Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)

Morphological alterations and nuclear abnormalities in fish erythrocytes have been used in many studies as bioindicators of environmental mutagens including ionizing radiation. In this study, adult Japanese medaka (Oryzias latipes) were irradiated with gamma rays at a low dose rate (9.92 μGy/min) fo...

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Autores principales:	Alaa El-Din H. Sayed, Kento Nagata, Takuya Nakazawa, Hiroshi Mitani, Junya Kobayashi, Shoji Oda
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	gamma-rays low dose-rate irradiation erythrocyte apoptosis medaka Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/5e300d0d66244ef390c45d37fd43fde2
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spelling	oai:doaj.org-article:5e300d0d66244ef390c45d37fd43fde22021-12-02T00:36:41ZLow Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)2296-774510.3389/fmars.2021.773481https://doaj.org/article/5e300d0d66244ef390c45d37fd43fde22021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.773481/fullhttps://doaj.org/toc/2296-7745Morphological alterations and nuclear abnormalities in fish erythrocytes have been used in many studies as bioindicators of environmental mutagens including ionizing radiation. In this study, adult Japanese medaka (Oryzias latipes) were irradiated with gamma rays at a low dose rate (9.92 μGy/min) for 7 days, giving a total dose of 100 mGy; and morphological alterations, nuclear abnormalities, and apoptotic cell death induced in peripheral erythrocytes were investigated 8 h and 7 days after the end of the irradiation. A variety of abnormalities, such as tear-drop cell, crenated cell, acanthocyte, sickled cell, micronucleated cell, eccentric nucleus, notched nucleus, and schistocyte, were induced in the peripheral erythrocytes of the wild-type fish, and a less number of abnormalities and apoptotic cell death were induced in the p53-deficient fish. These results indicate that low dose-rate chronic irradiation of gamma rays can induce cytotoxic and genotoxic effects in the peripheral erythrocytes of medaka, and p53-deficient medaka are tolerant to the gamma-ray irradiation than the wild type on the surface.Alaa El-Din H. SayedAlaa El-Din H. SayedKento NagataKento NagataTakuya NakazawaHiroshi MitaniJunya KobayashiJunya KobayashiShoji OdaFrontiers Media S.A.articlegamma-rayslow dose-rate irradiationerythrocyteapoptosismedakaScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gamma-rays low dose-rate irradiation erythrocyte apoptosis medaka Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	gamma-rays low dose-rate irradiation erythrocyte apoptosis medaka Science Q General. Including nature conservation, geographical distribution QH1-199.5 Alaa El-Din H. Sayed Alaa El-Din H. Sayed Kento Nagata Kento Nagata Takuya Nakazawa Hiroshi Mitani Junya Kobayashi Junya Kobayashi Shoji Oda Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)
description	Morphological alterations and nuclear abnormalities in fish erythrocytes have been used in many studies as bioindicators of environmental mutagens including ionizing radiation. In this study, adult Japanese medaka (Oryzias latipes) were irradiated with gamma rays at a low dose rate (9.92 μGy/min) for 7 days, giving a total dose of 100 mGy; and morphological alterations, nuclear abnormalities, and apoptotic cell death induced in peripheral erythrocytes were investigated 8 h and 7 days after the end of the irradiation. A variety of abnormalities, such as tear-drop cell, crenated cell, acanthocyte, sickled cell, micronucleated cell, eccentric nucleus, notched nucleus, and schistocyte, were induced in the peripheral erythrocytes of the wild-type fish, and a less number of abnormalities and apoptotic cell death were induced in the p53-deficient fish. These results indicate that low dose-rate chronic irradiation of gamma rays can induce cytotoxic and genotoxic effects in the peripheral erythrocytes of medaka, and p53-deficient medaka are tolerant to the gamma-ray irradiation than the wild type on the surface.
format	article
author	Alaa El-Din H. Sayed Alaa El-Din H. Sayed Kento Nagata Kento Nagata Takuya Nakazawa Hiroshi Mitani Junya Kobayashi Junya Kobayashi Shoji Oda
author_facet	Alaa El-Din H. Sayed Alaa El-Din H. Sayed Kento Nagata Kento Nagata Takuya Nakazawa Hiroshi Mitani Junya Kobayashi Junya Kobayashi Shoji Oda
author_sort	Alaa El-Din H. Sayed
title	Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)
title_short	Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)
title_full	Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)
title_fullStr	Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)
title_full_unstemmed	Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)
title_sort	low dose-rate irradiation of gamma-rays-induced cytotoxic and genotoxic alterations in peripheral erythrocytes of p53-deficient medaka (oryzias latipes)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5e300d0d66244ef390c45d37fd43fde2
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Low Dose-Rate Irradiation of Gamma-Rays-Induced Cytotoxic and Genotoxic Alterations in Peripheral Erythrocytes of p53-Deficient Medaka (Oryzias latipes)

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