Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy

Two high resolution, 3D imaging techniques were applied to visualize and characterize sterilizing grade dual-layer filtration of liposomes, enabling membrane structure to be related with function and performance. Two polyethersulfone membranes with nominal retention ratings of 650 nm and 200 nm were...

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Autores principales:	Thomas F. Johnson, Kyle Jones, Francesco Iacoviello, Stephen Turner, Nigel B. Jackson, Kalliopi Zourna, John H. Welsh, Paul R. Shearing, Mike Hoare, Daniel G. Bracewell
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sterile filtration liposomes X-ray computed tomography confocal microscopy Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/5e6df16a9d974592985319a92403ef7c
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spelling	oai:doaj.org-article:5e6df16a9d974592985319a92403ef7c2021-11-25T18:20:18ZLiposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy10.3390/membranes111109052077-0375https://doaj.org/article/5e6df16a9d974592985319a92403ef7c2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/905https://doaj.org/toc/2077-0375Two high resolution, 3D imaging techniques were applied to visualize and characterize sterilizing grade dual-layer filtration of liposomes, enabling membrane structure to be related with function and performance. Two polyethersulfone membranes with nominal retention ratings of 650 nm and 200 nm were used to filter liposomes of an average diameter of 143 nm and a polydispersity index of 0.1. Operating conditions including differential pressure were evaluated. X-ray computed tomography at a pixel size of 63 nm was capable of resolving the internal geometry of each membrane. The respective asymmetry and symmetry of the upstream and downstream membranes could be measured, with pore network modeling used to identify pore sizes as a function of distance through the imaged volume. Reconstructed 3D digital datasets were the basis of tortuous flow simulation through each porous structure. Confocal microscopy visualized liposome retention within each membrane using fluorescent dyes, with bacterial challenges also performed. It was found that increasing pressure drop from 0.07 MPa to 0.21 MPa resulted in differing fluorescent retention profiles in the upstream membrane. These results highlighted the capability for complementary imaging approaches to deepen understanding of liposome sterilizing grade filtration.Thomas F. JohnsonKyle JonesFrancesco IacovielloStephen TurnerNigel B. JacksonKalliopi ZournaJohn H. WelshPaul R. ShearingMike HoareDaniel G. BracewellMDPI AGarticlesterile filtrationliposomesX-ray computed tomographyconfocal microscopyChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 905, p 905 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sterile filtration liposomes X-ray computed tomography confocal microscopy Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	sterile filtration liposomes X-ray computed tomography confocal microscopy Chemical technology TP1-1185 Chemical engineering TP155-156 Thomas F. Johnson Kyle Jones Francesco Iacoviello Stephen Turner Nigel B. Jackson Kalliopi Zourna John H. Welsh Paul R. Shearing Mike Hoare Daniel G. Bracewell Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
description	Two high resolution, 3D imaging techniques were applied to visualize and characterize sterilizing grade dual-layer filtration of liposomes, enabling membrane structure to be related with function and performance. Two polyethersulfone membranes with nominal retention ratings of 650 nm and 200 nm were used to filter liposomes of an average diameter of 143 nm and a polydispersity index of 0.1. Operating conditions including differential pressure were evaluated. X-ray computed tomography at a pixel size of 63 nm was capable of resolving the internal geometry of each membrane. The respective asymmetry and symmetry of the upstream and downstream membranes could be measured, with pore network modeling used to identify pore sizes as a function of distance through the imaged volume. Reconstructed 3D digital datasets were the basis of tortuous flow simulation through each porous structure. Confocal microscopy visualized liposome retention within each membrane using fluorescent dyes, with bacterial challenges also performed. It was found that increasing pressure drop from 0.07 MPa to 0.21 MPa resulted in differing fluorescent retention profiles in the upstream membrane. These results highlighted the capability for complementary imaging approaches to deepen understanding of liposome sterilizing grade filtration.
format	article
author	Thomas F. Johnson Kyle Jones Francesco Iacoviello Stephen Turner Nigel B. Jackson Kalliopi Zourna John H. Welsh Paul R. Shearing Mike Hoare Daniel G. Bracewell
author_facet	Thomas F. Johnson Kyle Jones Francesco Iacoviello Stephen Turner Nigel B. Jackson Kalliopi Zourna John H. Welsh Paul R. Shearing Mike Hoare Daniel G. Bracewell
author_sort	Thomas F. Johnson
title	Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
title_short	Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
title_full	Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
title_fullStr	Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
title_full_unstemmed	Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
title_sort	liposome sterile filtration characterization via x-ray computed tomography and confocal microscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5e6df16a9d974592985319a92403ef7c
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_version_	1718411321826869248

Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy

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