Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications

Abstract The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as To...

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Autores principales:	Saket Gupta, Ashwini Maratha, Jakub Siednienko, Anandan Natarajan, Thusitha Gajanayake, Shu Hoashi, Sinéad Miggin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e5f494fbf684e968ca4a373c602bbd9
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spelling	oai:doaj.org-article:5e5f494fbf684e968ca4a373c602bbd92021-12-02T16:08:11ZAnalysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications10.1038/s41598-017-07230-82045-2322https://doaj.org/article/5e5f494fbf684e968ca4a373c602bbd92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07230-8https://doaj.org/toc/2045-2322Abstract The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as Toll-like receptors (TLRs) and inflammatory mediators such as IL-6, TNFα and IL-1β have been linked with T2DM. Herein, we sought to establish whether patients with T2DM and underlying complications exhibit perturbations in cytokine and TLR expression. Serum cytokine and mRNA levels of cytokines/TLRs in monocytes (M) and neutrophils (N) were measured in a cohort of 112 diabetic patients: good glycaemic control without complications (GC), good glycaemic control with complications (GCC), poor glycaemic control without complications (PC) and poor glycaemic control with complications (PCC) and compared them with 34 non-diabetic volunteers (NGT). Serum cytokine levels were normal in all study participants. In the GC group, cytokine and TLR gene expression were enhanced compared to NGT. In contrast, suppressed cytokine and TLR gene expression were evident in PC, GCC & PCC groups when compared to the GC. In conclusion, whereas serum pro-inflammatory cytokine levels are unaltered in T2DM patients, differences in inflammatory gene profiles exist among the T2DM patient groups.Saket GuptaAshwini MarathaJakub SiednienkoAnandan NatarajanThusitha GajanayakeShu HoashiSinéad MigginNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saket Gupta Ashwini Maratha Jakub Siednienko Anandan Natarajan Thusitha Gajanayake Shu Hoashi Sinéad Miggin Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
description	Abstract The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as Toll-like receptors (TLRs) and inflammatory mediators such as IL-6, TNFα and IL-1β have been linked with T2DM. Herein, we sought to establish whether patients with T2DM and underlying complications exhibit perturbations in cytokine and TLR expression. Serum cytokine and mRNA levels of cytokines/TLRs in monocytes (M) and neutrophils (N) were measured in a cohort of 112 diabetic patients: good glycaemic control without complications (GC), good glycaemic control with complications (GCC), poor glycaemic control without complications (PC) and poor glycaemic control with complications (PCC) and compared them with 34 non-diabetic volunteers (NGT). Serum cytokine levels were normal in all study participants. In the GC group, cytokine and TLR gene expression were enhanced compared to NGT. In contrast, suppressed cytokine and TLR gene expression were evident in PC, GCC & PCC groups when compared to the GC. In conclusion, whereas serum pro-inflammatory cytokine levels are unaltered in T2DM patients, differences in inflammatory gene profiles exist among the T2DM patient groups.
format	article
author	Saket Gupta Ashwini Maratha Jakub Siednienko Anandan Natarajan Thusitha Gajanayake Shu Hoashi Sinéad Miggin
author_facet	Saket Gupta Ashwini Maratha Jakub Siednienko Anandan Natarajan Thusitha Gajanayake Shu Hoashi Sinéad Miggin
author_sort	Saket Gupta
title	Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
title_short	Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
title_full	Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
title_fullStr	Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
title_full_unstemmed	Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
title_sort	analysis of inflammatory cytokine and tlr expression levels in type 2 diabetes with complications
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5e5f494fbf684e968ca4a373c602bbd9
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Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications

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