Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium

Abstract Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells...

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Autores principales:	Heidi Hongisto, Antti Jylhä, Janika Nättinen, Jochen Rieck, Tanja Ilmarinen, Zoltán Veréb, Ulla Aapola, Roger Beuerman, Goran Petrovski, Hannu Uusitalo, Heli Skottman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5dfb8d40194e4fada1a0660c2d028f4e
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spelling	oai:doaj.org-article:5dfb8d40194e4fada1a0660c2d028f4e2021-12-02T12:32:00ZComparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium10.1038/s41598-017-06233-92045-2322https://doaj.org/article/5dfb8d40194e4fada1a0660c2d028f4e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06233-9https://doaj.org/toc/2045-2322Abstract Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells have been thoroughly characterized at the gene level but their protein expression profile has not been studied at larger scale. In this study, proteomic analysis was used to compare hESC-RPE cells differentiated from two independent hESC lines, to primary human RPE (hRPE) using Isobaric tags for relative quantitation (iTRAQ). 1041 common proteins were present in both hESC-RPE cells and native hRPE with majority of the proteins similarly regulated. The hESC-RPE proteome reflected that of normal hRPE with a large number of metabolic, mitochondrial, cytoskeletal, and transport proteins expressed. No signs of increased stress, apoptosis, immune response, proliferation, or retinal degeneration related changes were noted in hESC-RPE, while important RPE specific proteins involved in key RPE functions such as visual cycle and phagocytosis, could be detected in the hESC-RPE. Overall, the results indicated that the proteome of the hESC-RPE cells closely resembled that of their native counterparts.Heidi HongistoAntti JylhäJanika NättinenJochen RieckTanja IlmarinenZoltán VerébUlla AapolaRoger BeuermanGoran PetrovskiHannu UusitaloHeli SkottmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heidi Hongisto Antti Jylhä Janika Nättinen Jochen Rieck Tanja Ilmarinen Zoltán Veréb Ulla Aapola Roger Beuerman Goran Petrovski Hannu Uusitalo Heli Skottman Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
description	Abstract Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells have been thoroughly characterized at the gene level but their protein expression profile has not been studied at larger scale. In this study, proteomic analysis was used to compare hESC-RPE cells differentiated from two independent hESC lines, to primary human RPE (hRPE) using Isobaric tags for relative quantitation (iTRAQ). 1041 common proteins were present in both hESC-RPE cells and native hRPE with majority of the proteins similarly regulated. The hESC-RPE proteome reflected that of normal hRPE with a large number of metabolic, mitochondrial, cytoskeletal, and transport proteins expressed. No signs of increased stress, apoptosis, immune response, proliferation, or retinal degeneration related changes were noted in hESC-RPE, while important RPE specific proteins involved in key RPE functions such as visual cycle and phagocytosis, could be detected in the hESC-RPE. Overall, the results indicated that the proteome of the hESC-RPE cells closely resembled that of their native counterparts.
format	article
author	Heidi Hongisto Antti Jylhä Janika Nättinen Jochen Rieck Tanja Ilmarinen Zoltán Veréb Ulla Aapola Roger Beuerman Goran Petrovski Hannu Uusitalo Heli Skottman
author_facet	Heidi Hongisto Antti Jylhä Janika Nättinen Jochen Rieck Tanja Ilmarinen Zoltán Veréb Ulla Aapola Roger Beuerman Goran Petrovski Hannu Uusitalo Heli Skottman
author_sort	Heidi Hongisto
title	Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
title_short	Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
title_full	Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
title_fullStr	Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
title_full_unstemmed	Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
title_sort	comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5dfb8d40194e4fada1a0660c2d028f4e
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Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium

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