Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells

Tick-borne encephalitis virus (TBEV), a member of the <i>Flaviviridae</i> family, <i>Flavivirus</i> genus, is responsible for neurological symptoms that may cause permanent disability or death. With an incidence on the rise, it is the major arbovirus affecting humans in Centr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mazigh Fares, Kamila Gorna, Noémie Berry, Marielle Cochet-Bernoin, François Piumi, Odile Blanchet, Nadia Haddad, Jennifer Richardson, Muriel Coulpier
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	central nervous system flavivirus neuronal death pathological modeling tick-borne encephalitis virus TNF family members Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5de4f915746941d6940647ff896ac8da
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5de4f915746941d6940647ff896ac8da
record_format	dspace
spelling	oai:doaj.org-article:5de4f915746941d6940647ff896ac8da2021-11-25T19:13:49ZTranscriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells10.3390/v131122551999-4915https://doaj.org/article/5de4f915746941d6940647ff896ac8da2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2255https://doaj.org/toc/1999-4915Tick-borne encephalitis virus (TBEV), a member of the <i>Flaviviridae</i> family, <i>Flavivirus</i> genus, is responsible for neurological symptoms that may cause permanent disability or death. With an incidence on the rise, it is the major arbovirus affecting humans in Central/Northern Europe and North-Eastern Asia. Neuronal death is a critical feature of TBEV infection, yet little is known about the type of death and the molecular mechanisms involved. In this study, we used a recently established pathological model of TBEV infection based on human neuronal/glial cells differentiated from fetal neural progenitors and transcriptomic approaches to tackle this question. We confirmed the occurrence of apoptotic death in these cultures and further showed that genes involved in pyroptotic death were up-regulated, suggesting that this type of death also occurs in TBEV-infected human brain cells. On the contrary, no up-regulation of major autophagic genes was found. Furthermore, we demonstrated an up-regulation of a cluster of genes belonging to the extrinsic apoptotic pathway and revealed the cellular types expressing them. Our results suggest that neuronal death occurs by multiple mechanisms in TBEV-infected human neuronal/glial cells, thus providing a first insight into the molecular pathways that may be involved in neuronal death when the human brain is infected by TBEV.Mazigh FaresKamila GornaNoémie BerryMarielle Cochet-BernoinFrançois PiumiOdile BlanchetNadia HaddadJennifer RichardsonMuriel CoulpierMDPI AGarticlecentral nervous systemflavivirusneuronal deathpathological modelingtick-borne encephalitis virusTNF family membersMicrobiologyQR1-502ENViruses, Vol 13, Iss 2255, p 2255 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	central nervous system flavivirus neuronal death pathological modeling tick-borne encephalitis virus TNF family members Microbiology QR1-502
spellingShingle	central nervous system flavivirus neuronal death pathological modeling tick-borne encephalitis virus TNF family members Microbiology QR1-502 Mazigh Fares Kamila Gorna Noémie Berry Marielle Cochet-Bernoin François Piumi Odile Blanchet Nadia Haddad Jennifer Richardson Muriel Coulpier Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells
description	Tick-borne encephalitis virus (TBEV), a member of the <i>Flaviviridae</i> family, <i>Flavivirus</i> genus, is responsible for neurological symptoms that may cause permanent disability or death. With an incidence on the rise, it is the major arbovirus affecting humans in Central/Northern Europe and North-Eastern Asia. Neuronal death is a critical feature of TBEV infection, yet little is known about the type of death and the molecular mechanisms involved. In this study, we used a recently established pathological model of TBEV infection based on human neuronal/glial cells differentiated from fetal neural progenitors and transcriptomic approaches to tackle this question. We confirmed the occurrence of apoptotic death in these cultures and further showed that genes involved in pyroptotic death were up-regulated, suggesting that this type of death also occurs in TBEV-infected human brain cells. On the contrary, no up-regulation of major autophagic genes was found. Furthermore, we demonstrated an up-regulation of a cluster of genes belonging to the extrinsic apoptotic pathway and revealed the cellular types expressing them. Our results suggest that neuronal death occurs by multiple mechanisms in TBEV-infected human neuronal/glial cells, thus providing a first insight into the molecular pathways that may be involved in neuronal death when the human brain is infected by TBEV.
format	article
author	Mazigh Fares Kamila Gorna Noémie Berry Marielle Cochet-Bernoin François Piumi Odile Blanchet Nadia Haddad Jennifer Richardson Muriel Coulpier
author_facet	Mazigh Fares Kamila Gorna Noémie Berry Marielle Cochet-Bernoin François Piumi Odile Blanchet Nadia Haddad Jennifer Richardson Muriel Coulpier
author_sort	Mazigh Fares
title	Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells
title_short	Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells
title_full	Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells
title_fullStr	Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells
title_full_unstemmed	Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells
title_sort	transcriptomic studies suggest a coincident role for apoptosis and pyroptosis but not for autophagic neuronal death in tbev-infected human neuronal/glial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5de4f915746941d6940647ff896ac8da
work_keys_str_mv	AT mazighfares transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT kamilagorna transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT noemieberry transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT mariellecochetbernoin transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT francoispiumi transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT odileblanchet transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT nadiahaddad transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT jenniferrichardson transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells AT murielcoulpier transcriptomicstudiessuggestacoincidentroleforapoptosisandpyroptosisbutnotforautophagicneuronaldeathintbevinfectedhumanneuronalglialcells
_version_	1718410158158118912

Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells

Ejemplares similares