Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators

Thao Nguyen,1 Brittany Payan,1 Amarayca Zambrano,1 Yong Du,1 Maria Bondesson,2 Chandra Mohan11Biomedical Engineering Department, University of Houston, Houston, TX 77204, USA; 2Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN 47405, USACorrespondence: Chandra MohanD...

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Autores principales:	Nguyen T, Payan B, Zambrano A, Du Y, Bondesson M, Mohan C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Green Tea innate immunity animal models IL-1 TNF Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5e2e892a776c4ff183c39d64941bd0ab
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spelling	oai:doaj.org-article:5e2e892a776c4ff183c39d64941bd0ab2021-12-02T05:38:18ZEpigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators1178-7031https://doaj.org/article/5e2e892a776c4ff183c39d64941bd0ab2019-08-01T00:00:00Zhttps://www.dovepress.com/epigallocatechin-3-gallate-suppresses-neutrophil-migration-speed-in-a--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Thao Nguyen,1 Brittany Payan,1 Amarayca Zambrano,1 Yong Du,1 Maria Bondesson,2 Chandra Mohan11Biomedical Engineering Department, University of Houston, Houston, TX 77204, USA; 2Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN 47405, USACorrespondence: Chandra MohanDepartment of Biomedical Engineering, University of Houston, 3517 Cullen Blvd, Room 2004, Houston, TX 77204, USATel +1 713 743 3709Email cmohan@central.uh.eduBackground: Polyphenol catechins from green tea, particularly (−)-epigallocatechin-3-gallate (EGCG), exhibits numerous beneficial health effects, although the mechanisms remain unclear. Methods: In this study, the mechanism of EGCG-mediated healing in an experimentally injured zebrafish model was examined at the cellular and molecular level using confocal microscopy and gene expression analysis. Results: The mechanisms of action of EGCG were shown to involve: (1) reducing neutrophil response (accumulation, travel speed, and distance) and (2) downregulating the expression of IL-1β, TNFα, and related signaling pathways. As determined by dynamic time-lapse tracking studies, the local accumulation of neutrophils with high migration speeds after wounding (n=33 cells, v=0.020 μm/s, d=37.8 μm), underwent significant reduction following treatment with EGCG doses of 300 μM (n=22 cells, v=0.013 μm/s, d=39.5 μm) and 600 μM (n=18 cells, v=0.008 μm/s, d=9.53 μm). Reverse transcription polymerase chain reaction studies revealed that several signature genes in the IL-1β, TNFα, and related signaling pathways were downregulated after EGCG treatment. Conclusion: The convenience, transparency, and simplicity of the zebrafish model facilitate tracking of fluorescent neutrophils in real time, in order to monitor inflammation, and assess the impact of therapeutic agents.Keywords: green tea, innate immunity, animal models, IL-1, TNFNguyen TPayan BZambrano ADu YBondesson MMohan CDove Medical PressarticleGreen Teainnate immunityanimal modelsIL-1TNFPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 12, Pp 231-239 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Green Tea innate immunity animal models IL-1 TNF Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Green Tea innate immunity animal models IL-1 TNF Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Nguyen T Payan B Zambrano A Du Y Bondesson M Mohan C Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
description	Thao Nguyen,1 Brittany Payan,1 Amarayca Zambrano,1 Yong Du,1 Maria Bondesson,2 Chandra Mohan11Biomedical Engineering Department, University of Houston, Houston, TX 77204, USA; 2Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN 47405, USACorrespondence: Chandra MohanDepartment of Biomedical Engineering, University of Houston, 3517 Cullen Blvd, Room 2004, Houston, TX 77204, USATel +1 713 743 3709Email cmohan@central.uh.eduBackground: Polyphenol catechins from green tea, particularly (−)-epigallocatechin-3-gallate (EGCG), exhibits numerous beneficial health effects, although the mechanisms remain unclear. Methods: In this study, the mechanism of EGCG-mediated healing in an experimentally injured zebrafish model was examined at the cellular and molecular level using confocal microscopy and gene expression analysis. Results: The mechanisms of action of EGCG were shown to involve: (1) reducing neutrophil response (accumulation, travel speed, and distance) and (2) downregulating the expression of IL-1β, TNFα, and related signaling pathways. As determined by dynamic time-lapse tracking studies, the local accumulation of neutrophils with high migration speeds after wounding (n=33 cells, v=0.020 μm/s, d=37.8 μm), underwent significant reduction following treatment with EGCG doses of 300 μM (n=22 cells, v=0.013 μm/s, d=39.5 μm) and 600 μM (n=18 cells, v=0.008 μm/s, d=9.53 μm). Reverse transcription polymerase chain reaction studies revealed that several signature genes in the IL-1β, TNFα, and related signaling pathways were downregulated after EGCG treatment. Conclusion: The convenience, transparency, and simplicity of the zebrafish model facilitate tracking of fluorescent neutrophils in real time, in order to monitor inflammation, and assess the impact of therapeutic agents.Keywords: green tea, innate immunity, animal models, IL-1, TNF
format	article
author	Nguyen T Payan B Zambrano A Du Y Bondesson M Mohan C
author_facet	Nguyen T Payan B Zambrano A Du Y Bondesson M Mohan C
author_sort	Nguyen T
title	Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
title_short	Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
title_full	Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
title_fullStr	Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
title_full_unstemmed	Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
title_sort	epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/5e2e892a776c4ff183c39d64941bd0ab
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Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators

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