Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura

Abstract While it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. Insect chemosensory proteins (CSPs) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. Recent st...

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Autores principales:	Xin Yi, Jiangwei Qi, Xiaofan Zhou, Mei Ying Hu, Guo Hua Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e20672cff094b62a23b2065bb31a9a7
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spelling	oai:doaj.org-article:5e20672cff094b62a23b2065bb31a9a72021-12-02T16:06:04ZDifferential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura10.1038/s41598-017-00403-52045-2322https://doaj.org/article/5e20672cff094b62a23b2065bb31a9a72017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00403-5https://doaj.org/toc/2045-2322Abstract While it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. Insect chemosensory proteins (CSPs) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. Recent studies have identified a number of CSPs expressed in midgut in Lepidoptera insects, which started to reveal their roles in chemical recognition and stimulating appetite in midgut. In this study, we examined expression patterns in midgut of 21 Spodoptera litura CSPs (SlitCSPs) characterized from a previously reported transcriptome, and three CSPs were identified to be expressed highly in midgut. The orthologous relationships between midgut expressed CSPs in S. litura and those in Bombyx mori and Plutella xylostella also suggest a conserved pattern of CSP expression in midgut. We further demonstrated that the expression of midgut-CSPs may change in response to different host plants, and SlitCSPs could bind typical chemicals from host plant in vitro. Overall, our results suggested midgut expressed SlitCSPs may have functional roles, likely contributing to specialization and adaption to different ecosystems. Better knowledge of this critical component of the chemsensation signaling pathways in midguts may improve our understanding of food preference processes in a new perspective.Xin YiJiangwei QiXiaofan ZhouMei Ying HuGuo Hua ZhongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Yi Jiangwei Qi Xiaofan Zhou Mei Ying Hu Guo Hua Zhong Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
description	Abstract While it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. Insect chemosensory proteins (CSPs) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. Recent studies have identified a number of CSPs expressed in midgut in Lepidoptera insects, which started to reveal their roles in chemical recognition and stimulating appetite in midgut. In this study, we examined expression patterns in midgut of 21 Spodoptera litura CSPs (SlitCSPs) characterized from a previously reported transcriptome, and three CSPs were identified to be expressed highly in midgut. The orthologous relationships between midgut expressed CSPs in S. litura and those in Bombyx mori and Plutella xylostella also suggest a conserved pattern of CSP expression in midgut. We further demonstrated that the expression of midgut-CSPs may change in response to different host plants, and SlitCSPs could bind typical chemicals from host plant in vitro. Overall, our results suggested midgut expressed SlitCSPs may have functional roles, likely contributing to specialization and adaption to different ecosystems. Better knowledge of this critical component of the chemsensation signaling pathways in midguts may improve our understanding of food preference processes in a new perspective.
format	article
author	Xin Yi Jiangwei Qi Xiaofan Zhou Mei Ying Hu Guo Hua Zhong
author_facet	Xin Yi Jiangwei Qi Xiaofan Zhou Mei Ying Hu Guo Hua Zhong
author_sort	Xin Yi
title	Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
title_short	Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
title_full	Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
title_fullStr	Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
title_full_unstemmed	Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
title_sort	differential expression of chemosensory-protein genes in midguts in response to diet of spodoptera litura
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5e20672cff094b62a23b2065bb31a9a7
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Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura

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