Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells

Regenerative medicine: lacrimal gland epithelium in vitro One possible approach to treat dry eye diseases is to transplant lacrimal glands generated in vitro from human embryonic stem cells into patients. As a first step, we developed a novel method to generate lacrimal gland epithelium-like cells....

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Autores principales:	Masatoshi Hirayama, Shigeru B.H. Ko, Tetsuya Kawakita, Tomohiko Akiyama, Sravan K. Goparaju, Atsumi Soma, Yuhki Nakatake, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeto Shimmura, Kazuo Tsubota, Minoru S.H. Ko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/5e63bc8203864273a52118fb05352626
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spelling	oai:doaj.org-article:5e63bc8203864273a52118fb053526262021-12-02T14:22:13ZIdentification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells10.1038/s41514-016-0001-82056-3973https://doaj.org/article/5e63bc8203864273a52118fb053526262017-01-01T00:00:00Zhttps://doi.org/10.1038/s41514-016-0001-8https://doaj.org/toc/2056-3973Regenerative medicine: lacrimal gland epithelium in vitro One possible approach to treat dry eye diseases is to transplant lacrimal glands generated in vitro from human embryonic stem cells into patients. As a first step, we developed a novel method to generate lacrimal gland epithelium-like cells. As a model, we first studied the gene expression patterns of mouse embryonic lacrimal gland and identified key transcription factors involved in the process. Subsequently, we introduced four transcription factors in the form of synthetic mRNAs into human embryonic stem cells and successfully generated lacrimal gland epithelium-like cells, which showed elongated cell shape and increased expression of markers for epithelia, branching morphogenesis, and lacrimal glands. This study suggests the possibility of treating dry eye diseases through regeneration of lacrimal gland from human pluripotent stem cells.Masatoshi HirayamaShigeru B.H. KoTetsuya KawakitaTomohiko AkiyamaSravan K. GoparajuAtsumi SomaYuhki NakatakeMiki SakotaNana Chikazawa-NohtomiShigeto ShimmuraKazuo TsubotaMinoru S.H. KoNature PortfolioarticleGeriatricsRC952-954.6ENnpj Aging and Mechanisms of Disease, Vol 3, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Geriatrics RC952-954.6
spellingShingle	Geriatrics RC952-954.6 Masatoshi Hirayama Shigeru B.H. Ko Tetsuya Kawakita Tomohiko Akiyama Sravan K. Goparaju Atsumi Soma Yuhki Nakatake Miki Sakota Nana Chikazawa-Nohtomi Shigeto Shimmura Kazuo Tsubota Minoru S.H. Ko Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
description	Regenerative medicine: lacrimal gland epithelium in vitro One possible approach to treat dry eye diseases is to transplant lacrimal glands generated in vitro from human embryonic stem cells into patients. As a first step, we developed a novel method to generate lacrimal gland epithelium-like cells. As a model, we first studied the gene expression patterns of mouse embryonic lacrimal gland and identified key transcription factors involved in the process. Subsequently, we introduced four transcription factors in the form of synthetic mRNAs into human embryonic stem cells and successfully generated lacrimal gland epithelium-like cells, which showed elongated cell shape and increased expression of markers for epithelia, branching morphogenesis, and lacrimal glands. This study suggests the possibility of treating dry eye diseases through regeneration of lacrimal gland from human pluripotent stem cells.
format	article
author	Masatoshi Hirayama Shigeru B.H. Ko Tetsuya Kawakita Tomohiko Akiyama Sravan K. Goparaju Atsumi Soma Yuhki Nakatake Miki Sakota Nana Chikazawa-Nohtomi Shigeto Shimmura Kazuo Tsubota Minoru S.H. Ko
author_facet	Masatoshi Hirayama Shigeru B.H. Ko Tetsuya Kawakita Tomohiko Akiyama Sravan K. Goparaju Atsumi Soma Yuhki Nakatake Miki Sakota Nana Chikazawa-Nohtomi Shigeto Shimmura Kazuo Tsubota Minoru S.H. Ko
author_sort	Masatoshi Hirayama
title	Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
title_short	Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
title_full	Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
title_fullStr	Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
title_full_unstemmed	Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
title_sort	identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5e63bc8203864273a52118fb05352626
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Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells

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