AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics

Purpose: The risk signature composed of four lncRNA (AC093797.1, POLR2J4, AL121748.1, and AL162231.4.) can be used to predict the overall survival (OS) of patients with hepatocellular carcinoma (HCC). However, the clinical significance and biological function of AC093797.1 are still unexplored in HC...

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Autores principales:	Xiaoling Liu, Chenyu Wang, Qing Yang, Yue Yuan, Yunjian Sheng, Decheng Li, Suvash Chandra Ojha, Changfeng Sun, Cunliang Deng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	biomarker HCC AC093797.1 biological function long-non-coding RNA Genetics QH426-470
Acceso en línea:	https://doaj.org/article/5e74471c53f44b17b71954d4a1d70ca1
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spelling	oai:doaj.org-article:5e74471c53f44b17b71954d4a1d70ca12021-12-03T05:37:19ZAC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics1664-802110.3389/fgene.2021.778742https://doaj.org/article/5e74471c53f44b17b71954d4a1d70ca12021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.778742/fullhttps://doaj.org/toc/1664-8021Purpose: The risk signature composed of four lncRNA (AC093797.1, POLR2J4, AL121748.1, and AL162231.4.) can be used to predict the overall survival (OS) of patients with hepatocellular carcinoma (HCC). However, the clinical significance and biological function of AC093797.1 are still unexplored in HCC or other malignant tumors. In this study, we aimed to investigate the biological function of AC093797.1 in HCC and screen the candidate hub genes and pathways related to hepatocarcinogenesis.Methods: RT-qPCR was employed to detect AC093797.1 in HCC tissues and cell lines. The role of AC093797.1 in HCC was evaluated via the cell-counting kit-8, transwell, and wound healing assays. The effects of AC093797.1 on tumor growth in vivo were clarified by nude mice tumor formation experiments. Then, RNA-sequencing and bioinformatics analysis based on subcutaneous tumor tissue was performed to identify the hub genes and pathways associated with HCC.Results: The expression of AC093797.1 decreased in HCC tissues and cell lines, and patients with low expressed AC093797.1 had poor overall survival (OS). AC093797.1 overexpression impeded HCC cell proliferation, invasion, and migration in vitro and suppressed tumor growth in vivo. Compared with the control group, 710 differentially expressed genes (243 upregulated genes and 467 downregulated genes) were filtered via RNA-sequencing, which mainly enriched in amino acid metabolism, extracellular matrix structure constituents, cell adhesion molecules cams, signaling to Ras, and signaling to ERKs.Conclusion: AC093797.1 may inhibit cell proliferation, invasion, and migration in HCC by reprograming cell metabolism or regulating several pathways, suggesting that AC093797.1 might be a potential therapeutic and prognostic marker for HCC patients.Xiaoling LiuXiaoling LiuXiaoling LiuChenyu WangChenyu WangChenyu WangQing YangYue YuanYue YuanYue YuanYunjian ShengYunjian ShengYunjian ShengDecheng LiDecheng LiDecheng LiSuvash Chandra OjhaSuvash Chandra OjhaSuvash Chandra OjhaChangfeng SunChangfeng SunChangfeng SunCunliang DengCunliang DengCunliang DengFrontiers Media S.A.articlebiomarkerHCCAC093797.1biological functionlong-non-coding RNAGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomarker HCC AC093797.1 biological function long-non-coding RNA Genetics QH426-470
spellingShingle	biomarker HCC AC093797.1 biological function long-non-coding RNA Genetics QH426-470 Xiaoling Liu Xiaoling Liu Xiaoling Liu Chenyu Wang Chenyu Wang Chenyu Wang Qing Yang Yue Yuan Yue Yuan Yue Yuan Yunjian Sheng Yunjian Sheng Yunjian Sheng Decheng Li Decheng Li Decheng Li Suvash Chandra Ojha Suvash Chandra Ojha Suvash Chandra Ojha Changfeng Sun Changfeng Sun Changfeng Sun Cunliang Deng Cunliang Deng Cunliang Deng AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics
description	Purpose: The risk signature composed of four lncRNA (AC093797.1, POLR2J4, AL121748.1, and AL162231.4.) can be used to predict the overall survival (OS) of patients with hepatocellular carcinoma (HCC). However, the clinical significance and biological function of AC093797.1 are still unexplored in HCC or other malignant tumors. In this study, we aimed to investigate the biological function of AC093797.1 in HCC and screen the candidate hub genes and pathways related to hepatocarcinogenesis.Methods: RT-qPCR was employed to detect AC093797.1 in HCC tissues and cell lines. The role of AC093797.1 in HCC was evaluated via the cell-counting kit-8, transwell, and wound healing assays. The effects of AC093797.1 on tumor growth in vivo were clarified by nude mice tumor formation experiments. Then, RNA-sequencing and bioinformatics analysis based on subcutaneous tumor tissue was performed to identify the hub genes and pathways associated with HCC.Results: The expression of AC093797.1 decreased in HCC tissues and cell lines, and patients with low expressed AC093797.1 had poor overall survival (OS). AC093797.1 overexpression impeded HCC cell proliferation, invasion, and migration in vitro and suppressed tumor growth in vivo. Compared with the control group, 710 differentially expressed genes (243 upregulated genes and 467 downregulated genes) were filtered via RNA-sequencing, which mainly enriched in amino acid metabolism, extracellular matrix structure constituents, cell adhesion molecules cams, signaling to Ras, and signaling to ERKs.Conclusion: AC093797.1 may inhibit cell proliferation, invasion, and migration in HCC by reprograming cell metabolism or regulating several pathways, suggesting that AC093797.1 might be a potential therapeutic and prognostic marker for HCC patients.
format	article
author	Xiaoling Liu Xiaoling Liu Xiaoling Liu Chenyu Wang Chenyu Wang Chenyu Wang Qing Yang Yue Yuan Yue Yuan Yue Yuan Yunjian Sheng Yunjian Sheng Yunjian Sheng Decheng Li Decheng Li Decheng Li Suvash Chandra Ojha Suvash Chandra Ojha Suvash Chandra Ojha Changfeng Sun Changfeng Sun Changfeng Sun Cunliang Deng Cunliang Deng Cunliang Deng
author_facet	Xiaoling Liu Xiaoling Liu Xiaoling Liu Chenyu Wang Chenyu Wang Chenyu Wang Qing Yang Yue Yuan Yue Yuan Yue Yuan Yunjian Sheng Yunjian Sheng Yunjian Sheng Decheng Li Decheng Li Decheng Li Suvash Chandra Ojha Suvash Chandra Ojha Suvash Chandra Ojha Changfeng Sun Changfeng Sun Changfeng Sun Cunliang Deng Cunliang Deng Cunliang Deng
author_sort	Xiaoling Liu
title	AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics
title_short	AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics
title_full	AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics
title_fullStr	AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics
title_full_unstemmed	AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics
title_sort	ac093797.1 as a potential biomarker to indicate the prognosis of hepatocellular carcinoma and inhibits cell proliferation, invasion, and migration by reprogramming cell metabolism and extracellular matrix dynamics
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5e74471c53f44b17b71954d4a1d70ca1
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AC093797.1 as a Potential Biomarker to Indicate the Prognosis of Hepatocellular Carcinoma and Inhibits Cell Proliferation, Invasion, and Migration by Reprogramming Cell Metabolism and Extracellular Matrix Dynamics

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