Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis

Scabies, a human skin infestation caused by the ectoparasitic mite Sarcoptes scabiei var. hominis, affects more than 200 million people globally. The prevailing knowledge of the disease process and host immune response mechanisms is limited. A better understanding of the host-parasite relationship i...

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Autores principales:	Huma Shehwana, Sadaf Ijaz, Abeera Fatima, Shelley Walton, Zafar Iqbal Sheikh, Waseem Haider, Shumaila Naz
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	RNA-seq data analysis Sarcoptes scabiei. var. hominis inflammatory responses scabies differentially expressed genes JAK-STAT pathways Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/5e52181bad5b4b3b9c643ade45a325ec
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spelling	oai:doaj.org-article:5e52181bad5b4b3b9c643ade45a325ec2021-12-01T23:44:11ZTranscriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis1664-322410.3389/fimmu.2021.778840https://doaj.org/article/5e52181bad5b4b3b9c643ade45a325ec2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.778840/fullhttps://doaj.org/toc/1664-3224Scabies, a human skin infestation caused by the ectoparasitic mite Sarcoptes scabiei var. hominis, affects more than 200 million people globally. The prevailing knowledge of the disease process and host immune response mechanisms is limited. A better understanding of the host-parasite relationship is essential for the identification of novel vaccine and drug targets. Here we aimed to interrogate the transcriptomic profiles of mite-infested human skin biopsies with clinical manifestations of ordinary scabies subjects (“OS”; n = 05) and subjects naive to scabies (“control”; n = 03) using RNASeq data analysis. A combined clustering, network, and pathway mapping approach enabled us to identify key signaling events in the host immune and pro-inflammatory responses to S. scabiei infestation. The clustering patterns showed various differentially expressed genes including inflammatory responses and innate immunity genes (DEFB4A, IL-19, CXCL8, CSF3, SERPINB4, S100A7A, HRNR) and notably upregulation of the JAK-STAT pathway in scabies-infested samples. Mite-infested human skin biopsies (GSE178563) were compared with an ex-vivo porcine infested model (E-MTAB-6433) and human skin equivalents (GSE48459). Marked enrichment of immune response pathways (JAK-STAT signaling, IL-4 and IL-13 pathway, and Toll receptor cascade), chemokine ligands and receptors (CCL17, CCL18, CCL3L1, CCL3L3, CCR7), and cytokines (IL-13 and IL-20) were observed. Additionally, genes known for their role in psoriasis and atopic dermatitis were upregulated, e.g., IL-19. The detailed transcriptomic profile has provided an insight into molecular functions, biological processes, and immunological responses and increased our understanding about transcriptomic regulation of scabies in human.Huma ShehwanaSadaf IjazAbeera FatimaShelley WaltonZafar Iqbal SheikhWaseem HaiderShumaila NazFrontiers Media S.A.articleRNA-seq data analysisSarcoptes scabiei. var. hominisinflammatory responsesscabiesdifferentially expressed genesJAK-STAT pathwaysImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA-seq data analysis Sarcoptes scabiei. var. hominis inflammatory responses scabies differentially expressed genes JAK-STAT pathways Immunologic diseases. Allergy RC581-607
spellingShingle	RNA-seq data analysis Sarcoptes scabiei. var. hominis inflammatory responses scabies differentially expressed genes JAK-STAT pathways Immunologic diseases. Allergy RC581-607 Huma Shehwana Sadaf Ijaz Abeera Fatima Shelley Walton Zafar Iqbal Sheikh Waseem Haider Shumaila Naz Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
description	Scabies, a human skin infestation caused by the ectoparasitic mite Sarcoptes scabiei var. hominis, affects more than 200 million people globally. The prevailing knowledge of the disease process and host immune response mechanisms is limited. A better understanding of the host-parasite relationship is essential for the identification of novel vaccine and drug targets. Here we aimed to interrogate the transcriptomic profiles of mite-infested human skin biopsies with clinical manifestations of ordinary scabies subjects (“OS”; n = 05) and subjects naive to scabies (“control”; n = 03) using RNASeq data analysis. A combined clustering, network, and pathway mapping approach enabled us to identify key signaling events in the host immune and pro-inflammatory responses to S. scabiei infestation. The clustering patterns showed various differentially expressed genes including inflammatory responses and innate immunity genes (DEFB4A, IL-19, CXCL8, CSF3, SERPINB4, S100A7A, HRNR) and notably upregulation of the JAK-STAT pathway in scabies-infested samples. Mite-infested human skin biopsies (GSE178563) were compared with an ex-vivo porcine infested model (E-MTAB-6433) and human skin equivalents (GSE48459). Marked enrichment of immune response pathways (JAK-STAT signaling, IL-4 and IL-13 pathway, and Toll receptor cascade), chemokine ligands and receptors (CCL17, CCL18, CCL3L1, CCL3L3, CCR7), and cytokines (IL-13 and IL-20) were observed. Additionally, genes known for their role in psoriasis and atopic dermatitis were upregulated, e.g., IL-19. The detailed transcriptomic profile has provided an insight into molecular functions, biological processes, and immunological responses and increased our understanding about transcriptomic regulation of scabies in human.
format	article
author	Huma Shehwana Sadaf Ijaz Abeera Fatima Shelley Walton Zafar Iqbal Sheikh Waseem Haider Shumaila Naz
author_facet	Huma Shehwana Sadaf Ijaz Abeera Fatima Shelley Walton Zafar Iqbal Sheikh Waseem Haider Shumaila Naz
author_sort	Huma Shehwana
title	Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
title_short	Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
title_full	Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
title_fullStr	Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
title_full_unstemmed	Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
title_sort	transcriptome analysis of host inflammatory responses to the ectoparasitic mite sarcoptes scabiei var. hominis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5e52181bad5b4b3b9c643ade45a325ec
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Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis

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