Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study

Neuroinflammatory mechanisms with glial cell activation have been implicated in the pathogenic process of Alzheimer’s disease (AD). Activation of the NLRP3 inflammasome is an essential component of the neuroinflammatory response. A role for NLRP3 activation in AD is supported by both in vitro and in...

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Autores principales:	Hao Tang, Michael Harte
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	NLRP3 inflammasome neuroinflammation Alzheimer’s disease gene expression post-mortem Genetics QH426-470
Acceso en línea:	https://doaj.org/article/5e00143ae72843f9811f3164dd297d4f
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spelling	oai:doaj.org-article:5e00143ae72843f9811f3164dd297d4f2021-11-25T17:41:32ZInvestigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study10.3390/genes121117532073-4425https://doaj.org/article/5e00143ae72843f9811f3164dd297d4f2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1753https://doaj.org/toc/2073-4425Neuroinflammatory mechanisms with glial cell activation have been implicated in the pathogenic process of Alzheimer’s disease (AD). Activation of the NLRP3 inflammasome is an essential component of the neuroinflammatory response. A role for NLRP3 activation in AD is supported by both in vitro and in vivo preclinical studies with little direct investigation of AD brain tissue. RNA expression of genes of three glial cell markers, HLA-DRA, AIF-1 and GFAP; the components of the NLRP3 inflammasome NLRP3, ASC, and caspase-1; and downstream pre-inflammatory cytokines IL-1 β and IL-18, were investigated in the temporal cortex of AD patients and age- and sex-matched controls. Protein expression of GFAP was also assessed. Increases in both mRNA and protein expression were observed for GFAP in AD. There were no significant changes in other NLRP3 activation markers between groups. Our results indicate the involvement of astrocyte activation in AD, particularly in more severe patients. We found no evidence for the specific involvement of the NLRP3 inflammasome.Hao TangMichael HarteMDPI AGarticleNLRP3 inflammasomeneuroinflammationAlzheimer’s diseasegene expressionpost-mortemGeneticsQH426-470ENGenes, Vol 12, Iss 1753, p 1753 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NLRP3 inflammasome neuroinflammation Alzheimer’s disease gene expression post-mortem Genetics QH426-470
spellingShingle	NLRP3 inflammasome neuroinflammation Alzheimer’s disease gene expression post-mortem Genetics QH426-470 Hao Tang Michael Harte Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study
description	Neuroinflammatory mechanisms with glial cell activation have been implicated in the pathogenic process of Alzheimer’s disease (AD). Activation of the NLRP3 inflammasome is an essential component of the neuroinflammatory response. A role for NLRP3 activation in AD is supported by both in vitro and in vivo preclinical studies with little direct investigation of AD brain tissue. RNA expression of genes of three glial cell markers, HLA-DRA, AIF-1 and GFAP; the components of the NLRP3 inflammasome NLRP3, ASC, and caspase-1; and downstream pre-inflammatory cytokines IL-1 β and IL-18, were investigated in the temporal cortex of AD patients and age- and sex-matched controls. Protein expression of GFAP was also assessed. Increases in both mRNA and protein expression were observed for GFAP in AD. There were no significant changes in other NLRP3 activation markers between groups. Our results indicate the involvement of astrocyte activation in AD, particularly in more severe patients. We found no evidence for the specific involvement of the NLRP3 inflammasome.
format	article
author	Hao Tang Michael Harte
author_facet	Hao Tang Michael Harte
author_sort	Hao Tang
title	Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study
title_short	Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study
title_full	Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study
title_fullStr	Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study
title_full_unstemmed	Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study
title_sort	investigating markers of the nlrp3 inflammasome pathway in alzheimer’s disease: a human post-mortem study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5e00143ae72843f9811f3164dd297d4f
work_keys_str_mv	AT haotang investigatingmarkersofthenlrp3inflammasomepathwayinalzheimersdiseaseahumanpostmortemstudy AT michaelharte investigatingmarkersofthenlrp3inflammasomepathwayinalzheimersdiseaseahumanpostmortemstudy
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Investigating Markers of the NLRP3 Inflammasome Pathway in Alzheimer’s Disease: A Human Post-Mortem Study

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