Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016

Abstract In 2014 the United States experienced a nationwide outbreak of Enterovirus D68 (EV-D68) infection. There were no confirmed cases of EV-D68 in 2015 and CDC was only aware of limited sporadic EV-D68 detection in the US in 2016. In this report, we analyzed 749 nasopharyngeal (NP) specimens col...

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Autores principales:	Guiqing Wang, Jian Zhuge, Weihua Huang, Sheila M. Nolan, Victoria L. Gilrane, Changhong Yin, Nevenka Dimitrova, John T. Fallon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e3633f1fe324a15919be07b64ee002e
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spelling	oai:doaj.org-article:5e3633f1fe324a15919be07b64ee002e2021-12-02T11:40:50ZEnterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 201610.1038/s41598-017-01349-42045-2322https://doaj.org/article/5e3633f1fe324a15919be07b64ee002e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01349-4https://doaj.org/toc/2045-2322Abstract In 2014 the United States experienced a nationwide outbreak of Enterovirus D68 (EV-D68) infection. There were no confirmed cases of EV-D68 in 2015 and CDC was only aware of limited sporadic EV-D68 detection in the US in 2016. In this report, we analyzed 749 nasopharyngeal (NP) specimens collected in 2015 and 2016 from patients in the Lower Hudson Valley, New York using a previously validated EV-D68-specific rRT-PCR assay. EV-D68 was detected in none of 199 NP specimens collected in 2015, and in one of 108 (0.9%) samples from January to May and 159 of 442 (36.0%) samples from July to October 2016. Complete EV-D68 genome sequences from 22 patients in 2016 were obtained using a metagenomic next-generation sequencing assay. Comparative genome analysis confirmed that a new EV-D68 strain belonging to subclade B3, with 3.2–4.8% divergence in nucleotide from subclade B1 strains identified during the 2014 US outbreak, was circulating in the US in 2016 and caused an outbreak in the Lower Hudson Valley, New York with 160 laboratory-confirmed cases. Our data highlight the genetic variability and capacity in causing outbreak by diverse EV-D68 strains, and the necessity of awareness and more surveillance on their active circulation worldwide.Guiqing WangJian ZhugeWeihua HuangSheila M. NolanVictoria L. GilraneChanghong YinNevenka DimitrovaJohn T. FallonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guiqing Wang Jian Zhuge Weihua Huang Sheila M. Nolan Victoria L. Gilrane Changhong Yin Nevenka Dimitrova John T. Fallon Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016
description	Abstract In 2014 the United States experienced a nationwide outbreak of Enterovirus D68 (EV-D68) infection. There were no confirmed cases of EV-D68 in 2015 and CDC was only aware of limited sporadic EV-D68 detection in the US in 2016. In this report, we analyzed 749 nasopharyngeal (NP) specimens collected in 2015 and 2016 from patients in the Lower Hudson Valley, New York using a previously validated EV-D68-specific rRT-PCR assay. EV-D68 was detected in none of 199 NP specimens collected in 2015, and in one of 108 (0.9%) samples from January to May and 159 of 442 (36.0%) samples from July to October 2016. Complete EV-D68 genome sequences from 22 patients in 2016 were obtained using a metagenomic next-generation sequencing assay. Comparative genome analysis confirmed that a new EV-D68 strain belonging to subclade B3, with 3.2–4.8% divergence in nucleotide from subclade B1 strains identified during the 2014 US outbreak, was circulating in the US in 2016 and caused an outbreak in the Lower Hudson Valley, New York with 160 laboratory-confirmed cases. Our data highlight the genetic variability and capacity in causing outbreak by diverse EV-D68 strains, and the necessity of awareness and more surveillance on their active circulation worldwide.
format	article
author	Guiqing Wang Jian Zhuge Weihua Huang Sheila M. Nolan Victoria L. Gilrane Changhong Yin Nevenka Dimitrova John T. Fallon
author_facet	Guiqing Wang Jian Zhuge Weihua Huang Sheila M. Nolan Victoria L. Gilrane Changhong Yin Nevenka Dimitrova John T. Fallon
author_sort	Guiqing Wang
title	Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016
title_short	Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016
title_full	Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016
title_fullStr	Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016
title_full_unstemmed	Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016
title_sort	enterovirus d68 subclade b3 strain circulating and causing an outbreak in the united states in 2016
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5e3633f1fe324a15919be07b64ee002e
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Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016

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