Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.

Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from plants Ochrosia elliptica, showed anticancer activity. A previous study demonstrated that ellipticine exhibited a reduction of the proliferation and self-renewal ability of ALDH1A1-positive breast cancer stem cells; how...

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Autores principales:	Adam Hermawan, Herwandhani Putri
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	ellipticine anticancer bioinformatics breast cancer stem cells cd59 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/5ddee6b239ac4ad7a3f5e9bff06aa1d8
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spelling	oai:doaj.org-article:5ddee6b239ac4ad7a3f5e9bff06aa1d82021-11-15T06:08:47ZIntegrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.2338-94272338-948610.22146/ijp.1126https://doaj.org/article/5ddee6b239ac4ad7a3f5e9bff06aa1d82021-02-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1126https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from plants Ochrosia elliptica, showed anticancer activity. A previous study demonstrated that ellipticine exhibited a reduction of the proliferation and self-renewal ability of ALDH1A1-positive breast cancer stem cells; however, the mechanisms have not been fully clarified. In this study, we aimed to identify the potential target and mechanism of ellipticine in breast cancer stem cells with a bioinformatics approach. Gene expression profiles predicting sensitivity and resistance of tumor cells to ellipticine were determined by microarray-based mRNA expressions from COMPARE. Gene list related to breast cancer stem cells was obtained from Pubmed with keywords "breast cancer stem cells". Protein-protein interaction (PPI) network construction, Gene Ontology (GO) and KEGG pathway enrichment analysis were carried out by STRING-DB. The genetic alterations of hub genes were analysed using cBioPortal. The prognostic value of the genes was evaluated using Kaplan-Meier survival curves. A Venn diagram of COMPARE microarray data and the gene list from PubMed generates two genes that are regulated by ellipticine and are related to breast cancer stem cells, namely XRCC5 and CD59. Ellipticine might target complement cascade and DNA repair mechanism in breast cancer stem cells. Ellipticine might target complement cascade and DNA repair mechanism in breast cancer stem cells. More importantly, CD59 is a potential biomarker to evaluate ellipticine bioactivity in BCSCs. Results of this study could be useful for elucidation of ellipticine molecular mechanism as well as for targeting BCSCs.Adam HermawanHerwandhani PutriUniversitas Gadjah Madaarticleellipticineanticancerbioinformaticsbreast cancer stem cellscd59Pharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 64-73 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ellipticine anticancer bioinformatics breast cancer stem cells cd59 Pharmacy and materia medica RS1-441
spellingShingle	ellipticine anticancer bioinformatics breast cancer stem cells cd59 Pharmacy and materia medica RS1-441 Adam Hermawan Herwandhani Putri Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.
description	Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from plants Ochrosia elliptica, showed anticancer activity. A previous study demonstrated that ellipticine exhibited a reduction of the proliferation and self-renewal ability of ALDH1A1-positive breast cancer stem cells; however, the mechanisms have not been fully clarified. In this study, we aimed to identify the potential target and mechanism of ellipticine in breast cancer stem cells with a bioinformatics approach. Gene expression profiles predicting sensitivity and resistance of tumor cells to ellipticine were determined by microarray-based mRNA expressions from COMPARE. Gene list related to breast cancer stem cells was obtained from Pubmed with keywords "breast cancer stem cells". Protein-protein interaction (PPI) network construction, Gene Ontology (GO) and KEGG pathway enrichment analysis were carried out by STRING-DB. The genetic alterations of hub genes were analysed using cBioPortal. The prognostic value of the genes was evaluated using Kaplan-Meier survival curves. A Venn diagram of COMPARE microarray data and the gene list from PubMed generates two genes that are regulated by ellipticine and are related to breast cancer stem cells, namely XRCC5 and CD59. Ellipticine might target complement cascade and DNA repair mechanism in breast cancer stem cells. Ellipticine might target complement cascade and DNA repair mechanism in breast cancer stem cells. More importantly, CD59 is a potential biomarker to evaluate ellipticine bioactivity in BCSCs. Results of this study could be useful for elucidation of ellipticine molecular mechanism as well as for targeting BCSCs.
format	article
author	Adam Hermawan Herwandhani Putri
author_facet	Adam Hermawan Herwandhani Putri
author_sort	Adam Hermawan
title	Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.
title_short	Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.
title_full	Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.
title_fullStr	Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.
title_full_unstemmed	Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.
title_sort	integrative bioinformatics analysis reveals possible target and mechanism of ellipticine against breast cancer stem cells.
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/5ddee6b239ac4ad7a3f5e9bff06aa1d8
work_keys_str_mv	AT adamhermawan integrativebioinformaticsanalysisrevealspossibletargetandmechanismofellipticineagainstbreastcancerstemcells AT herwandhaniputri integrativebioinformaticsanalysisrevealspossibletargetandmechanismofellipticineagainstbreastcancerstemcells
_version_	1718428554638655488

Integrative Bioinformatics Analysis Reveals Possible Target and Mechanism of Ellipticine Against Breast Cancer Stem Cells.

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