Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS

Abstract Lipoteichoic acid (LTA) of Gram-positive bacteria is regarded as the counterpart biomolecule of lipopolysaccharide (LPS) of Gram-negative bacteria because of their structural and immunological similarities. Although LPS induces a strong polyclonal expansion of B cells, little is known about...

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Autores principales:	Seok-Seong Kang, Sun Kyung Kim, Jung Eun Baik, Eun Byeol Ko, Ki Bum Ahn, Cheol-Heui Yun, Seung Hyun Han
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e2099abb04b4bba83135d22b595c08e
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spelling	oai:doaj.org-article:5e2099abb04b4bba83135d22b595c08e2021-12-02T15:09:01ZStaphylococcal LTA antagonizes the B cell-mitogenic potential of LPS10.1038/s41598-018-19653-y2045-2322https://doaj.org/article/5e2099abb04b4bba83135d22b595c08e2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19653-yhttps://doaj.org/toc/2045-2322Abstract Lipoteichoic acid (LTA) of Gram-positive bacteria is regarded as the counterpart biomolecule of lipopolysaccharide (LPS) of Gram-negative bacteria because of their structural and immunological similarities. Although LPS induces a strong polyclonal expansion of B cells, little is known about the effect of LTA on B cell proliferation. In the present study, we prepared LTAs from Gram-positive bacteria and examined their effect on splenic B cell proliferation. Unlike LPS, LTA did not induce B cell proliferation. Instead, Staphylococcus aureus LTA (Sa.LTA) appeared to inhibit LPS-induced B cell proliferation in vitro, ex vivo, and in vivo models. Such effect was observed neither in splenocytes from Toll-like receptor 2 (TLR2)-deficient mice nor in the purified splenic B cells. Furthermore, decreased ERK phosphorylation appeared to be responsible for this phenomenon. Collectively, our results support that Sa.LTA inhibited LPS-induced B cell proliferation through the decrease of ERK phosphorylation via TLR2 signaling pathway.Seok-Seong KangSun Kyung KimJung Eun BaikEun Byeol KoKi Bum AhnCheol-Heui YunSeung Hyun HanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seok-Seong Kang Sun Kyung Kim Jung Eun Baik Eun Byeol Ko Ki Bum Ahn Cheol-Heui Yun Seung Hyun Han Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS
description	Abstract Lipoteichoic acid (LTA) of Gram-positive bacteria is regarded as the counterpart biomolecule of lipopolysaccharide (LPS) of Gram-negative bacteria because of their structural and immunological similarities. Although LPS induces a strong polyclonal expansion of B cells, little is known about the effect of LTA on B cell proliferation. In the present study, we prepared LTAs from Gram-positive bacteria and examined their effect on splenic B cell proliferation. Unlike LPS, LTA did not induce B cell proliferation. Instead, Staphylococcus aureus LTA (Sa.LTA) appeared to inhibit LPS-induced B cell proliferation in vitro, ex vivo, and in vivo models. Such effect was observed neither in splenocytes from Toll-like receptor 2 (TLR2)-deficient mice nor in the purified splenic B cells. Furthermore, decreased ERK phosphorylation appeared to be responsible for this phenomenon. Collectively, our results support that Sa.LTA inhibited LPS-induced B cell proliferation through the decrease of ERK phosphorylation via TLR2 signaling pathway.
format	article
author	Seok-Seong Kang Sun Kyung Kim Jung Eun Baik Eun Byeol Ko Ki Bum Ahn Cheol-Heui Yun Seung Hyun Han
author_facet	Seok-Seong Kang Sun Kyung Kim Jung Eun Baik Eun Byeol Ko Ki Bum Ahn Cheol-Heui Yun Seung Hyun Han
author_sort	Seok-Seong Kang
title	Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS
title_short	Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS
title_full	Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS
title_fullStr	Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS
title_full_unstemmed	Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS
title_sort	staphylococcal lta antagonizes the b cell-mitogenic potential of lps
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5e2099abb04b4bba83135d22b595c08e
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_version_	1718387940083630080

Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS

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