Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice

Inhibin (INH) and anti-Müllerian hormone (AMH) are essential in ovarian folliculogenesis and play an inhibitory role in mammalian fertility. However, the interactive effect of INH and AMH on the animal reproduction remains unknown. This study aimed to determine the possible interaction and synergy b...

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Autores principales:	Xue Yu, Tong Qiao, Liping Hua, Shuanghang Liu, Xinzhe Zhao, Ce Lv, Xuhong Zhao, Jing Wang, Li Han, Liguo Yang, Aixin Liang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	INHA AMH granulosa cells litter size mice Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5df317bc87c542c18de03faa7b613243
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spelling	oai:doaj.org-article:5df317bc87c542c18de03faa7b6132432021-11-08T10:09:13ZSynergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice2297-176910.3389/fvets.2021.747619https://doaj.org/article/5df317bc87c542c18de03faa7b6132432021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.747619/fullhttps://doaj.org/toc/2297-1769Inhibin (INH) and anti-Müllerian hormone (AMH) are essential in ovarian folliculogenesis and play an inhibitory role in mammalian fertility. However, the interactive effect of INH and AMH on the animal reproduction remains unknown. This study aimed to determine the possible interaction and synergy between INH and AMH in steroidogenesis by primary granulosa cells, and investigate their synergistic effect on fertility in mice. In in vitro granulosa cell culture system, we found that the treatment of either INHA or AMH had no significant effect on basal estradiol and progesterone production, whereas both significantly attenuated FSH-induced steroid hormone secretion. Importantly, combined treatment with INHA and AMH showed additive inhibitory effect on FSH-induced estradiol and progesterone production, accompanying a significant downregulation in the expression of FSH-stimulated CYP19A1, HSD3B, CYP11A1, StAR transcripts. The interrelationship of INH and AMH combinations was further investigated through active immune neutralization strategy. Female mice were immunized against INH and AMH eukaryotic expression plasmids, and the litter size was recorded after successfully mating. We observed that both INH and AMH plasmids were able to induce either anti-AMH or anti-INH antibodies in the immunized mice. In comparison with the control group, co-immunization with INH and AMH plasmids induced higher levels of estradiol, resulting in more litter size. Moreover, there was no significant difference on the offspring's weight between each group. Collectively, the results of the present study suggest that INH and AMH have synergistic effect in regulating steroidogenesis and the litter size in mice.Xue YuXue YuTong QiaoLiping HuaShuanghang LiuXinzhe ZhaoCe LvXuhong ZhaoJing WangLi HanLiguo YangAixin LiangFrontiers Media S.A.articleINHAAMHgranulosa cellslitter sizemiceVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	INHA AMH granulosa cells litter size mice Veterinary medicine SF600-1100
spellingShingle	INHA AMH granulosa cells litter size mice Veterinary medicine SF600-1100 Xue Yu Xue Yu Tong Qiao Liping Hua Shuanghang Liu Xinzhe Zhao Ce Lv Xuhong Zhao Jing Wang Li Han Liguo Yang Aixin Liang Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
description	Inhibin (INH) and anti-Müllerian hormone (AMH) are essential in ovarian folliculogenesis and play an inhibitory role in mammalian fertility. However, the interactive effect of INH and AMH on the animal reproduction remains unknown. This study aimed to determine the possible interaction and synergy between INH and AMH in steroidogenesis by primary granulosa cells, and investigate their synergistic effect on fertility in mice. In in vitro granulosa cell culture system, we found that the treatment of either INHA or AMH had no significant effect on basal estradiol and progesterone production, whereas both significantly attenuated FSH-induced steroid hormone secretion. Importantly, combined treatment with INHA and AMH showed additive inhibitory effect on FSH-induced estradiol and progesterone production, accompanying a significant downregulation in the expression of FSH-stimulated CYP19A1, HSD3B, CYP11A1, StAR transcripts. The interrelationship of INH and AMH combinations was further investigated through active immune neutralization strategy. Female mice were immunized against INH and AMH eukaryotic expression plasmids, and the litter size was recorded after successfully mating. We observed that both INH and AMH plasmids were able to induce either anti-AMH or anti-INH antibodies in the immunized mice. In comparison with the control group, co-immunization with INH and AMH plasmids induced higher levels of estradiol, resulting in more litter size. Moreover, there was no significant difference on the offspring's weight between each group. Collectively, the results of the present study suggest that INH and AMH have synergistic effect in regulating steroidogenesis and the litter size in mice.
format	article
author	Xue Yu Xue Yu Tong Qiao Liping Hua Shuanghang Liu Xinzhe Zhao Ce Lv Xuhong Zhao Jing Wang Li Han Liguo Yang Aixin Liang
author_facet	Xue Yu Xue Yu Tong Qiao Liping Hua Shuanghang Liu Xinzhe Zhao Ce Lv Xuhong Zhao Jing Wang Li Han Liguo Yang Aixin Liang
author_sort	Xue Yu
title	Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_short	Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_full	Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_fullStr	Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_full_unstemmed	Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_sort	synergistic regulatory effect of inhibin and anti-müllerian hormone on fertility of mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5df317bc87c542c18de03faa7b613243
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Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice

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