kiran Dixit Safia Habib Khursheed Alam Asif Ali
author_sort	Uzma Shahab
title	Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.
title_short	Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.
title_full	Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.
title_fullStr	Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.
title_full_unstemmed	Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.
title_sort	hydroxyl radical modification of collagen type ii increases its arthritogenicity and immunogenicity.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5ddd0c5ffb3c4c1abaefd8f4d17d50a3
work_keys_str_mv	AT uzmashahab hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity AT saheemahmad hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity AT moinuddin hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity AT kirandixit hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity AT safiahabib hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity AT khursheedalam hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity AT asifali hydroxylradicalmodificationofcollagentypeiiincreasesitsarthritogenicityandimmunogenicity
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Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity.

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