Impaired Dendritic Cell Homing in COVID-19
The high mortality of COVID-19 is mostly attributed to acute respiratory distress syndrome (ARDS), whose histopathological correlate is diffuse alveolar damage (DAD). Furthermore, severe COVID-19 is often accompanied by a cytokine storm and a disrupted response of the adaptive immune system. Studies...
Guardado en:
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/5e2ed39ce4a14ab0b3c8204ee0e61fc3 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:5e2ed39ce4a14ab0b3c8204ee0e61fc3 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:5e2ed39ce4a14ab0b3c8204ee0e61fc32021-11-04T05:26:02ZImpaired Dendritic Cell Homing in COVID-192296-858X10.3389/fmed.2021.761372https://doaj.org/article/5e2ed39ce4a14ab0b3c8204ee0e61fc32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.761372/fullhttps://doaj.org/toc/2296-858XThe high mortality of COVID-19 is mostly attributed to acute respiratory distress syndrome (ARDS), whose histopathological correlate is diffuse alveolar damage (DAD). Furthermore, severe COVID-19 is often accompanied by a cytokine storm and a disrupted response of the adaptive immune system. Studies aiming to depict this dysregulation have mostly investigated the peripheral cell count as well as the functionality of immune cells. We investigated the impact of SARS-CoV-2 on antigen-presenting cells using multiplexed immunofluorescence. Similar to MERS-CoV and SARS-CoV, SARS-CoV-2 appears to be impairing the maturation of dendritic cells (DCs). DC maturation involves a switch in surface antigen expression, which enables the cells' homing to lymph nodes and the subsequent activation of T-cells. As quantitative descriptions of the local inflammatory infiltrate are still scarce, we compared the cell population of professional antigen-presenting cells (APC) in the lungs of COVID-19 autopsy cases in different stages of DAD. We found an increased count of myeloid dendritic cells (mDCs) in later stages. Interestingly, mDCs also showed no significant upregulation of maturation markers in DAD-specimens with high viral load. Accumulation of immature mDCs, which are unable to home to lymph nodes, ultimately results in an inadequate T-cell response.Lukas BorcherdingAlime Sema TeksenBianca GrosserTina SchallerKlaus HirschbühlRainer ClausOliver SpringMichael WittmannChristoph RömmeleÉva SiposBruno MärklFrontiers Media S.A.articledendritic cellsmaturationhomingSARS-CoV-2COVID-19diffuse alveolar damage (DAD)Medicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
dendritic cells maturation homing SARS-CoV-2 COVID-19 diffuse alveolar damage (DAD) Medicine (General) R5-920 |
spellingShingle |
dendritic cells maturation homing SARS-CoV-2 COVID-19 diffuse alveolar damage (DAD) Medicine (General) R5-920 Lukas Borcherding Alime Sema Teksen Bianca Grosser Tina Schaller Klaus Hirschbühl Rainer Claus Oliver Spring Michael Wittmann Christoph Römmele Éva Sipos Bruno Märkl Impaired Dendritic Cell Homing in COVID-19 |
description |
The high mortality of COVID-19 is mostly attributed to acute respiratory distress syndrome (ARDS), whose histopathological correlate is diffuse alveolar damage (DAD). Furthermore, severe COVID-19 is often accompanied by a cytokine storm and a disrupted response of the adaptive immune system. Studies aiming to depict this dysregulation have mostly investigated the peripheral cell count as well as the functionality of immune cells. We investigated the impact of SARS-CoV-2 on antigen-presenting cells using multiplexed immunofluorescence. Similar to MERS-CoV and SARS-CoV, SARS-CoV-2 appears to be impairing the maturation of dendritic cells (DCs). DC maturation involves a switch in surface antigen expression, which enables the cells' homing to lymph nodes and the subsequent activation of T-cells. As quantitative descriptions of the local inflammatory infiltrate are still scarce, we compared the cell population of professional antigen-presenting cells (APC) in the lungs of COVID-19 autopsy cases in different stages of DAD. We found an increased count of myeloid dendritic cells (mDCs) in later stages. Interestingly, mDCs also showed no significant upregulation of maturation markers in DAD-specimens with high viral load. Accumulation of immature mDCs, which are unable to home to lymph nodes, ultimately results in an inadequate T-cell response. |
format |
article |
author |
Lukas Borcherding Alime Sema Teksen Bianca Grosser Tina Schaller Klaus Hirschbühl Rainer Claus Oliver Spring Michael Wittmann Christoph Römmele Éva Sipos Bruno Märkl |
author_facet |
Lukas Borcherding Alime Sema Teksen Bianca Grosser Tina Schaller Klaus Hirschbühl Rainer Claus Oliver Spring Michael Wittmann Christoph Römmele Éva Sipos Bruno Märkl |
author_sort |
Lukas Borcherding |
title |
Impaired Dendritic Cell Homing in COVID-19 |
title_short |
Impaired Dendritic Cell Homing in COVID-19 |
title_full |
Impaired Dendritic Cell Homing in COVID-19 |
title_fullStr |
Impaired Dendritic Cell Homing in COVID-19 |
title_full_unstemmed |
Impaired Dendritic Cell Homing in COVID-19 |
title_sort |
impaired dendritic cell homing in covid-19 |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doaj.org/article/5e2ed39ce4a14ab0b3c8204ee0e61fc3 |
work_keys_str_mv |
AT lukasborcherding impaireddendriticcellhomingincovid19 AT alimesemateksen impaireddendriticcellhomingincovid19 AT biancagrosser impaireddendriticcellhomingincovid19 AT tinaschaller impaireddendriticcellhomingincovid19 AT klaushirschbuhl impaireddendriticcellhomingincovid19 AT rainerclaus impaireddendriticcellhomingincovid19 AT oliverspring impaireddendriticcellhomingincovid19 AT michaelwittmann impaireddendriticcellhomingincovid19 AT christophrommele impaireddendriticcellhomingincovid19 AT evasipos impaireddendriticcellhomingincovid19 AT brunomarkl impaireddendriticcellhomingincovid19 |
_version_ |
1718445214052384768 |