Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis

Background: The detection of anti-acetylcholine receptor (AChR) and anti-muscle-specific tyrosine kinase (MuSK) antibodies is useful in myasthenia gravis (MG) diagnosis and management. BIOCHIP mosaic-based indirect immunofluorescence is a novel analytical method, which employs the simultaneous detec...

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Autores principales:	Caterina Maria Gambino, Luisa Agnello, Bruna Lo Sasso, Concetta Scazzone, Rosaria Vincenza Giglio, Giuseppina Candore, Anna Maria Ciaccio, Vincenzo Di Stefano, Filippo Brighina, Matteo Vidali, Marcello Ciaccio
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	myasthenia gravis diagnosis biomarker anti-acetylcholine receptor antibodies anti-muscle-specific tyrosine kinase antibodies BIOCHIP Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5e48cbf01d4d4e1c8afe2d7bf23e72a4
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spelling	oai:doaj.org-article:5e48cbf01d4d4e1c8afe2d7bf23e72a42021-11-25T17:21:36ZComparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis10.3390/diagnostics111120982075-4418https://doaj.org/article/5e48cbf01d4d4e1c8afe2d7bf23e72a42021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2098https://doaj.org/toc/2075-4418Background: The detection of anti-acetylcholine receptor (AChR) and anti-muscle-specific tyrosine kinase (MuSK) antibodies is useful in myasthenia gravis (MG) diagnosis and management. BIOCHIP mosaic-based indirect immunofluorescence is a novel analytical method, which employs the simultaneous detection of anti-AChR and anti-MuSK antibodies in a single miniature incubation field. In this study, we compare, for the first time, the BIOCHIP MG mosaic with conventional enzyme-linked immunosorbent assay (ELISA) in the diagnosis of MG. Methods: A total of 71 patients with MG diagnosis were included in the study. Anti-AChR and anti-MuSK antibodies were measured separately by two different ELISA and simultaneously by BIOCHIP. The results were then compared. Results: The overall concordance between ELISA and BIOCHIP for anti-AChR reactivity was 74%. Cohen’s kappa was 0.51 (95% CI 0.32–0.71), which corresponds to 90% of the maximum possible kappa (0.57), given the observed marginal frequencies. The overall concordance for anti-MuSK reactivity was 84%. Cohen’s kappa was 0.11 (95% CI 0.00–0.36), which corresponds to 41% of the maximum possible kappa (0.27). Conclusion: The overall concordance among assays is not optimal.Caterina Maria GambinoLuisa AgnelloBruna Lo SassoConcetta ScazzoneRosaria Vincenza GiglioGiuseppina CandoreAnna Maria CiaccioVincenzo Di StefanoFilippo BrighinaMatteo VidaliMarcello CiaccioMDPI AGarticlemyasthenia gravisdiagnosisbiomarkeranti-acetylcholine receptor antibodiesanti-muscle-specific tyrosine kinase antibodiesBIOCHIPMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2098, p 2098 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	myasthenia gravis diagnosis biomarker anti-acetylcholine receptor antibodies anti-muscle-specific tyrosine kinase antibodies BIOCHIP Medicine (General) R5-920
spellingShingle	myasthenia gravis diagnosis biomarker anti-acetylcholine receptor antibodies anti-muscle-specific tyrosine kinase antibodies BIOCHIP Medicine (General) R5-920 Caterina Maria Gambino Luisa Agnello Bruna Lo Sasso Concetta Scazzone Rosaria Vincenza Giglio Giuseppina Candore Anna Maria Ciaccio Vincenzo Di Stefano Filippo Brighina Matteo Vidali Marcello Ciaccio Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis
description	Background: The detection of anti-acetylcholine receptor (AChR) and anti-muscle-specific tyrosine kinase (MuSK) antibodies is useful in myasthenia gravis (MG) diagnosis and management. BIOCHIP mosaic-based indirect immunofluorescence is a novel analytical method, which employs the simultaneous detection of anti-AChR and anti-MuSK antibodies in a single miniature incubation field. In this study, we compare, for the first time, the BIOCHIP MG mosaic with conventional enzyme-linked immunosorbent assay (ELISA) in the diagnosis of MG. Methods: A total of 71 patients with MG diagnosis were included in the study. Anti-AChR and anti-MuSK antibodies were measured separately by two different ELISA and simultaneously by BIOCHIP. The results were then compared. Results: The overall concordance between ELISA and BIOCHIP for anti-AChR reactivity was 74%. Cohen’s kappa was 0.51 (95% CI 0.32–0.71), which corresponds to 90% of the maximum possible kappa (0.57), given the observed marginal frequencies. The overall concordance for anti-MuSK reactivity was 84%. Cohen’s kappa was 0.11 (95% CI 0.00–0.36), which corresponds to 41% of the maximum possible kappa (0.27). Conclusion: The overall concordance among assays is not optimal.
format	article
author	Caterina Maria Gambino Luisa Agnello Bruna Lo Sasso Concetta Scazzone Rosaria Vincenza Giglio Giuseppina Candore Anna Maria Ciaccio Vincenzo Di Stefano Filippo Brighina Matteo Vidali Marcello Ciaccio
author_facet	Caterina Maria Gambino Luisa Agnello Bruna Lo Sasso Concetta Scazzone Rosaria Vincenza Giglio Giuseppina Candore Anna Maria Ciaccio Vincenzo Di Stefano Filippo Brighina Matteo Vidali Marcello Ciaccio
author_sort	Caterina Maria Gambino
title	Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis
title_short	Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis
title_full	Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis
title_fullStr	Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis
title_full_unstemmed	Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis
title_sort	comparative analysis of biochip mosaic-based indirect immunofluorescence with enzyme-linked immunosorbent assay for diagnosing myasthenia gravis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5e48cbf01d4d4e1c8afe2d7bf23e72a4
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Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis

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