Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen

Two fixation methods can affect the quality of cytological staining derived from fine-needle aspiration biopsy, but both fixation methods have drawbacks. Dry fixation can cause cell rupture, while wet fixation requires a longer time and costs more, so dry fixation is more often used. The purpose of...

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Autores principales:	Fitri Nuroini, Zaldy Alfanda Hidayat, Tulus Ariyadi
Formato:	article
Lenguaje:	ID
Publicado:	Poltekkes Kemenkes Yogyakarta 2021
Materias:	air-dried fixation wet fixation fnab diff-quick Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5e0ae9d6f5ae4d56bea09d2ae718ce3c
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spelling	oai:doaj.org-article:5e0ae9d6f5ae4d56bea09d2ae718ce3c2021-11-13T13:40:05ZAir-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen2338-56342580-019110.29238/teknolabjournal.v10i1.279https://doaj.org/article/5e0ae9d6f5ae4d56bea09d2ae718ce3c2021-08-01T00:00:00Zhttps://teknolabjournal.com/index.php/Jtl/article/view/279https://doaj.org/toc/2338-5634https://doaj.org/toc/2580-0191Two fixation methods can affect the quality of cytological staining derived from fine-needle aspiration biopsy, but both fixation methods have drawbacks. Dry fixation can cause cell rupture, while wet fixation requires a longer time and costs more, so dry fixation is more often used. The purpose of this study was to compare the results of the Diff-Quick staining of cytological preparations that were fixed with the dry fixation method and the wet fixation method on FNAB samples. This study was an experimental study with 36 samples of cytology preparations from FNAB, divided into three groups, the control group, dry fixation group, and wet fixation group. Staining dry fixation preparations gave four poor results, five good preparations, and three very good preparations while staining wet fixation preparations gave 0 poor results, eight good preparations, and four very good preparations. This shows that the staining results of wet-fixed preparations tend to be of better quality and more consistent than the staining results of dry-fixed preparations. Even though statistically through the test Post-Hoc showed no difference, the quality of Diff-Quick staining of cytological preparations fixed by wet fixation method was better than dry fixation, so the wet fixation method was more recommended for cytological preparations than FNAB.Fitri NuroiniZaldy Alfanda HidayatTulus AriyadiPoltekkes Kemenkes Yogyakartaarticleair-dried fixationwet fixationfnabdiff-quickMedicine (General)R5-920IDJurnal Teknologi Laboratorium, Vol 10, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	ID
topic	air-dried fixation wet fixation fnab diff-quick Medicine (General) R5-920
spellingShingle	air-dried fixation wet fixation fnab diff-quick Medicine (General) R5-920 Fitri Nuroini Zaldy Alfanda Hidayat Tulus Ariyadi Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen
description	Two fixation methods can affect the quality of cytological staining derived from fine-needle aspiration biopsy, but both fixation methods have drawbacks. Dry fixation can cause cell rupture, while wet fixation requires a longer time and costs more, so dry fixation is more often used. The purpose of this study was to compare the results of the Diff-Quick staining of cytological preparations that were fixed with the dry fixation method and the wet fixation method on FNAB samples. This study was an experimental study with 36 samples of cytology preparations from FNAB, divided into three groups, the control group, dry fixation group, and wet fixation group. Staining dry fixation preparations gave four poor results, five good preparations, and three very good preparations while staining wet fixation preparations gave 0 poor results, eight good preparations, and four very good preparations. This shows that the staining results of wet-fixed preparations tend to be of better quality and more consistent than the staining results of dry-fixed preparations. Even though statistically through the test Post-Hoc showed no difference, the quality of Diff-Quick staining of cytological preparations fixed by wet fixation method was better than dry fixation, so the wet fixation method was more recommended for cytological preparations than FNAB.
format	article
author	Fitri Nuroini Zaldy Alfanda Hidayat Tulus Ariyadi
author_facet	Fitri Nuroini Zaldy Alfanda Hidayat Tulus Ariyadi
author_sort	Fitri Nuroini
title	Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen
title_short	Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen
title_full	Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen
title_fullStr	Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen
title_full_unstemmed	Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen
title_sort	air-dried and wet fixation on fine needle aspiration biopsy (fnab) specimen
publisher	Poltekkes Kemenkes Yogyakarta
publishDate	2021
url	https://doaj.org/article/5e0ae9d6f5ae4d56bea09d2ae718ce3c
work_keys_str_mv	AT fitrinuroini airdriedandwetfixationonfineneedleaspirationbiopsyfnabspecimen AT zaldyalfandahidayat airdriedandwetfixationonfineneedleaspirationbiopsyfnabspecimen AT tulusariyadi airdriedandwetfixationonfineneedleaspirationbiopsyfnabspecimen
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Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen

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