Optofluidic device for the quantification of circulating tumor cells in breast cancer

Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics...

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Autores principales:	Eric Pedrol, Manuel Garcia-Algar, Jaume Massons, Moritz Nazarenus, Luca Guerrini, Javier Martínez, Airan Rodenas, Ana Fernandez-Carrascal, Magdalena Aguiló, Laura G. Estevez, Isabel Calvo, Ana Olano-Daza, Eduardo Garcia-Rico, Francesc Díaz, Ramon A. Alvarez-Puebla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e7ef0a2e645437eb3463b68c9d01e45
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spelling	oai:doaj.org-article:5e7ef0a2e645437eb3463b68c9d01e452021-12-02T15:06:27ZOptofluidic device for the quantification of circulating tumor cells in breast cancer10.1038/s41598-017-04033-92045-2322https://doaj.org/article/5e7ef0a2e645437eb3463b68c9d01e452017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04033-9https://doaj.org/toc/2045-2322Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics are integrated into the chip, for the fluorescence quantification of CTCs in real samples. To test the chip performance, two cell membrane targets, the epithelial cell adhesion molecule, EpCAM, and the receptor tyrosine-protein kinase, HER2, are selected. The efficiency of the platform is demonstrated on cell lines and in a variety of healthy donors and metastatic-breast cancer patients.Eric PedrolManuel Garcia-AlgarJaume MassonsMoritz NazarenusLuca GuerriniJavier MartínezAiran RodenasAna Fernandez-CarrascalMagdalena AguilóLaura G. EstevezIsabel CalvoAna Olano-DazaEduardo Garcia-RicoFrancesc DíazRamon A. Alvarez-PueblaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eric Pedrol Manuel Garcia-Algar Jaume Massons Moritz Nazarenus Luca Guerrini Javier Martínez Airan Rodenas Ana Fernandez-Carrascal Magdalena Aguiló Laura G. Estevez Isabel Calvo Ana Olano-Daza Eduardo Garcia-Rico Francesc Díaz Ramon A. Alvarez-Puebla Optofluidic device for the quantification of circulating tumor cells in breast cancer
description	Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics are integrated into the chip, for the fluorescence quantification of CTCs in real samples. To test the chip performance, two cell membrane targets, the epithelial cell adhesion molecule, EpCAM, and the receptor tyrosine-protein kinase, HER2, are selected. The efficiency of the platform is demonstrated on cell lines and in a variety of healthy donors and metastatic-breast cancer patients.
format	article
author	Eric Pedrol Manuel Garcia-Algar Jaume Massons Moritz Nazarenus Luca Guerrini Javier Martínez Airan Rodenas Ana Fernandez-Carrascal Magdalena Aguiló Laura G. Estevez Isabel Calvo Ana Olano-Daza Eduardo Garcia-Rico Francesc Díaz Ramon A. Alvarez-Puebla
author_facet	Eric Pedrol Manuel Garcia-Algar Jaume Massons Moritz Nazarenus Luca Guerrini Javier Martínez Airan Rodenas Ana Fernandez-Carrascal Magdalena Aguiló Laura G. Estevez Isabel Calvo Ana Olano-Daza Eduardo Garcia-Rico Francesc Díaz Ramon A. Alvarez-Puebla
author_sort	Eric Pedrol
title	Optofluidic device for the quantification of circulating tumor cells in breast cancer
title_short	Optofluidic device for the quantification of circulating tumor cells in breast cancer
title_full	Optofluidic device for the quantification of circulating tumor cells in breast cancer
title_fullStr	Optofluidic device for the quantification of circulating tumor cells in breast cancer
title_full_unstemmed	Optofluidic device for the quantification of circulating tumor cells in breast cancer
title_sort	optofluidic device for the quantification of circulating tumor cells in breast cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5e7ef0a2e645437eb3463b68c9d01e45
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Optofluidic device for the quantification of circulating tumor cells in breast cancer

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