Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress

Abstract The development of insects is critically affected by temperature, which therefore plays an important role in the control of stored grain pests. Extreme temperature stress conditions lead to biological responses in mites, such as the synthesis of heat shock proteins. Tyrophagus putrescentiae...

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Autores principales:	Jing Wang, Sheng-quan Que, Xinyu Liu, Mengru Jin, Tian-Rong Xin, Zhi-wen Zou, Bin Xia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5de7977323e840f8901bd48b55d57133
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spelling	oai:doaj.org-article:5de7977323e840f8901bd48b55d571332021-12-02T15:57:21ZCharacteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress10.1038/s41598-021-91206-22045-2322https://doaj.org/article/5de7977323e840f8901bd48b55d571332021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91206-2https://doaj.org/toc/2045-2322Abstract The development of insects is critically affected by temperature, which therefore plays an important role in the control of stored grain pests. Extreme temperature stress conditions lead to biological responses in mites, such as the synthesis of heat shock proteins. Tyrophagus putrescentiae (Tp) is a pest mite in stored grain that has negative effects on both economy and health. Since T. putrescentiae population dynamics are strongly influenced by temperature, in the present study we have cloned the cDNA of HSP70 and HSP90 (referred to as TpHSP70-1, TpHSP70-2 and TpHSP90) and determined their expression by fluorescence real time quantitative PCR. TpHSP70 and TpHSP90 showed high homology with similar genes in other species and the open reading frames of TpHSP70-1, TpHSP70-2 and TpHSP90 encoded proteins of 665, 661 and 718 amino acid residues, respectively. Under thermal stress, expression of TpHsp70-1 and TpHsp90 was up-regulated at higher temperatures, suggesting their role in the defense against thermal stress.Jing WangSheng-quan QueXinyu LiuMengru JinTian-Rong XinZhi-wen ZouBin XiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Wang Sheng-quan Que Xinyu Liu Mengru Jin Tian-Rong Xin Zhi-wen Zou Bin Xia Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress
description	Abstract The development of insects is critically affected by temperature, which therefore plays an important role in the control of stored grain pests. Extreme temperature stress conditions lead to biological responses in mites, such as the synthesis of heat shock proteins. Tyrophagus putrescentiae (Tp) is a pest mite in stored grain that has negative effects on both economy and health. Since T. putrescentiae population dynamics are strongly influenced by temperature, in the present study we have cloned the cDNA of HSP70 and HSP90 (referred to as TpHSP70-1, TpHSP70-2 and TpHSP90) and determined their expression by fluorescence real time quantitative PCR. TpHSP70 and TpHSP90 showed high homology with similar genes in other species and the open reading frames of TpHSP70-1, TpHSP70-2 and TpHSP90 encoded proteins of 665, 661 and 718 amino acid residues, respectively. Under thermal stress, expression of TpHsp70-1 and TpHsp90 was up-regulated at higher temperatures, suggesting their role in the defense against thermal stress.
format	article
author	Jing Wang Sheng-quan Que Xinyu Liu Mengru Jin Tian-Rong Xin Zhi-wen Zou Bin Xia
author_facet	Jing Wang Sheng-quan Que Xinyu Liu Mengru Jin Tian-Rong Xin Zhi-wen Zou Bin Xia
author_sort	Jing Wang
title	Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress
title_short	Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress
title_full	Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress
title_fullStr	Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress
title_full_unstemmed	Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress
title_sort	characteristic and expression of hsp70 and hsp90 genes from tyrophagus putrescentiae and their response to thermal stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5de7977323e840f8901bd48b55d57133
work_keys_str_mv	AT jingwang characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress AT shengquanque characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress AT xinyuliu characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress AT mengrujin characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress AT tianrongxin characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress AT zhiwenzou characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress AT binxia characteristicandexpressionofhsp70andhsp90genesfromtyrophagusputrescentiaeandtheirresponsetothermalstress
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Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress

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