Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution

The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-ad...

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Main Authors:	Kaiming Zhang, Shanshan Li, Kalli Kappel, Grigore Pintilie, Zhaoming Su, Tung-Chung Mou, Michael F. Schmid, Rhiju Das, Wah Chiu
Format:	article
Language:	EN
Published:	Nature Portfolio 2019
Subjects:	Science Q
Online Access:	https://doaj.org/article/5de6b22e3c6a4ad29f1e5dabe8c50f5e
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spelling	oai:doaj.org-article:5de6b22e3c6a4ad29f1e5dabe8c50f5e2021-12-02T14:35:30ZCryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution10.1038/s41467-019-13494-72041-1723https://doaj.org/article/5de6b22e3c6a4ad29f1e5dabe8c50f5e2019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13494-7https://doaj.org/toc/2041-1723The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-adenosylmethionine.Kaiming ZhangShanshan LiKalli KappelGrigore PintilieZhaoming SuTung-Chung MouMichael F. SchmidRhiju DasWah ChiuNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kaiming Zhang Shanshan Li Kalli Kappel Grigore Pintilie Zhaoming Su Tung-Chung Mou Michael F. Schmid Rhiju Das Wah Chiu Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution
description	The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-adenosylmethionine.
format	article
author	Kaiming Zhang Shanshan Li Kalli Kappel Grigore Pintilie Zhaoming Su Tung-Chung Mou Michael F. Schmid Rhiju Das Wah Chiu
author_facet	Kaiming Zhang Shanshan Li Kalli Kappel Grigore Pintilie Zhaoming Su Tung-Chung Mou Michael F. Schmid Rhiju Das Wah Chiu
author_sort	Kaiming Zhang
title	Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution
title_short	Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution
title_full	Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution
title_fullStr	Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution
title_full_unstemmed	Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution
title_sort	cryo-em structure of a 40 kda sam-iv riboswitch rna at 3.7 å resolution
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5de6b22e3c6a4ad29f1e5dabe8c50f5e
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_version_	1718391131581972480

Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution

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