Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires

Abstract Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosph...

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Autores principales:	Sanja Babić, Lara Čižmek, Aleksandra Maršavelski, Olga Malev, Maryline Pflieger, Ivančica Strunjak-Perović, Natalija Topić Popović, Rozelindra Čož-Rakovac, Polonca Trebše
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e7f36d784974374a79e7aed0446c08d
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spelling	oai:doaj.org-article:5e7f36d784974374a79e7aed0446c08d2021-12-02T10:48:03ZUtilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires10.1038/s41598-021-81789-12045-2322https://doaj.org/article/5e7f36d784974374a79e7aed0446c08d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81789-1https://doaj.org/toc/2045-2322Abstract Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4,6-dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4,6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments.Sanja BabićLara ČižmekAleksandra MaršavelskiOlga MalevMaryline PfliegerIvančica Strunjak-PerovićNatalija Topić PopovićRozelindra Čož-RakovacPolonca TrebšeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanja Babić Lara Čižmek Aleksandra Maršavelski Olga Malev Maryline Pflieger Ivančica Strunjak-Perović Natalija Topić Popović Rozelindra Čož-Rakovac Polonca Trebše Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
description	Abstract Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4,6-dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4,6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments.
format	article
author	Sanja Babić Lara Čižmek Aleksandra Maršavelski Olga Malev Maryline Pflieger Ivančica Strunjak-Perović Natalija Topić Popović Rozelindra Čož-Rakovac Polonca Trebše
author_facet	Sanja Babić Lara Čižmek Aleksandra Maršavelski Olga Malev Maryline Pflieger Ivančica Strunjak-Perović Natalija Topić Popović Rozelindra Čož-Rakovac Polonca Trebše
author_sort	Sanja Babić
title	Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
title_short	Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
title_full	Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
title_fullStr	Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
title_full_unstemmed	Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
title_sort	utilization of the zebrafish model to unravel the harmful effects of biomass burning during amazonian wildfires
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5e7f36d784974374a79e7aed0446c08d
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Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires

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