α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.

Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we s...

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Autores principales:	Gisele Macêdo Rodrigues da Cunha, Maíra Araújo Azevedo, Denise Silva Nogueira, Marianna de Carvalho Clímaco, Edward Valencia Ayala, Juan Atilio Jimenez Chunga, Raul Jesus Ynocente La Valle, Lucia Maria da Cunha Galvão, Egler Chiari, Carlos Ramon Nascimento Brito, Rodrigo Pedro Soares, Paula Monalisa Nogueira, Ricardo Toshio Fujiwara, Ricardo Gazzinelli, Robert Hincapie, Carlos-Sanhueza Chaves, Fabricio Marcus Silva Oliveira, M G Finn, Alexandre Ferreira Marques
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28
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spelling	oai:doaj.org-article:5e151268acdb46dfbf28a814c81a6e282021-11-25T06:33:27Zα-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.1935-27271935-273510.1371/journal.pntd.0009613https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e282021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009613https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we show that immunization with a virus-like particle vaccine displaying a high density of the immunogenic α-Gal trisaccharide (Qβ-αGal) induced several beneficial effects concerning acute and chronic T. cruzi infection in α1,3-galactosyltransferase knockout mice. Approximately 60% of these animals were protected from initial infection with high parasite loads. Vaccinated animals also produced high anti-αGal IgG antibody titers, improved IFN-γ and IL-12 cytokine production, and controlled parasitemia in the acute phase at 8 days post-infection (dpi) for the Y strain and 22 dpi for the Colombian strain. In the chronic stage of infection (36 and 190 dpi, respectively), all of the vaccinated group survived, showing significantly decreased heart inflammation and clearance of amastigote nests from the heart tissue.Gisele Macêdo Rodrigues da CunhaMaíra Araújo AzevedoDenise Silva NogueiraMarianna de Carvalho ClímacoEdward Valencia AyalaJuan Atilio Jimenez ChungaRaul Jesus Ynocente La ValleLucia Maria da Cunha GalvãoEgler ChiariCarlos Ramon Nascimento BritoRodrigo Pedro SoaresPaula Monalisa NogueiraRicardo Toshio FujiwaraRicardo GazzinelliRobert HincapieCarlos-Sanhueza ChavesFabricio Marcus Silva OliveiraM G FinnAlexandre Ferreira MarquesPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009613 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
description	Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we show that immunization with a virus-like particle vaccine displaying a high density of the immunogenic α-Gal trisaccharide (Qβ-αGal) induced several beneficial effects concerning acute and chronic T. cruzi infection in α1,3-galactosyltransferase knockout mice. Approximately 60% of these animals were protected from initial infection with high parasite loads. Vaccinated animals also produced high anti-αGal IgG antibody titers, improved IFN-γ and IL-12 cytokine production, and controlled parasitemia in the acute phase at 8 days post-infection (dpi) for the Y strain and 22 dpi for the Colombian strain. In the chronic stage of infection (36 and 190 dpi, respectively), all of the vaccinated group survived, showing significantly decreased heart inflammation and clearance of amastigote nests from the heart tissue.
format	article
author	Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques
author_facet	Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques
author_sort	Gisele Macêdo Rodrigues da Cunha
title	α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
title_short	α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
title_full	α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
title_fullStr	α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
title_full_unstemmed	α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
title_sort	α-gal immunization positively impacts trypanosoma cruzi colonization of heart tissue in a mouse model.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28
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α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.

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