Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.

<h4>Background</h4>Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called 'high-risk clusters'. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted...

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Autores principales:	Pascal Cholley, Roughyatou Ka, Christophe Guyeux, Michelle Thouverez, Nathalie Guessennd, Beniam Ghebremedhin, Thierry Frank, Xavier Bertrand, Didier Hocquet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e4dc385839f4f54ae74cc2a00ad31b8
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spelling	oai:doaj.org-article:5e4dc385839f4f54ae74cc2a00ad31b82021-11-25T06:01:55ZPopulation structure of clinical Pseudomonas aeruginosa from West and Central African countries.1932-620310.1371/journal.pone.0107008https://doaj.org/article/5e4dc385839f4f54ae74cc2a00ad31b82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25187957/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called 'high-risk clusters'. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted in Africa, where a very few isolates have been analyzed. Here, we characterized a collection of clinPA isolates from four countries of West and Central Africa.<h4>Methodology</h4>184 non-redundant isolates of clinPA from hospitals of Senegal, Ivory Coast, Nigeria, and Central African Republic were genotyped by MLST. We assessed their resistance level to antibiotics by agar diffusion and identified the extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) by sequencing. The population structure of the species was determined by a nucleotide-based analysis of the entire PA MLST database and further localized on the phylogenetic tree (i) the sequence types (STs) of the present collection, (ii) the STs by continents, (iii) ESBL- and MBL-producing STs from the MLST database.<h4>Principal findings</h4>We found 80 distinct STs, of which 24 had no relationship with any known STs. 'High-risk' international clonal complexes (CC155, CC244, CC235) were frequently found in West and Central Africa. The five VIM-2-producing isolates belonged to CC233 and CC244. GES-1 and GES-9 enzymes were produced by one CC235 and one ST1469 isolate, respectively. We showed the spread of 'high-risk' international clonal complexes, often described as multidrug-resistant on other continents, with a fully susceptible phenotype. The MBL- and ESBL-producing STs were scattered throughout the phylogenetic tree and our data suggest a poor association between a continent and a specific phylogroup.<h4>Conclusions</h4>ESBL- and MBL-encoding genes are borne by both successful international clonal complexes and distinct local STs in clinPA of West and Central Africa. Furthermore, our data suggest that the spread of a ST could be either due to its antibiotic resistance or to features independent from the resistance to antibiotics.Pascal CholleyRoughyatou KaChristophe GuyeuxMichelle ThouverezNathalie GuessenndBeniam GhebremedhinThierry FrankXavier BertrandDidier HocquetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107008 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pascal Cholley Roughyatou Ka Christophe Guyeux Michelle Thouverez Nathalie Guessennd Beniam Ghebremedhin Thierry Frank Xavier Bertrand Didier Hocquet Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.
description	<h4>Background</h4>Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called 'high-risk clusters'. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted in Africa, where a very few isolates have been analyzed. Here, we characterized a collection of clinPA isolates from four countries of West and Central Africa.<h4>Methodology</h4>184 non-redundant isolates of clinPA from hospitals of Senegal, Ivory Coast, Nigeria, and Central African Republic were genotyped by MLST. We assessed their resistance level to antibiotics by agar diffusion and identified the extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) by sequencing. The population structure of the species was determined by a nucleotide-based analysis of the entire PA MLST database and further localized on the phylogenetic tree (i) the sequence types (STs) of the present collection, (ii) the STs by continents, (iii) ESBL- and MBL-producing STs from the MLST database.<h4>Principal findings</h4>We found 80 distinct STs, of which 24 had no relationship with any known STs. 'High-risk' international clonal complexes (CC155, CC244, CC235) were frequently found in West and Central Africa. The five VIM-2-producing isolates belonged to CC233 and CC244. GES-1 and GES-9 enzymes were produced by one CC235 and one ST1469 isolate, respectively. We showed the spread of 'high-risk' international clonal complexes, often described as multidrug-resistant on other continents, with a fully susceptible phenotype. The MBL- and ESBL-producing STs were scattered throughout the phylogenetic tree and our data suggest a poor association between a continent and a specific phylogroup.<h4>Conclusions</h4>ESBL- and MBL-encoding genes are borne by both successful international clonal complexes and distinct local STs in clinPA of West and Central Africa. Furthermore, our data suggest that the spread of a ST could be either due to its antibiotic resistance or to features independent from the resistance to antibiotics.
format	article
author	Pascal Cholley Roughyatou Ka Christophe Guyeux Michelle Thouverez Nathalie Guessennd Beniam Ghebremedhin Thierry Frank Xavier Bertrand Didier Hocquet
author_facet	Pascal Cholley Roughyatou Ka Christophe Guyeux Michelle Thouverez Nathalie Guessennd Beniam Ghebremedhin Thierry Frank Xavier Bertrand Didier Hocquet
author_sort	Pascal Cholley
title	Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.
title_short	Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.
title_full	Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.
title_fullStr	Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.
title_full_unstemmed	Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.
title_sort	population structure of clinical pseudomonas aeruginosa from west and central african countries.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5e4dc385839f4f54ae74cc2a00ad31b8
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_version_	1718414284152635392

Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.

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