Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans

Summary: Cell-Specific Mitochondria Affinity Purification (CS-MAP) enables isolation and purification of intact mitochondria from individual cell types of Caenorhabditis elegans. The approach is based on the cell-specific expression of a recombinant hemagglutinin (HA)-tag fused to the TOMM-20 protei...

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Autores principales:	Arnaud Ahier, Tessa Onraet, Steven Zuryn
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell separation/fractionation Metabolism Model Organisms Molecular Biology Protein Biochemistry Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/5e69eb7feac844608ff36dfe0651566a
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spelling	oai:doaj.org-article:5e69eb7feac844608ff36dfe0651566a2021-11-18T04:51:39ZCell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans2666-166710.1016/j.xpro.2021.100952https://doaj.org/article/5e69eb7feac844608ff36dfe0651566a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006584https://doaj.org/toc/2666-1667Summary: Cell-Specific Mitochondria Affinity Purification (CS-MAP) enables isolation and purification of intact mitochondria from individual cell types of Caenorhabditis elegans. The approach is based on the cell-specific expression of a recombinant hemagglutinin (HA)-tag fused to the TOMM-20 protein that decorates the surface of mitochondria, thereby allowing their immunomagnetic purification. This protocol describes the CS-MAP procedure performed on large populations of animals. The purified mitochondria are suitable for subsequent nucleic acid, protein, and functional analyses.For complete details on the use and execution of this protocol, please refer to Ahier et al. (2018, 2021).Arnaud AhierTessa OnraetSteven ZurynElsevierarticleCell BiologyCell separation/fractionationMetabolismModel OrganismsMolecular BiologyProtein BiochemistryScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100952- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell separation/fractionation Metabolism Model Organisms Molecular Biology Protein Biochemistry Science (General) Q1-390
spellingShingle	Cell Biology Cell separation/fractionation Metabolism Model Organisms Molecular Biology Protein Biochemistry Science (General) Q1-390 Arnaud Ahier Tessa Onraet Steven Zuryn Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
description	Summary: Cell-Specific Mitochondria Affinity Purification (CS-MAP) enables isolation and purification of intact mitochondria from individual cell types of Caenorhabditis elegans. The approach is based on the cell-specific expression of a recombinant hemagglutinin (HA)-tag fused to the TOMM-20 protein that decorates the surface of mitochondria, thereby allowing their immunomagnetic purification. This protocol describes the CS-MAP procedure performed on large populations of animals. The purified mitochondria are suitable for subsequent nucleic acid, protein, and functional analyses.For complete details on the use and execution of this protocol, please refer to Ahier et al. (2018, 2021).
format	article
author	Arnaud Ahier Tessa Onraet Steven Zuryn
author_facet	Arnaud Ahier Tessa Onraet Steven Zuryn
author_sort	Arnaud Ahier
title	Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
title_short	Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
title_full	Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
title_fullStr	Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
title_full_unstemmed	Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans
title_sort	cell-specific mitochondria affinity purification (cs-map) from caenorhabditis elegans
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5e69eb7feac844608ff36dfe0651566a
work_keys_str_mv	AT arnaudahier cellspecificmitochondriaaffinitypurificationcsmapfromcaenorhabditiselegans AT tessaonraet cellspecificmitochondriaaffinitypurificationcsmapfromcaenorhabditiselegans AT stevenzuryn cellspecificmitochondriaaffinitypurificationcsmapfromcaenorhabditiselegans
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Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans

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