Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In...

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Autores principales:	WINDA AYU SYAFITRI, FITRIA NINGSIH, PUTRI PRATIWI SETYANINGSIH, MAZYTHA KINANTI RACHMANIA, DHIAN CHITRA AYU FITRIA SARI, SHUHEI YABE, AKIRA YOKOTA, ARIYANTI OETARI, WELLYZAR SJAMSURIDZAL
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	amylolytic activity, geothermal area, soil, thermophilic actinobacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5de7b6b06489489a89a37f7545d476f5
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spelling	oai:doaj.org-article:5de7b6b06489489a89a37f7545d476f52021-11-21T21:42:27ZScreening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia1412-033X2085-472210.13057/biodiv/d200720https://doaj.org/article/5de7b6b06489489a89a37f7545d476f52019-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4030https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45oC. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60oC. Fifteen of the 17 isolates showed amylolytic activity at 45oC, 13 showed such activity at 50oC, and four showed activity at 55oC. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60oC. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.WINDA AYU SYAFITRIFITRIA NINGSIHPUTRI PRATIWI SETYANINGSIHMAZYTHA KINANTI RACHMANIADHIAN CHITRA AYU FITRIA SARISHUHEI YABEAKIRA YOKOTAARIYANTI OETARIWELLYZAR SJAMSURIDZALMBI & UNS Soloarticleamylolytic activity, geothermal area, soil, thermophilic actinobacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	amylolytic activity, geothermal area, soil, thermophilic actinobacteria Biology (General) QH301-705.5
spellingShingle	amylolytic activity, geothermal area, soil, thermophilic actinobacteria Biology (General) QH301-705.5 WINDA AYU SYAFITRI FITRIA NINGSIH PUTRI PRATIWI SETYANINGSIH MAZYTHA KINANTI RACHMANIA DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI WELLYZAR SJAMSURIDZAL Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia
description	Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45oC. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60oC. Fifteen of the 17 isolates showed amylolytic activity at 45oC, 13 showed such activity at 50oC, and four showed activity at 55oC. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60oC. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.
format	article
author	WINDA AYU SYAFITRI FITRIA NINGSIH PUTRI PRATIWI SETYANINGSIH MAZYTHA KINANTI RACHMANIA DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI WELLYZAR SJAMSURIDZAL
author_facet	WINDA AYU SYAFITRI FITRIA NINGSIH PUTRI PRATIWI SETYANINGSIH MAZYTHA KINANTI RACHMANIA DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI WELLYZAR SJAMSURIDZAL
author_sort	WINDA AYU SYAFITRI
title	Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia
title_short	Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia
title_full	Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia
title_fullStr	Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia
title_full_unstemmed	Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia
title_sort	screening for amylolytic activity and characterization of thermophilic actinobacteria isolated from a geothermal area in west java, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/5de7b6b06489489a89a37f7545d476f5
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Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

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