Recent electroporation-based systems for intracellular molecule delivery

Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine. Among various delivery methods, recent years have seen the emergence of electroporation-based techniques that provide...

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Autores principales:	Zaizai Dong, Lingqian Chang
Formato:	article
Lenguaje:	EN
Publicado:	AIP Publishing LLC 2021
Materias:	Technology T Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/5e314351efbe4832b4cfdcb6de50d52c
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spelling	oai:doaj.org-article:5e314351efbe4832b4cfdcb6de50d52c2021-12-01T18:51:51ZRecent electroporation-based systems for intracellular molecule delivery2589-554010.1063/10.0005649https://doaj.org/article/5e314351efbe4832b4cfdcb6de50d52c2021-12-01T00:00:00Zhttp://dx.doi.org/10.1063/10.0005649https://doaj.org/toc/2589-5540Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine. Among various delivery methods, recent years have seen the emergence of electroporation-based techniques that provide versatile platforms for molecule delivery, with high efficiency and controlled dosage. In this Review, we describe recent electroporation-based systems for intracellular molecule delivery. The principles of electroporation for cell membrane perforation and cargo delivery are briefly summarized. Focusing on various scenarios for the application of electroporation, we review electroporation devices that variously employ structures based on nanochannels, nanostraws, and flow-through microfluidic channels for in vitro intracellular molecule delivery. We also consider in vivo targeted therapies based on delivery of active molecules by electroporation according to the lesion locations. Finally, we discuss the current challenges facing electroporation-based techniques, as well as opportunities for their future development, which may lead to innovations in intracellular molecule delivery both for cellular analysis in the laboratory and treatment in the clinic.Zaizai DongLingqian ChangAIP Publishing LLCarticleTechnologyTEngineering (General). Civil engineering (General)TA1-2040ENNanotechnology and Precision Engineering, Vol 4, Iss 4, Pp 045001-045001-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Technology T Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	Technology T Engineering (General). Civil engineering (General) TA1-2040 Zaizai Dong Lingqian Chang Recent electroporation-based systems for intracellular molecule delivery
description	Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine. Among various delivery methods, recent years have seen the emergence of electroporation-based techniques that provide versatile platforms for molecule delivery, with high efficiency and controlled dosage. In this Review, we describe recent electroporation-based systems for intracellular molecule delivery. The principles of electroporation for cell membrane perforation and cargo delivery are briefly summarized. Focusing on various scenarios for the application of electroporation, we review electroporation devices that variously employ structures based on nanochannels, nanostraws, and flow-through microfluidic channels for in vitro intracellular molecule delivery. We also consider in vivo targeted therapies based on delivery of active molecules by electroporation according to the lesion locations. Finally, we discuss the current challenges facing electroporation-based techniques, as well as opportunities for their future development, which may lead to innovations in intracellular molecule delivery both for cellular analysis in the laboratory and treatment in the clinic.
format	article
author	Zaizai Dong Lingqian Chang
author_facet	Zaizai Dong Lingqian Chang
author_sort	Zaizai Dong
title	Recent electroporation-based systems for intracellular molecule delivery
title_short	Recent electroporation-based systems for intracellular molecule delivery
title_full	Recent electroporation-based systems for intracellular molecule delivery
title_fullStr	Recent electroporation-based systems for intracellular molecule delivery
title_full_unstemmed	Recent electroporation-based systems for intracellular molecule delivery
title_sort	recent electroporation-based systems for intracellular molecule delivery
publisher	AIP Publishing LLC
publishDate	2021
url	https://doaj.org/article/5e314351efbe4832b4cfdcb6de50d52c
work_keys_str_mv	AT zaizaidong recentelectroporationbasedsystemsforintracellularmoleculedelivery AT lingqianchang recentelectroporationbasedsystemsforintracellularmoleculedelivery
_version_	1718404693253685248

Recent electroporation-based systems for intracellular molecule delivery

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