Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
Regenerative medicine: lacrimal gland epithelium in vitro One possible approach to treat dry eye diseases is to transplant lacrimal glands generated in vitro from human embryonic stem cells into patients. As a first step, we developed a novel method to generate lacrimal gland epithelium-like cells....
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| Autores principales: | , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/5e63bc8203864273a52118fb05352626 |
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| Sumario: | Regenerative medicine: lacrimal gland epithelium in vitro One possible approach to treat dry eye diseases is to transplant lacrimal glands generated in vitro from human embryonic stem cells into patients. As a first step, we developed a novel method to generate lacrimal gland epithelium-like cells. As a model, we first studied the gene expression patterns of mouse embryonic lacrimal gland and identified key transcription factors involved in the process. Subsequently, we introduced four transcription factors in the form of synthetic mRNAs into human embryonic stem cells and successfully generated lacrimal gland epithelium-like cells, which showed elongated cell shape and increased expression of markers for epithelia, branching morphogenesis, and lacrimal glands. This study suggests the possibility of treating dry eye diseases through regeneration of lacrimal gland from human pluripotent stem cells. |
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