MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)

Nasopharyngeal cancer is a rare cancer type, but with a low five-year survival rate. Dysregulation of pyrroline-5-carboxylate reductase 1 (PYCR1) and microRNA hsa-miR-150-5p is involved in the development of various cancers. However, the molecular mechanism of the hsa-miR-150-5p-PYCR1 axis in nasoph...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhiqun Li, Xiaoliu Zhou, Jiajun Huang, Zhencai Xu, Chengliang Xing, Junwei Yang, Xuejun Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	hsa-mir-150-5p pycr1 nasopharyngeal cancer Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5df6ed47594d43afa7092045f621e1df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5df6ed47594d43afa7092045f621e1df
record_format	dspace
spelling	oai:doaj.org-article:5df6ed47594d43afa7092045f621e1df2021-11-04T15:51:54ZMicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)2165-59792165-598710.1080/21655979.2021.1995102https://doaj.org/article/5df6ed47594d43afa7092045f621e1df2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1995102https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Nasopharyngeal cancer is a rare cancer type, but with a low five-year survival rate. Dysregulation of pyrroline-5-carboxylate reductase 1 (PYCR1) and microRNA hsa-miR-150-5p is involved in the development of various cancers. However, the molecular mechanism of the hsa-miR-150-5p-PYCR1 axis in nasopharyngeal cancer remains unclear. To identify the mechanism of the hsa-miR-150-5p-PYCR1 axis, the expression of hsa-miR-150-5p and PYCR1 in nasopharyngeal cancer tissues and cells was first measured by reverse transcription quantitative polymerase chain reaction. The luciferase and RNA pull-down assays were used to confirm the interaction between hsa-miR-150-5p and PYCR1. The overexpression of hsa-miR-150-5p and PYCR1 was detected by cell viability, proliferation, western blotting, migration, and invasion in nasopharyngeal cancer cells. The expression levels of hsa-miR-150-5p was reduced in the nasopharyngeal cancer tissues and cells and were negatively correlated with the PYCR1 levels. The upregulation of hsa-miR-150-5p significantly repressed cell growth and promoted apoptosis. However, the upregulation of PYCR1 expression significantly promoted nasopharyngeal carcinogenesis, which could abolish the inhibitory effect of hsa-miR-150-5p. In conclusion, we clarified that hsa-miR-150-5p attenuated nasopharyngeal carcinogenesis by reducing the PYCR1 expression levels. This provides a new perspective of nasopharyngeal cancer involving both hsa-miR-150-5p and PYCR1 for the treatment of nasopharyngeal cancer.Zhiqun LiXiaoliu ZhouJiajun HuangZhencai XuChengliang XingJunwei YangXuejun ZhouTaylor & Francis Grouparticlehsa-mir-150-5ppycr1nasopharyngeal cancerBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hsa-mir-150-5p pycr1 nasopharyngeal cancer Biotechnology TP248.13-248.65
spellingShingle	hsa-mir-150-5p pycr1 nasopharyngeal cancer Biotechnology TP248.13-248.65 Zhiqun Li Xiaoliu Zhou Jiajun Huang Zhencai Xu Chengliang Xing Junwei Yang Xuejun Zhou MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
description	Nasopharyngeal cancer is a rare cancer type, but with a low five-year survival rate. Dysregulation of pyrroline-5-carboxylate reductase 1 (PYCR1) and microRNA hsa-miR-150-5p is involved in the development of various cancers. However, the molecular mechanism of the hsa-miR-150-5p-PYCR1 axis in nasopharyngeal cancer remains unclear. To identify the mechanism of the hsa-miR-150-5p-PYCR1 axis, the expression of hsa-miR-150-5p and PYCR1 in nasopharyngeal cancer tissues and cells was first measured by reverse transcription quantitative polymerase chain reaction. The luciferase and RNA pull-down assays were used to confirm the interaction between hsa-miR-150-5p and PYCR1. The overexpression of hsa-miR-150-5p and PYCR1 was detected by cell viability, proliferation, western blotting, migration, and invasion in nasopharyngeal cancer cells. The expression levels of hsa-miR-150-5p was reduced in the nasopharyngeal cancer tissues and cells and were negatively correlated with the PYCR1 levels. The upregulation of hsa-miR-150-5p significantly repressed cell growth and promoted apoptosis. However, the upregulation of PYCR1 expression significantly promoted nasopharyngeal carcinogenesis, which could abolish the inhibitory effect of hsa-miR-150-5p. In conclusion, we clarified that hsa-miR-150-5p attenuated nasopharyngeal carcinogenesis by reducing the PYCR1 expression levels. This provides a new perspective of nasopharyngeal cancer involving both hsa-miR-150-5p and PYCR1 for the treatment of nasopharyngeal cancer.
format	article
author	Zhiqun Li Xiaoliu Zhou Jiajun Huang Zhencai Xu Chengliang Xing Junwei Yang Xuejun Zhou
author_facet	Zhiqun Li Xiaoliu Zhou Jiajun Huang Zhencai Xu Chengliang Xing Junwei Yang Xuejun Zhou
author_sort	Zhiqun Li
title	MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
title_short	MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
title_full	MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
title_fullStr	MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
title_full_unstemmed	MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)
title_sort	microrna hsa-mir-150-5p inhibits nasopharyngeal carcinogenesis by suppressing pycr1 (pyrroline-5-carboxylate reductase 1)
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/5df6ed47594d43afa7092045f621e1df
work_keys_str_mv	AT zhiqunli micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1 AT xiaoliuzhou micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1 AT jiajunhuang micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1 AT zhencaixu micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1 AT chengliangxing micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1 AT junweiyang micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1 AT xuejunzhou micrornahsamir1505pinhibitsnasopharyngealcarcinogenesisbysuppressingpycr1pyrroline5carboxylatereductase1
_version_	1718444717053575168

MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)

Ejemplares similares