Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study

Abstract Ulcerative colitis (UC) is a chronic immune-mediated disorder, whose etiology is not fully understood and for which no effective treatment is available. Recently, research has focused on the dysbiosis of gut microbiome in UC. However, the results so far remain inconsistent and insufficient...

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Autores principales:	Oliwia Zakerska-Banaszak, Hanna Tomczak, Marcin Gabryel, Alina Baturo, Lukasz Wolko, Michal Michalak, Natalia Malinska, Dorota Mankowska-Wierzbicka, Piotr Eder, Agnieszka Dobrowolska, Ryszard Slomski, Marzena Skrzypczak-Zielinska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e1b12442e5a494c9a7a4291e8e77c71
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spelling	oai:doaj.org-article:5e1b12442e5a494c9a7a4291e8e77c712021-12-02T13:57:49ZDysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study10.1038/s41598-021-81628-32045-2322https://doaj.org/article/5e1b12442e5a494c9a7a4291e8e77c712021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81628-3https://doaj.org/toc/2045-2322Abstract Ulcerative colitis (UC) is a chronic immune-mediated disorder, whose etiology is not fully understood and for which no effective treatment is available. Recently, research has focused on the dysbiosis of gut microbiome in UC. However, the results so far remain inconsistent and insufficient to understand the microbial component in UC pathogenesis. In this study, we determine specific changes in the gut microbial profile in Polish UC patients compared to healthy subjects for the first time. Using 16S rRNA gene-based analysis we have described the intestinal microbial community in a group of 20 individuals (10 UC patients and 10 controls). Our results after multiple hypothesis testing correction demonstrated substantially lower gut microbiome diversity in UC cases compared to the controls and considerable differences at the phylum level, as well as among 13 bacterial families and 20 bacterial genera (p < 0.05). UC samples were more abundant in Proteobacteria (8.42%), Actinobacteria (6.89%) and Candidate Division TM7 (2.88%) than those of healthy volunteers (2.57%, 2.29% and 0.012%, respectively). On the other hand, Bacteroidetes and Verrucomicrobia were presented at a lower level in UC relative to the controls (14% and 0% vs 27.97% and 4.47%, respectively). In conclusion, our results show a reduced gut microbial diversity in Polish UC patients, a reduction of taxa with an anti-inflammatory impact and an increased abundance of potentially pathogenic bacteria.Oliwia Zakerska-BanaszakHanna TomczakMarcin GabryelAlina BaturoLukasz WolkoMichal MichalakNatalia MalinskaDorota Mankowska-WierzbickaPiotr EderAgnieszka DobrowolskaRyszard SlomskiMarzena Skrzypczak-ZielinskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oliwia Zakerska-Banaszak Hanna Tomczak Marcin Gabryel Alina Baturo Lukasz Wolko Michal Michalak Natalia Malinska Dorota Mankowska-Wierzbicka Piotr Eder Agnieszka Dobrowolska Ryszard Slomski Marzena Skrzypczak-Zielinska Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study
description	Abstract Ulcerative colitis (UC) is a chronic immune-mediated disorder, whose etiology is not fully understood and for which no effective treatment is available. Recently, research has focused on the dysbiosis of gut microbiome in UC. However, the results so far remain inconsistent and insufficient to understand the microbial component in UC pathogenesis. In this study, we determine specific changes in the gut microbial profile in Polish UC patients compared to healthy subjects for the first time. Using 16S rRNA gene-based analysis we have described the intestinal microbial community in a group of 20 individuals (10 UC patients and 10 controls). Our results after multiple hypothesis testing correction demonstrated substantially lower gut microbiome diversity in UC cases compared to the controls and considerable differences at the phylum level, as well as among 13 bacterial families and 20 bacterial genera (p < 0.05). UC samples were more abundant in Proteobacteria (8.42%), Actinobacteria (6.89%) and Candidate Division TM7 (2.88%) than those of healthy volunteers (2.57%, 2.29% and 0.012%, respectively). On the other hand, Bacteroidetes and Verrucomicrobia were presented at a lower level in UC relative to the controls (14% and 0% vs 27.97% and 4.47%, respectively). In conclusion, our results show a reduced gut microbial diversity in Polish UC patients, a reduction of taxa with an anti-inflammatory impact and an increased abundance of potentially pathogenic bacteria.
format	article
author	Oliwia Zakerska-Banaszak Hanna Tomczak Marcin Gabryel Alina Baturo Lukasz Wolko Michal Michalak Natalia Malinska Dorota Mankowska-Wierzbicka Piotr Eder Agnieszka Dobrowolska Ryszard Slomski Marzena Skrzypczak-Zielinska
author_facet	Oliwia Zakerska-Banaszak Hanna Tomczak Marcin Gabryel Alina Baturo Lukasz Wolko Michal Michalak Natalia Malinska Dorota Mankowska-Wierzbicka Piotr Eder Agnieszka Dobrowolska Ryszard Slomski Marzena Skrzypczak-Zielinska
author_sort	Oliwia Zakerska-Banaszak
title	Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study
title_short	Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study
title_full	Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study
title_fullStr	Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study
title_full_unstemmed	Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study
title_sort	dysbiosis of gut microbiota in polish patients with ulcerative colitis: a pilot study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5e1b12442e5a494c9a7a4291e8e77c71
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Dysbiosis of gut microbiota in Polish patients with ulcerative colitis: a pilot study

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