Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.

Yersinia pseudotuberculosis forms biofilms on Caenorhabditis elegans which block nematode feeding. This genetically amenable host-pathogen model has important implications for biofilm development on living, motile surfaces. Here we show that Y. pseudotuberculosis biofilm development on C. elegans is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Steve Atkinson, Robert J Goldstone, George W P Joshua, Chien-Yi Chang, Hannah L Patrick, Miguel Cámara, Brendan W Wren, Paul Williams
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5e3c2b6865c148caa34f70265b35f50a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5e3c2b6865c148caa34f70265b35f50a
record_format	dspace
spelling	oai:doaj.org-article:5e3c2b6865c148caa34f70265b35f50a2021-11-18T06:03:41ZBiofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.1553-73661553-737410.1371/journal.ppat.1001250https://doaj.org/article/5e3c2b6865c148caa34f70265b35f50a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21253572/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Yersinia pseudotuberculosis forms biofilms on Caenorhabditis elegans which block nematode feeding. This genetically amenable host-pathogen model has important implications for biofilm development on living, motile surfaces. Here we show that Y. pseudotuberculosis biofilm development on C. elegans is governed by N-acylhomoserine lactone (AHL)-mediated quorum sensing (QS) since (i) AHLs are produced in nematode associated biofilms and (ii) Y. pseudotuberculosis strains expressing an AHL-degrading enzyme or in which the AHL synthase (ypsI and ytbI) or response regulator (ypsR and ytbR) genes have been mutated, are attenuated. Although biofilm formation is also attenuated in Y. pseudotuberculosis strains carrying mutations in the QS-controlled motility regulator genes, flhDC and fliA, and the flagellin export gene, flhA, flagella are not required since fliC mutants form normal biofilms. However, in contrast to the parent and fliC mutant, Yop virulon proteins are up-regulated in flhDC, fliA and flhA mutants in a temperature and calcium independent manner. Similar observations were found for the Y. pseudotuberculosis QS mutants, indicating that the Yop virulon is repressed by QS via the master motility regulator, flhDC. By curing the pYV virulence plasmid from the ypsI/ytbI mutant, by growing YpIII under conditions permissive for type III needle formation but not Yop secretion and by mutating the type III secretion apparatus gene, yscJ, we show that biofilm formation can be restored in flhDC and ypsI/ytbI mutants. These data demonstrate that type III secretion blocks biofilm formation and is reciprocally regulated with motility via QS.Steve AtkinsonRobert J GoldstoneGeorge W P JoshuaChien-Yi ChangHannah L PatrickMiguel CámaraBrendan W WrenPaul WilliamsPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 1, p e1001250 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Steve Atkinson Robert J Goldstone George W P Joshua Chien-Yi Chang Hannah L Patrick Miguel Cámara Brendan W Wren Paul Williams Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.
description	Yersinia pseudotuberculosis forms biofilms on Caenorhabditis elegans which block nematode feeding. This genetically amenable host-pathogen model has important implications for biofilm development on living, motile surfaces. Here we show that Y. pseudotuberculosis biofilm development on C. elegans is governed by N-acylhomoserine lactone (AHL)-mediated quorum sensing (QS) since (i) AHLs are produced in nematode associated biofilms and (ii) Y. pseudotuberculosis strains expressing an AHL-degrading enzyme or in which the AHL synthase (ypsI and ytbI) or response regulator (ypsR and ytbR) genes have been mutated, are attenuated. Although biofilm formation is also attenuated in Y. pseudotuberculosis strains carrying mutations in the QS-controlled motility regulator genes, flhDC and fliA, and the flagellin export gene, flhA, flagella are not required since fliC mutants form normal biofilms. However, in contrast to the parent and fliC mutant, Yop virulon proteins are up-regulated in flhDC, fliA and flhA mutants in a temperature and calcium independent manner. Similar observations were found for the Y. pseudotuberculosis QS mutants, indicating that the Yop virulon is repressed by QS via the master motility regulator, flhDC. By curing the pYV virulence plasmid from the ypsI/ytbI mutant, by growing YpIII under conditions permissive for type III needle formation but not Yop secretion and by mutating the type III secretion apparatus gene, yscJ, we show that biofilm formation can be restored in flhDC and ypsI/ytbI mutants. These data demonstrate that type III secretion blocks biofilm formation and is reciprocally regulated with motility via QS.
format	article
author	Steve Atkinson Robert J Goldstone George W P Joshua Chien-Yi Chang Hannah L Patrick Miguel Cámara Brendan W Wren Paul Williams
author_facet	Steve Atkinson Robert J Goldstone George W P Joshua Chien-Yi Chang Hannah L Patrick Miguel Cámara Brendan W Wren Paul Williams
author_sort	Steve Atkinson
title	Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.
title_short	Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.
title_full	Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.
title_fullStr	Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.
title_full_unstemmed	Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.
title_sort	biofilm development on caenorhabditis elegans by yersinia is facilitated by quorum sensing-dependent repression of type iii secretion.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/5e3c2b6865c148caa34f70265b35f50a
work_keys_str_mv	AT steveatkinson biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT robertjgoldstone biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT georgewpjoshua biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT chienyichang biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT hannahlpatrick biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT miguelcamara biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT brendanwwren biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion AT paulwilliams biofilmdevelopmentoncaenorhabditiselegansbyyersiniaisfacilitatedbyquorumsensingdependentrepressionoftypeiiisecretion
_version_	1718424668090662912

Biofilm development on Caenorhabditis elegans by Yersinia is facilitated by quorum sensing-dependent repression of type III secretion.

Ejemplares similares