Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model
Context and Aim: Lipid overnutrition in female rabbits, from prepuberty, leads to impaired metabolism (dyslipidemia and increased adiposity) and follicular atresia, and, when continued during gestation, affects offspring phenotype with intrauterine growth retardation (IUGR) and leads to placental an...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/5dfb565b9ef24909b50da4d8fcc521ac |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:5dfb565b9ef24909b50da4d8fcc521ac |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:5dfb565b9ef24909b50da4d8fcc521ac2021-12-01T01:50:15ZImportance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model1664-042X10.3389/fphys.2021.784268https://doaj.org/article/5dfb565b9ef24909b50da4d8fcc521ac2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.784268/fullhttps://doaj.org/toc/1664-042XContext and Aim: Lipid overnutrition in female rabbits, from prepuberty, leads to impaired metabolism (dyslipidemia and increased adiposity) and follicular atresia, and, when continued during gestation, affects offspring phenotype with intrauterine growth retardation (IUGR) and leads to placental and lipid metabolism abnormalities. Growth retardation is already observed in embryo stage, indicating a possible implication of periconceptional exposure. The objective of this study was to discriminate the effects of preconception and gestational exposures on feto-placental development.Materials and Methods: Rabbit 1-day zygotes were collected from female donors under control (CD) or high-fat-high-cholesterol (HD) diet and surgically transferred to the left and right uterus, respectively, of each H (n = 6) or C (n = 7) synchronized recipients. Close to term, four combinations, CC (n = 10), CH (n = 13), HC (n = 13), and HH (n = 6), of feto-placental units were collected, for biometry analyses. Fatty acid (FA) profiles were determined in placental labyrinth, decidua, fetal plasma, and fetal liver by gas chromatography and explored further by principal component analysis (PCA). Candidate gene expression was also analyzed by RT-qPCR in the placenta and fetal liver. Data were analyzed by Kruskal–Wallis followed by Dunn’s pairwise comparison test. Combinations of different data sets were combined and explored by multifactorial analysis (MFA).Results: Compared to controls, HH fetuses were hypotrophic with reduced placental efficiency and altered organogenesis, CH presented heavier placenta but less efficient, whereas HC presented a normal biometry. However, the MFA resulted in a good separation of the four groups, discriminating the effects of each period of exposure. HD during gestation led to reduced gene expression (nutrient transport and metabolism) and big changes in FA profiles in both tissues with increased membrane linoleic acid, lipid storage, and polyunsaturated-to-saturated FA ratios. Pre-conception exposure had a major effect on fetal biometry and organogenesis in HH, with specific changes in FA profiles (increased MUFAs and decreased LCPUFAs).Conclusion: Embryo origin left traces in end-gestation feto-placental unit; however, maternal diet during gestation played a major role, either negative (HD) or positive (control). Thus, an H embryo developed favorably when transferred to a C recipient (HC) with normal biometry at term, despite disturbed and altered FA profiles.Delphine Rousseau-RalliardDelphine Rousseau-RalliardMarie-Christine AubrièreMarie-Christine AubrièreNathalie DanielNathalie DanielMichèle DahirelMichèle DahirelGwendoline MorinAudrey PrézelinAudrey PrézelinJérémy BertrandCatherine ReyPascale Chavatte-PalmerPascale Chavatte-PalmerAnne Couturier-TarradeAnne Couturier-TarradeFrontiers Media S.A.articlefetal programmingplacentaliverfatty acidsgene expressionrabbitPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
fetal programming placenta liver fatty acids gene expression rabbit Physiology QP1-981 |
| spellingShingle |
fetal programming placenta liver fatty acids gene expression rabbit Physiology QP1-981 Delphine Rousseau-Ralliard Delphine Rousseau-Ralliard Marie-Christine Aubrière Marie-Christine Aubrière Nathalie Daniel Nathalie Daniel Michèle Dahirel Michèle Dahirel Gwendoline Morin Audrey Prézelin Audrey Prézelin Jérémy Bertrand Catherine Rey Pascale Chavatte-Palmer Pascale Chavatte-Palmer Anne Couturier-Tarrade Anne Couturier-Tarrade Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model |
| description |
Context and Aim: Lipid overnutrition in female rabbits, from prepuberty, leads to impaired metabolism (dyslipidemia and increased adiposity) and follicular atresia, and, when continued during gestation, affects offspring phenotype with intrauterine growth retardation (IUGR) and leads to placental and lipid metabolism abnormalities. Growth retardation is already observed in embryo stage, indicating a possible implication of periconceptional exposure. The objective of this study was to discriminate the effects of preconception and gestational exposures on feto-placental development.Materials and Methods: Rabbit 1-day zygotes were collected from female donors under control (CD) or high-fat-high-cholesterol (HD) diet and surgically transferred to the left and right uterus, respectively, of each H (n = 6) or C (n = 7) synchronized recipients. Close to term, four combinations, CC (n = 10), CH (n = 13), HC (n = 13), and HH (n = 6), of feto-placental units were collected, for biometry analyses. Fatty acid (FA) profiles were determined in placental labyrinth, decidua, fetal plasma, and fetal liver by gas chromatography and explored further by principal component analysis (PCA). Candidate gene expression was also analyzed by RT-qPCR in the placenta and fetal liver. Data were analyzed by Kruskal–Wallis followed by Dunn’s pairwise comparison test. Combinations of different data sets were combined and explored by multifactorial analysis (MFA).Results: Compared to controls, HH fetuses were hypotrophic with reduced placental efficiency and altered organogenesis, CH presented heavier placenta but less efficient, whereas HC presented a normal biometry. However, the MFA resulted in a good separation of the four groups, discriminating the effects of each period of exposure. HD during gestation led to reduced gene expression (nutrient transport and metabolism) and big changes in FA profiles in both tissues with increased membrane linoleic acid, lipid storage, and polyunsaturated-to-saturated FA ratios. Pre-conception exposure had a major effect on fetal biometry and organogenesis in HH, with specific changes in FA profiles (increased MUFAs and decreased LCPUFAs).Conclusion: Embryo origin left traces in end-gestation feto-placental unit; however, maternal diet during gestation played a major role, either negative (HD) or positive (control). Thus, an H embryo developed favorably when transferred to a C recipient (HC) with normal biometry at term, despite disturbed and altered FA profiles. |
| format |
article |
| author |
Delphine Rousseau-Ralliard Delphine Rousseau-Ralliard Marie-Christine Aubrière Marie-Christine Aubrière Nathalie Daniel Nathalie Daniel Michèle Dahirel Michèle Dahirel Gwendoline Morin Audrey Prézelin Audrey Prézelin Jérémy Bertrand Catherine Rey Pascale Chavatte-Palmer Pascale Chavatte-Palmer Anne Couturier-Tarrade Anne Couturier-Tarrade |
| author_facet |
Delphine Rousseau-Ralliard Delphine Rousseau-Ralliard Marie-Christine Aubrière Marie-Christine Aubrière Nathalie Daniel Nathalie Daniel Michèle Dahirel Michèle Dahirel Gwendoline Morin Audrey Prézelin Audrey Prézelin Jérémy Bertrand Catherine Rey Pascale Chavatte-Palmer Pascale Chavatte-Palmer Anne Couturier-Tarrade Anne Couturier-Tarrade |
| author_sort |
Delphine Rousseau-Ralliard |
| title |
Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model |
| title_short |
Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model |
| title_full |
Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model |
| title_fullStr |
Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model |
| title_full_unstemmed |
Importance of Windows of Exposure to Maternal High-Fat Diet and Feto-Placental Effects: Discrimination Between Pre-conception and Gestational Periods in a Rabbit Model |
| title_sort |
importance of windows of exposure to maternal high-fat diet and feto-placental effects: discrimination between pre-conception and gestational periods in a rabbit model |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/5dfb565b9ef24909b50da4d8fcc521ac |
| work_keys_str_mv |
AT delphinerousseauralliard importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT delphinerousseauralliard importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT mariechristineaubriere importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT mariechristineaubriere importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT nathaliedaniel importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT nathaliedaniel importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT micheledahirel importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT micheledahirel importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT gwendolinemorin importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT audreyprezelin importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT audreyprezelin importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT jeremybertrand importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT catherinerey importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT pascalechavattepalmer importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT pascalechavattepalmer importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT annecouturiertarrade importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel AT annecouturiertarrade importanceofwindowsofexposuretomaternalhighfatdietandfetoplacentaleffectsdiscriminationbetweenpreconceptionandgestationalperiodsinarabbitmodel |
| _version_ |
1718405961518940160 |