Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach

Abstract Background Mosquitoes transmit a variety of diseases. Due to widespread insecticide resistance, new effective pesticides are urgently needed. Entomopathogenic fungi are widely utilized to control pest insects in agriculture. We hypothesized that certain fungal metabolites may be effective i...

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Autores principales:	Liang Jin, Guodong Niu, Limei Guan, Julian Ramelow, Zhigao Zhan, Xi Zhou, Jun Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Mosquito Vector-borne diseases Pesticides Penicillium toxicarium Anopheles gambiae Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/5e19a464f52340cb87668c10f15f95e4
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spelling	oai:doaj.org-article:5e19a464f52340cb87668c10f15f95e42021-12-05T12:17:05ZDiscovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach10.1186/s13071-021-05089-31756-3305https://doaj.org/article/5e19a464f52340cb87668c10f15f95e42021-12-01T00:00:00Zhttps://doi.org/10.1186/s13071-021-05089-3https://doaj.org/toc/1756-3305Abstract Background Mosquitoes transmit a variety of diseases. Due to widespread insecticide resistance, new effective pesticides are urgently needed. Entomopathogenic fungi are widely utilized to control pest insects in agriculture. We hypothesized that certain fungal metabolites may be effective insecticides against mosquitoes. Methods A high-throughput cytotoxicity-based screening approach was developed to search for insecticidal compounds in our newly established global fungal extract library. We first determined cell survival rates after adding various fungal extracts. Candidate insecticides were further analyzed using traditional larval and adult survival bioassays. Results Twelve ethyl acetate extracts from a total of 192 fungal extracts displayed > 85% inhibition of cabbage looper ovary cell proliferation. Ten of these 12 candidates were confirmed to be toxic to Anopheles gambiae Sua5B cell line, and six showed > 85% inhibition of Anopheles mosquito cell growth. Further bioassays determined a LC50, the lethal concentration that kills 50% of larval or adult mosquitoes, of 122 µg/mL and 1.7 µg/mosquito, respectively, after 24 h for extract 76F6 from Penicillium toxicarium. Conclusions We established a high-throughput MTT-based cytotoxicity screening approach for the discovery of new mosquitocides from fungal extracts. We discovered a candidate extract from P. toxicarium that exhibited high toxicity to mosquito larvae and adults, and thus were able to demonstrate the value of our recently developed approach. The active fungal extracts discovered here are ideal candidates for further development as mosquitocides. Graphical abstractLiang JinGuodong NiuLimei GuanJulian RamelowZhigao ZhanXi ZhouJun LiBMCarticleMosquitoVector-borne diseasesPesticidesPenicillium toxicariumAnopheles gambiaeInfectious and parasitic diseasesRC109-216ENParasites & Vectors, Vol 14, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mosquito Vector-borne diseases Pesticides Penicillium toxicarium Anopheles gambiae Infectious and parasitic diseases RC109-216
spellingShingle	Mosquito Vector-borne diseases Pesticides Penicillium toxicarium Anopheles gambiae Infectious and parasitic diseases RC109-216 Liang Jin Guodong Niu Limei Guan Julian Ramelow Zhigao Zhan Xi Zhou Jun Li Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
description	Abstract Background Mosquitoes transmit a variety of diseases. Due to widespread insecticide resistance, new effective pesticides are urgently needed. Entomopathogenic fungi are widely utilized to control pest insects in agriculture. We hypothesized that certain fungal metabolites may be effective insecticides against mosquitoes. Methods A high-throughput cytotoxicity-based screening approach was developed to search for insecticidal compounds in our newly established global fungal extract library. We first determined cell survival rates after adding various fungal extracts. Candidate insecticides were further analyzed using traditional larval and adult survival bioassays. Results Twelve ethyl acetate extracts from a total of 192 fungal extracts displayed > 85% inhibition of cabbage looper ovary cell proliferation. Ten of these 12 candidates were confirmed to be toxic to Anopheles gambiae Sua5B cell line, and six showed > 85% inhibition of Anopheles mosquito cell growth. Further bioassays determined a LC50, the lethal concentration that kills 50% of larval or adult mosquitoes, of 122 µg/mL and 1.7 µg/mosquito, respectively, after 24 h for extract 76F6 from Penicillium toxicarium. Conclusions We established a high-throughput MTT-based cytotoxicity screening approach for the discovery of new mosquitocides from fungal extracts. We discovered a candidate extract from P. toxicarium that exhibited high toxicity to mosquito larvae and adults, and thus were able to demonstrate the value of our recently developed approach. The active fungal extracts discovered here are ideal candidates for further development as mosquitocides. Graphical abstract
format	article
author	Liang Jin Guodong Niu Limei Guan Julian Ramelow Zhigao Zhan Xi Zhou Jun Li
author_facet	Liang Jin Guodong Niu Limei Guan Julian Ramelow Zhigao Zhan Xi Zhou Jun Li
author_sort	Liang Jin
title	Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
title_short	Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
title_full	Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
title_fullStr	Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
title_full_unstemmed	Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
title_sort	discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach
publisher	BMC
publishDate	2021
url	https://doaj.org/article/5e19a464f52340cb87668c10f15f95e4
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Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach

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