In-cell identification and measurement of RNA-protein interactions

RNA-interacting proteome can be identified by RNA affinity purification followed by mass spectrometry. Here the authors developed a different RNA-centric technology that combines high-throughput immunoprecipitation of RNA binding proteins and luciferase-based detection of their interaction with the...

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Autores principales:	Antoine Graindorge, Inês Pinheiro, Anna Nawrocka, Allison C. Mallory, Peter Tsvetkov, Noa Gil, Carlo Carolis, Frank Buchholz, Igor Ulitsky, Edith Heard, Mikko Taipale, Alena Shkumatava
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5e396512bb33457992f7bc69d0a77e2f
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spelling	oai:doaj.org-article:5e396512bb33457992f7bc69d0a77e2f2021-12-02T16:58:03ZIn-cell identification and measurement of RNA-protein interactions10.1038/s41467-019-13235-w2041-1723https://doaj.org/article/5e396512bb33457992f7bc69d0a77e2f2019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13235-whttps://doaj.org/toc/2041-1723RNA-interacting proteome can be identified by RNA affinity purification followed by mass spectrometry. Here the authors developed a different RNA-centric technology that combines high-throughput immunoprecipitation of RNA binding proteins and luciferase-based detection of their interaction with the RNA.Antoine GraindorgeInês PinheiroAnna NawrockaAllison C. MalloryPeter TsvetkovNoa GilCarlo CarolisFrank BuchholzIgor UlitskyEdith HeardMikko TaipaleAlena ShkumatavaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Antoine Graindorge Inês Pinheiro Anna Nawrocka Allison C. Mallory Peter Tsvetkov Noa Gil Carlo Carolis Frank Buchholz Igor Ulitsky Edith Heard Mikko Taipale Alena Shkumatava In-cell identification and measurement of RNA-protein interactions
description	RNA-interacting proteome can be identified by RNA affinity purification followed by mass spectrometry. Here the authors developed a different RNA-centric technology that combines high-throughput immunoprecipitation of RNA binding proteins and luciferase-based detection of their interaction with the RNA.
format	article
author	Antoine Graindorge Inês Pinheiro Anna Nawrocka Allison C. Mallory Peter Tsvetkov Noa Gil Carlo Carolis Frank Buchholz Igor Ulitsky Edith Heard Mikko Taipale Alena Shkumatava
author_facet	Antoine Graindorge Inês Pinheiro Anna Nawrocka Allison C. Mallory Peter Tsvetkov Noa Gil Carlo Carolis Frank Buchholz Igor Ulitsky Edith Heard Mikko Taipale Alena Shkumatava
author_sort	Antoine Graindorge
title	In-cell identification and measurement of RNA-protein interactions
title_short	In-cell identification and measurement of RNA-protein interactions
title_full	In-cell identification and measurement of RNA-protein interactions
title_fullStr	In-cell identification and measurement of RNA-protein interactions
title_full_unstemmed	In-cell identification and measurement of RNA-protein interactions
title_sort	in-cell identification and measurement of rna-protein interactions
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5e396512bb33457992f7bc69d0a77e2f
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In-cell identification and measurement of RNA-protein interactions

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