Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway

Background: Cyrtopodion scabrum is a kind of lizard, widely distributed in southwestern, central, and eastern Iran. In our previous studies, we showed the selective anticancer properties of Cyrtopodion scabrum extract (CSE) against certain gastrointestinal cancer cell lines with no significant harmf...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mojtaba Rashidi, Atefeh Seghatoleslam
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2021
Materias:	natural anticancer product gecko (gekko) extract cyrtopodion scabrum western blot analysis real-time pcr flow cytometry p53 signaling pathway Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/5e28d9407da249108c2f43225fcc7b38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5e28d9407da249108c2f43225fcc7b38
record_format	dspace
spelling	oai:doaj.org-article:5e28d9407da249108c2f43225fcc7b382021-11-29T10:40:47ZInduction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway2008-67092008-668710.30476/mejc.2021.86645.1363https://doaj.org/article/5e28d9407da249108c2f43225fcc7b382021-10-01T00:00:00Zhttps://mejc.sums.ac.ir/article_47542_0229ea4731c68b7fb9d48d569b7b83f0.pdfhttps://doaj.org/toc/2008-6709https://doaj.org/toc/2008-6687Background: Cyrtopodion scabrum is a kind of lizard, widely distributed in southwestern, central, and eastern Iran. In our previous studies, we showed the selective anticancer properties of Cyrtopodion scabrum extract (CSE) against certain gastrointestinal cancer cell lines with no significant harmful effects on the normal cells. Method: This in vitro study was conducted to investigate the mechanism of action of CSE in SW742 (colon cancer cell line) using western blotting, real-time polymerase chain reaction (PCR), and PI staining/flow cytometry analysis. Results: Western blot analysis showed that CSE upregulates P53 protein expression significantly in SW742 cancer cell line, while QRT-PCR results revealed that P53 mRNA did not increase accordingly. It is proposed that TP53 protein play its role as a tumor suppressor through the protein stability mechanism and not through the increase in the gene expression. Considering this fact, for TP53 stabilization, two of its target genes, p21 and mdm2, were regulated. We evaluated the mRNA expression of these two genes as well. The obtained data showed a significant increase in both genes by CSE (1000 μg/ml), suggesting that the induction of G2 cell cycle arrest, which we previously reported, may happen through P53 and P21over-expression. No significant decrease was observed in apoptosis when SW742 cells were co-treated with CSE and the inhibitor of P53 transcriptional activity, (PFT)-α, indicating that the induction of apoptosis with CSE did not occur through P53-dependent transcriptional activity. Conclusion: The obtained results herein revealed that the observed anticancer effect of CSE may occur through TP53 upregulation, yet with P53-independent transcriptional activity.Mojtaba RashidiAtefeh SeghatoleslamShiraz University of Medical Sciencesarticlenatural anticancer productgecko (gekko) extractcyrtopodion scabrumwestern blot analysisreal-time pcrflow cytometryp53 signaling pathwayNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENMiddle East Journal of Cancer , Vol 12, Iss 4, Pp 466-472 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	natural anticancer product gecko (gekko) extract cyrtopodion scabrum western blot analysis real-time pcr flow cytometry p53 signaling pathway Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	natural anticancer product gecko (gekko) extract cyrtopodion scabrum western blot analysis real-time pcr flow cytometry p53 signaling pathway Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Mojtaba Rashidi Atefeh Seghatoleslam Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway
description	Background: Cyrtopodion scabrum is a kind of lizard, widely distributed in southwestern, central, and eastern Iran. In our previous studies, we showed the selective anticancer properties of Cyrtopodion scabrum extract (CSE) against certain gastrointestinal cancer cell lines with no significant harmful effects on the normal cells. Method: This in vitro study was conducted to investigate the mechanism of action of CSE in SW742 (colon cancer cell line) using western blotting, real-time polymerase chain reaction (PCR), and PI staining/flow cytometry analysis. Results: Western blot analysis showed that CSE upregulates P53 protein expression significantly in SW742 cancer cell line, while QRT-PCR results revealed that P53 mRNA did not increase accordingly. It is proposed that TP53 protein play its role as a tumor suppressor through the protein stability mechanism and not through the increase in the gene expression. Considering this fact, for TP53 stabilization, two of its target genes, p21 and mdm2, were regulated. We evaluated the mRNA expression of these two genes as well. The obtained data showed a significant increase in both genes by CSE (1000 μg/ml), suggesting that the induction of G2 cell cycle arrest, which we previously reported, may happen through P53 and P21over-expression. No significant decrease was observed in apoptosis when SW742 cells were co-treated with CSE and the inhibitor of P53 transcriptional activity, (PFT)-α, indicating that the induction of apoptosis with CSE did not occur through P53-dependent transcriptional activity. Conclusion: The obtained results herein revealed that the observed anticancer effect of CSE may occur through TP53 upregulation, yet with P53-independent transcriptional activity.
format	article
author	Mojtaba Rashidi Atefeh Seghatoleslam
author_facet	Mojtaba Rashidi Atefeh Seghatoleslam
author_sort	Mojtaba Rashidi
title	Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway
title_short	Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway
title_full	Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway
title_fullStr	Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway
title_full_unstemmed	Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway
title_sort	induction of apoptosis with cyrtopodion scabrum extract in colon cancer cells: a preliminary study on targeting p53 signaling pathway
publisher	Shiraz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/5e28d9407da249108c2f43225fcc7b38
work_keys_str_mv	AT mojtabarashidi inductionofapoptosiswithcyrtopodionscabrumextractincoloncancercellsapreliminarystudyontargetingp53signalingpathway AT atefehseghatoleslam inductionofapoptosiswithcyrtopodionscabrumextractincoloncancercellsapreliminarystudyontargetingp53signalingpathway
_version_	1718407401661530112

Induction of Apoptosis with Cyrtopodion Scabrum Extract in Colon Cancer Cells: A Preliminary Study on Targeting P53 Signaling Pathway

Ejemplares similares